Polymorpshisms of XPC Gene and Risk of Primary Lung Cancer in Koreans / 결핵

BACKGROUND: DNA repair plays a crucial role in protection from cancer-causing agents. Therefore, a reduced DNA repair capacity can increase the susceptibility to lung cancer. The XPC gene contains 15 exons and encodes a 940 amino acid protein that plays a central role in DNA damage recognition of the nucleotide excision repair pathway, which is a major DNA repair mechanism removing the bulky-helix distorting DNA lesions caused by smoking. Recently several polymorphisms in the XPC gene were identified. In addition, it is possible that these polymorphisms may affect the DNA repair capacity, which modulate cancer susceptibility. The relationship between codon 499 and 939 polymorphisms, and a poly(AT) insertion/deletion polymorphism in the XPC gene, and the lung cancer risk were investigated. METHOD: The genotypes were determined using either PCR or PCR-RFLP analysis in 219 male lung cancer patients and 150 healthy males controls. RESULTS: The frequencies of the genotypes (Val499Ala, PAT and Lys939Gln) among the cases were not significantly different from those of the controls. There was no significant associantion between these polymorphism and the lung cancer risk when the analyses were stratified according to age, smoking status and the pack-years of smoking. Moreover, the genotypes had no apparent relationship with any of the histological types of lung cancer. There was a linkage disequilibrium among the Val499Ala, PAT and Lys939Gln polymorphisms. The PAT polymorphism had a strong linkage disequilibrium with the Lys939Gln polymorphism (kappa value=0.87). The XPC haplotypes showed no significant association with the lung cancer risk. CONCLUSION: These results suggest that XPC Val499Ala, PAT and Lys939Gln polymorphisms are not major contributors to the individual lung cancer susceptibility in Koreans.

Ser326Cys Polymorphism of hOGG1 Gene and Risk of Primary Lung Cancer in Koreans / 결핵

BACKGROUND: DNA repair plays a crucial role in protecting the genome from cancer-causing agents. Therefore, a reduced DNA repair capacity can increase the susceptibility to cancer. The human OGG1 (hOGG1) gene encod es DNA glycosylase/apurinic lyase and excise 8-hydroxyguanine, one of the major premutagenic DNA lesions, which is produced by oxygen radical forming agents including smoking. Recently several polymorphisms in the hOGG1 gene were identified, and it is possible that these polymorphisms may affect the DNA repair capacity and thus modulate cancer susceptibility. The relationship between the codon 326 polymorphism (Ser to Cys) in the hOGG1 gene and lung cancer risk was investigated. MATERIALS AND METHOD: The Ser326Cys genotypes were determined using PCR-RFLP analysis in 299 primary lung cancer patients and 186 healthy controls who were frequency (case:control=3:2) matched according to age and sex. RESULT: The frequencies of the Ser326Cys genotypes (Ser/Ser, Ser/Cys and Cys/Cys) among cases (23.4%, 51.8%, and 24.7%, respectively) were not significantly different from those among the controls (22.6%, 52.1% and 25.3%, respectively). When the analyses were stratified according to age, sex, smoking status and pack-years of smoking, no significant association between this polymorphism and lung cancer risk was found. Moreover, the Ser326Cys genotype showed no apparent relationship with any of the histological types of lung cancer. CONCLUSION: These result suggest that the hOGG1 Ser326Cys polymorphism is not a major contributor to individual lung cancer susceptibility in Koreans.

Simple Bone Cyst: Radiologic Changes after Injection of Steroid

PURPOSE: To evaluate radiographic changes and clinical effects after the injection of steroid injection into simple bone cyst. MATERIALS AND METHODS: We analyzed plain radiographic and CT findings after the injection of steroid (methylprednisolone acetate, MPA) into simple bone cyst. Twelve patients were involved; eight were males and four were females, and their ages ranged from 6 to 41 years. They were treated from one to seven times with an intracystic injection of MPA, 80-200mgs per injection. All patients were evaluated by plain film, and four by CT, and the mean follow-up period was 20 months. RESULTS: Postinjection plain radiographic findings were as follows: increased internal radiodensity (n=10), smaller cyst (n=8), cortical thickening (n=7), and radiodensity of double ring pattern (n=4). CT findings were as follows: increased internal attenuation (n=4), smaller cyst (n=4) and radiodensity of double ring pattern along the cyst wall (n=4). All patients improved clinically and radiologically, especially those with radiodensity of double ring pattern. CONCLUSION: The injection of steroid into simple bone cyst is an effective treatment. A new radiographic finding after injection is radiodensity of double ring pattern along the cyst wall, and we believe that this indicates progression of the healing process.

Ossified Soft Tissue Recurrence of Giant Cell Tumor: Three Case Report

We report three cases of recurrence of giant cell tumor characterized by ossification of surrounding soft tissue. On resection, the ossified rim in the soft tissue masses were pathognomonic of recurrence of giant cell tumor. Knowledge of this findings would help for accurate diagnosis and appropriate treatment.

A Relationship Between Amount of Joint Effusion, Disk Displacement and Presence of Pain in theTemporomandibular Joint: MR Imaging

PURPOSE: To correlate MR findings of amount of temporomandibular joint effusion with joint pain and disk displacement. MATERIALS AND METHODS: In 57 patients, 114 temporomandibular joints with symptoms of disorder(presence of clinical pain) were imaged. Closed and open mouth sagittal spin echo (SE) T1-weighted images (WI) and fast spin echo (FSE) T2-WI were obtained. We classified the amount of joint effusion into grades. Joint effusion was classified as either Grade I, II or III, as follours : in sagittal FSE T2-WI, a long diameter of joint effusion shorter than 1/3 of the diameter of the convex margin of temporal eminence was grade I; longer than 2/3 was grade III; between grade I and III was grade II. Disk displacement was classified as either with or without reduction in the open mouth position. These findings were correlated with one another and statistically analyzed. RESULTS: In 29 cases with joint pain (37%) and in 34 cases without pain (63%) there was no evidence of joint effusion; there were, however, more grade I joint effusion cases with pain (14 ; 60.9%) than without pain (9 : 39.1%). Cases of joint pain increased in proportion to grade of joint effusion, which was statistically significant (p<0.05). In joints without disk displacement, the largest grouping was that which showed no effusion (39 ; 72.2%) ; no joints showed grade III effusion. Cases of joint effusion decreased in proportion to grade of effusion. In joints with disk displacement, cases of joint effusion tended to increase in proportion to the grade of effusion. CONCLUSION: MR findings of amount of temporomandibular joint effusion correlate with joint pain and anterior disk displacement.