Correlation study on HPLC fingerprint chromatograms of Astragali Radix decoction pieces-standard decoction-dispensing granules / 中草药

Objective: To establish an HPLC fingerprint spectrum of for decoction pieces-, standard decoction- and dispensing granules of Astragali Radix, and evaluate the correlation among them. Methods: HPLC method was used to determine the content of 7-hydroxy- 4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The common pattern of the three fingerprints was established and the correlation was evaluated through the control fingerprint spectrum. Results: The average contents of 7-hydroxy-4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in 20 batches of decoction pieces were 0.097, 0.482, 0.142, and 0.237 mg/g, respectively; The average content of these four index components in the standard decoction were 0.116, 0.912, 0.214, and 0.434 mg/g, respectively; The average content of these four index components in the dispensing granules were 0.088,, 0.623,, 0.160, and 0.289 mg/g, respectively. There were six common peaks in all fingerprint spectra of 20 batches of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The similarity between dispensing granules and standard decoction was 0.980, indicating that the components difference between them was very small. Conclusion: The study establishes an HPLC fingerprint spectrum, and itwhich reflects the whole picture of multi-component of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. It provides reference value for the identification and quality control of Astragalus dispensing granules of Astragalus Radix.

Research on compatibility rule of traditional Chinese medicine for treatment of hyperhomocysteinemia based on data mining / 中草药

Objective: To summarize the medication rules of traditional Chinese medicine for treatment of hyperhomocysteinemia (HHcy) by data mining. Methods: To collect the relevant literatures of TCM treatment of HHcy from CNKI, VIP, Wanfang Data, CBM from the establishment of the database to December 31, 2019. TCMSS V 2.5 was used to count the frequency of medication, common drug pairs and drug combinations; Association rules, improved mutual information method, complex system entropy cluster analysis were used to analyze drug association rules and core drug combinations; Unsupervised entropy hierarchical clustering was used to analyze the new prescriptions. Results: There were 156 prescriptions with 199 Chinese medicinal herbs and 46 Chinese medicinal herbs of them with functions of promoting blood circulation and removing blood stasis, promoting urination and draining dampness, pacifying liver and extingushing wind and tonic deficiency were used for more than 10 times,. A total of 33 high-frequency drug combinations were obtained through data mining, and the top five combinations were Poria-Gastrodiae Rhizoma, Chuanxiong Rhizoma-Angelicae Sinensis Radix, Chuanxiong Rhizoma-Astragali Radix, Carthami Flos-Chuanxiong Rhizoma and Chuanxiong Rhizoma-Gastrodiae Rhizoma. A total of 20 core drug combinations and 10 potential new prescriptions were obtained through evolution. Conclusion: In the treatment of HHcy in TCM, blood-activating and stasis-resolving medicines, urination-promoting and dampness-draining medicines, liver-pacifying and wind-extingushing medicines and tonic deficiency medicines were mostly used. These prescriptions show that the main methods of promoting blood circulation and removing blood stasis, and resolving phlegm and turbidity, as well as supplementing qi and invigorating spleen, promoting urination and draining dampness, removing phlegm and widening chest reflect the characteristics of addressing both the symptoms and root causes of TCM.

Classification and Identification of Cynomorii Herba from Different Producing Areas Based on Fourier Rransform Infrared Spectroscopy and Chemometrics / 中国实验方剂学杂志

Objective: To realize the classification and identification of Cynomorii Herba from different producing areas based on fourier transform infrared spectroscopy (FTIR) and chemometrics. Method: FTIR spectrum data of 106 batches of Cynomorii Herba from 12 cities in 5 provinces were collected by transmission method and preprocessed. The FTIR fingerprints of Cynomorii Herba were established, and spectrum analysis was performed. The FTIR similarities of Cynomorii Herba from different producing areas were calculated by correlation coefficient method. The first derivative (1D) spectrum of average FTIR of Cynomorii Herba from different producing areas were obtained. The soft independent modeling of class analog (SIMCA) model based on principal component analysis (PCA) was established by the preprocessed 1D spectrum data. The orthogonal partial least squares (OPLS) model was established by top 6 principal components. Result: The FTIR fingerprint trend and main absorption peaks of Cynomorii Herba from different producing areas were basically the same,and 16 common characteristic absorption peaks were recognized. Similarity and 1D spectrum of FTIR fingerprint of Cynomorii Herba from different producing areas showed significant and unique characteristics. The established SIMCA model can realize the classification and identification of Cynomorii Herba from different provinces,while OPLS model can realize accurate classification and identification of Cynomorii Herba in different cities. The classification and identification of Cynomorii Herba from 12 city producing areas showed obvious geographical clustering characteristics. Conclusion: The established method based on FTIR and chemometrics can realize the classification and identification of Cynomorii Herba from 12 cities.

Long-term intraocular pressure fluctuation of primary angle closure disease following laser peripheral iridotomy/iridoplasty / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Many studies indicated that short-term and long-term intraocular pressure (IOP) fluctuations in primary open angle glaucoma patients might lead to glaucomatous progression. However, seldom study has evaluated the long-term fluctuation of IOP in primary chronic angle closure diseases. The objective of this study was to investigate the long-term IOP fluctuation of primary angle closure diseases and its associations following laser peripheral iridotomy (LPI) with or without laser peripheral iridoplasty.</p><p><b>METHODS</b>A total of 158 patients with primary angle closure suspect (PACS, n = 21), primary angle closure (PAC, n = 81) and primary angle closure glaucoma (PACG, n = 55) had been treated by LPI with or without laser peripheral iridoplasty and followed up for more than 12 months. IOP was measured with Goldman applanation tonometer. Multivariate linear regression with generalized estimating equation (GEE) regression models was used to evaluate the association of long-term IOP fluctuation (maximum IOP minus minimum IOP) with gender, age, baseline IOP, baseline peripheral anterior synechia (PAS), baseline vertical cup/disc ratio (VCDR), baseline mean deviation (MD), need for IOP-lowering medications.</p><p><b>RESULTS</b>IOP fluctuation during follow-up in PACS, PAC and PACG groups were (4.83 ± 2.90), (5.67 ± 3.35), and (9.40 ± 7.14) mmHg, respectively. IOP fluctuation was strongly correlated with baseline IOP (r = 0.356, P < 0.001), PAS (r = 0.374, P < 0.001). IOP fluctuation was higher in patients with higher baseline IOP (0.18 mmHg per unit increase, 95%CI: 0.05 - 0.31 mmHg).</p><p><b>CONCLUSIONS</b>Long-term IOP fluctuation in PACG group was larger than that in PACS or PAC group. Eyes with higher baseline IOP were observed to have larger long-term IOP fluctuation.</p>