Association of UGT1A1 gene G71R polymorphism with neonatal hyperbilirubinemia / 中国小儿急救医学

Objective:To explore the association between the G71R polymorphism of the UGT1A1 gene and neonatal hyperbilirubinemia. Methods:DNA was extracted from blood samples of 61 neonates with severe neonatal hyperbilirubinemia(severe neonatal hyperbilirubinemia group), 60 neonates with hyperbilirubinemia(hyperbilirubinemia group) and 62 healthy neonates(control group), the G71R mutation of UGT1A1 gene was analyzed by direct sequencing. Results:In severe neonatal hyperbilirubinemia group, there were 17 cases of homozygous mutation(A/A), 23 cases of heterozygous mutation(A/G) , and 21 cases of wild type(G/G) , with 28.87% homozygous mutation rate and 37.70% heterozygous mutation rate.In neonatal hyperbilirubinemia group, there were ten cases of homozygous mutation(A/A), 28 cases of heterozygous mutation(A/G) and 22 cases of wild type(G/G), with 16.67% homozygous mutation rate and 46.67% heterozygous mutation rate.In the control group, there were nine cases of homozygous mutation (A/A), 28 cases of heterozygous mutation(A/G) and 25 cases of wild type(G/G), among which the homozygous mutation rate was 14.52% and the heterozygous mutation rate was 45.16%.The genotype frequency( χ2=4.14, P=0.38)and allele frequency( χ2=2.47, P=0.29)of G71R in severe neonatal hyperbilirubinemia group, neonatal hyperbilirubinemia group and control group were not statistically significant. Conclusion:The G71R polymorphism of the UGT1A1 gene may not be significantly correlated with the prevalence of neonatal hyperbilirubinemia.

Biological macrofeatures and criterion thereof for fracture immobilization in Chinese Mongolian traditional osteopathy / 医用生物力学

Objective To study the biological macrofeatures and criterion thereof for fracture immobilization in Chinese Mongolian traditional osteopathy. Method The principles and methods of modern physiological psychology and biomechanics were used in this study to explore the biological macrofeatures and criterion thereof for fracture immobilization, based on the view of the harmony of human and nature (including a unity of body and function) in Chinese Mongolian traditional osteopathy. Results Chinese Mongolian traditional osteopathy implies the biological macrofeatures and criterion thereof for “dynamic immobilization” in fracture treatment, including the stability of structure and force catched, state of static and dynamic, forming and destroying of bone, physical and psychological stability. Therefore, it is a kind of non invasive and non shelter fixing method. Conclusions The biological macrofeature and criterion thereof for the fracture dynamic immobilization in fracture treatment, in Chinese Mongolian traditional osteopathy is not only the fundamental support for its inheritance up to now, but also could be a new attempt in modern fracture immobilization.