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Braz. j. infect. dis ; 25(2): 101543, 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1278568

RESUMO

ABSTRACT In the pandemic, rapid and accurate detection of SARS-CoV-2 is crucial in controlling the outbreak. Recent studies have shown a high detection rate using saliva/oral fluids as specimens for laboratory detection of the virus. We intended to evaluate the test performance of the Xpert Xpress SARS-CoV-2 cartridge assay in comparison to a conventional qRT-PCR testing, using saliva as biological specimen. Forty saliva samples from symptomatic participants were collected. Conventional qRT-PCR was performed for amplification of E and RdRp genes and the Xpert Xpress SARS-CoV-2 assay amplified E and N2 genes. In the conventional assay, the median cycle threshold value of the E gene was 34.9, and of the RdRp gene was 38.3. In the Xpert Xpress assay, the median cycle threshold value of the E gene was 29.7, and of the N2 gene was 31.6. These results can allow a broaden use of molecular tests for management of COVID-19 pandemic, especially in resources-limited settings.


Assuntos
Humanos , SARS-CoV-2 , COVID-19 , Saliva , Manejo de Espécimes , Nasofaringe , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Técnicas de Laboratório Clínico , Pandemias , Teste para COVID-19
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