RESUMO
The efficacy of an actinomycete strain JB66 recovered from a sand dune soil from the Bikaner district of the Thar desert in inhibiting the growth of various bacterial pathogens was studied. The type strains Staphylococcus aureus, Shigella flexneri, Klebsiella pneumoniae and the clinical isolates Escherichia coli, multidrug-resistant S. aureus and P. vulgaris were included in the antimicrobial assays. Polyphasic characterization of JB66 isolate revealed its identity as Streptomyces (MH762010). It showed 88.99-89.24% sequence similarity with the other members of this genus and share the maximum (88.89%) similarity with Streptomyces sp. ATSC13. The strain JB66 was found to produce a high amount of extracellular L-asparaginase, catalase, gelatinase, protease, tyrosinase and urease enzymes. The partial chemical categorization of the methanolic crude extract of the JB66 strain led to the preliminary identification of various metabolic compounds. The thin-layer chromatography fractionation revealed the presence of prodigiosin pigment or chandramycin, cephalosporin or zeatin, daidzein, demethoxy rapamycin, 4,6-dihydroxy-7-methoxyisoflavone, munumbicins and amiclenomycin like compounds. Bio-autography revealed that the metabolites localized at the Rf values of 0.40, 0.46 and 0.53 in TLC profile had the actual bioactive fractions. UV-VIS spectrum absorbance maxima at 288 nm revealed the presence of an aromatic nucleus.
RESUMO
We report a case of live adult Bancroftian Filarial nematode removed from the sub conjunctival tissue of a 58-year-old woman from the temporal limbus of her left eye.
Assuntos
Animais , Túnica Conjuntiva/parasitologia , Doenças da Túnica Conjuntiva/parasitologia , Infecções Oculares Parasitárias/parasitologia , Feminino , Filariose/parasitologia , Humanos , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Oftalmológicos/métodos , Wuchereria bancrofti/isolamento & purificaçãoAssuntos
Obstrução das Vias Respiratórias/etiologia , Arachis/efeitos adversos , Brônquios , Criança , Pré-Escolar , Feminino , Alimentos/efeitos adversos , Corpos Estranhos/classificação , Humanos , Masculino , Atelectasia Pulmonar/etiologia , Enfisema Pulmonar/etiologia , Insuficiência Respiratória/etiologiaRESUMO
Renal involvement was studied in 70 patients with leprosy by urine analysis, detailed biochemical investigations and renal histopathology. Of these 70 patients, 40 had lepromatous and 30 had non-lepromatous leprosy. Creatinine clearance was reduced in 20 patients. Renal biopsies were studied in 50 cases (25 lepromatous, 25 non-lepromatous); of which in 13 cases (10 lepromatous, 3 non-lepromatous) abnormal histopathological lesions were found by light microscopy. Amyloidosis was seen in only one lepromatous patient. No acid-fast bacilli and leproma like lesion were demonstrated in any case.