Detection of circulating trichinella pseudospiralis antigen by enzyme linked immunosorbent assay [ELISA]

A modified double antibody sandwich ELISA was used as a sensitive technique for demonstrating the appearance of circulating antigen of T. pseudospiralis in the sera of infected mice. The sera were tested at 5 days and 2, 4, 6, 7, 8, 10 and 12 weeks PI. The antigen was first detected on the 5th day PI; its level increased to reach the 1st peak at 4 weeks, then declined at 6 weeks. A 2nd peak was observed at 8 weeks, followed by a sharp decline to give negative OD reading at 10 weeks and increased to a moderate level at 12 weeks PI