RESUMO
Objective To establish a new kinetic fluorimetry method for the determination of trace catechol in waste water. Methods Trace catechol inhibited the catalytic oxidation of pyronine Y by potassium periodate in the presence of vanadium(the catalyst) in H2SO4 medium. A linear relation appeared between the concentration of catachol and the changing of catalyzed reaction speed. A new inhibitory kinetic fluorimetry method for the determination of trace catechol in waste water was established. Results The linear range of determination was 10.0-1 500.0 ?g/L,and the detection limit was 7.8 ?g/L. The RSDs and recovery rates of the method were 2.42%-2.71%,and 98.3%-103.3% respectively. Conclusion This method is simple and sensitive for the determination of trace catechol in waste water with satisfactory results.
RESUMO
Objective To establish a new kinetic fluorimetry method for the determination of trace nickel in human hair samples. Methods The method was based on the catalysis of nickel on the oxidation of fluorescein(CF) by hydrogen peroxide in an almost neutral medium. As nickel is present, the fluorescence intensity of the solution decreases. Results The wavelength of excitation was 492 nm and the wavelength of emission was 516 nm. The reaction was optimized at 100 ℃ for 10 min. The linear range of determination was 4.0-80.0 ?g/L and the detection limit was 2.2 ?g/L . The RSDs and recovery rates of the method were 2.6%-3.2%, and 96.7%-102.8% respectively. Conclusion This method is simple, rapid and sensitive for the determination of trace nickel in human hair samples.