[Effect of combination of achillea millefolium, artemisia absinthium and juglans regia leaves extracts on leishmania major [MRHO/IR/75/ER], in vitro]

Due to side effects of the drug and also tendency of people in using herbal medicine, this study aimed to evaluate the sinerjhist effects of three herbs Achillea millefolium, Artemisia absinthium and Juglans regia leaf combained extract on Leishmania major, so that in case of effectiveness in further studies possible alternative drugs can be formulated. Access to formulation drug without harmful chemical material and without side effects. In an experimental study four tubes were selected and the mixture of three herbs leaf extracts with concentrations of 25 mg/ml were prepared. After 6, 12, 24, 48 and 72 hours sample were tested for dead or alive and also for their mobility of the parasite. Data were analyzed by ANOVA test. This study showed that combaind extracts of these three herbs caused an increase in immobility of the parasites. The rate of immobility had direct relationship with time and immobility was continuously increased after 24, 48 and 72 hours. The result were suggestive that three herbs leaf hydroalcoholic extracts were significantly more effective in reduction of parasite, in vitro [p<0.05]; however, Glucantime after 24 hours and amphotericin B after 30 minute caused immobility of parasite versus 24 hour for plant extract. Our study showed that the extracts of three mentioned herbs had effect on growth of Leishmania major, however further studies are needed to show effects of these herbs in animal models and voluntary infected people

[Comparision effectivness of extracts of thyme, yarrow, henna and garlic on cutaneous leishmaniasis caused by l. major in animal model [BALB/C]]

Leishmaniasis is not as severe as other diseases, often lesions are selfhealing. For various reason as long lasting duration of lesions and treatment, disfiguring scar reminding, secondary infection in lesions, have comfortable treatment method and accessible with less cost, it is necessary various method of treatment with less side effect. The results of previous studies showed that Indoleanalogues, Terpens, Iridoids, Quinones and Alkaloid hemisynthetic derivatives have promising antileishmanial potential. These derivatives were found in Thyme, Henna Yarrow and Garlic. Access to formulation drug without harmful chemical material and without side effect. After Inoculation of parasite in base tail of mice, during of three weeks caused lesions in inoculation place, then animal divided to 9 groups. In this study we used 4 alchoholic extract solution 2 times a day. we checked lesions weekly for six week and meseaured size of lesion in the base of tail with kulis - vernieh. Data of study analyzed with Anova, Paired t test and schefeh. We observed significant difference between mean of lesion in before and after treatment in control, Yarrow [Bomadaran] and Thyme [Avishan] groups [p<0.05]. Paired t test showed that there were not significant difference between mean of lesion in after treatment between groups of treatment and glucantime groups [p>0.05]. Alchoholic extract to Avishan and Boomadaran have good effect on lesions treatment, we suggest that the study could be repeated with Thyme [Avishan] and Yarrow [Bomadaran] extract in gel or cream base in first stage of lesions appearance