Factors Affecting the Expansion of Chondrocytes Harvested from Rabbit Auricle and Nasal Septum / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: In tissue engineering of the cartilage, cellular expansion is an essential step for creating useful amounts of tissue required. Many variables such as media composition, growth factors and cell seeding density have been utilized to stimulate the ability of cellular proliferation and to reduce the number of expansion in monolayer. The object of this study was to evaluate the effects of human serum (HS) and rabbit serum (RS) on cellular proliferation of auricular and nasal septal chondrocytes of the rabbit. SUBJECTS AND METHOD: Auricular and nasal septal chondrocytes of the New Zealand White Rabbit were expanded in monolayer in medium supplemented with 2% RS, 5% RS, 10% RS, or 10% HS with or without adding of growth factors. The effect of the supplements on the cellular proliferation was compared. RESULTS: The proliferation yield of chondrocytes grown in RS was comparable to that with HS. Both serums results in increased proliferation; however, the amount of cellular proliferation was not correlated with the concentration of the serum. The proliferation rate of nasal septal chondrocytes was higher than that of auricular chondrocytes. CONCLUSION: Both HS and RS resulted in the increased proliferation of rabbit auricular and nasal septal chondrocytes. The results show that the determination of appropriate types and concentrations of serum is required for cell expansion in constructing tissue-engineered cartilage.

A Case of Diffuse Large B-Cell Lymphoma of Sinonasal Tract with Acute Visual Disturbance / 대한이비인후과학회지

Malignant lymphoma of the sinonasal tract is relatively uncommon. In particular, B-cell lymphoma is hardly found in the Asian population although the NK/T-cell type of lymphoma is relatively commonly found. The authors experienced a case of diffuse large B-cell lymphoma originating from the ethmoid sinus, which caused the unexpected visual disturbance without nasal symptoms and/or systemic B symptoms, such as fever, night sweat, and weight loss. Diagnosis of B cell lymphoma is often delayed because it does not always show the specific symptoms of lymphoma. Therefore, starting with careful physical and imaging studies are always important while higly suspecting malignancy. Furthermore, early precise biopsy is of great important for precise diagnosis and proper management for the treatment of malignant lymphoma.

A Case of Auricular Chondroma

Extraskeletal chondroma is a rare benign tumor that develops in the soft tissues, and it manifests as a solitary subcutaneous nodule or a slowly growing tumor. It occurs preferentially in the hand and foot area of adults, and the development in the auricle is very rare. We report a rare case of chondroma that developed in the auricle, which were treated with wedge excision and reconstructed using a Burow's triangle, with a review of the literature.