Expressions of NOS isoforms in the cavernous tissues of diabetic rat models / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the mechanism of diabetic erectile dysfunction (ED) and find new methods for its treatment by detecting the changes in nitric oxide synthase (NOS) isoforms and erectile function of diabetic rats and observing the effects of insulin and alpha-lipoic acid (LA) on it.</p><p><b>METHODS</b>Fifty male Sprague-Dawley rats were divided into Groups A (normal control, n=10), B (non-intervention diabetes mellitus, n=13), C (insulin intervention diabetes mellitus, n=12), and D (insulin + LA intervention, n=15). And the diabetic models were made by intraperitoneal injection of streptozocin (STZ). Eight weeks later, the erectile function of the rats was assessed following apomorphine injection and the contents of NOS isoforms in the erectile tissues measured by immunohistochemical staining.</p><p><b>RESULTS</b>All the rats of Group A showed a normal erectile function (100%). In comparison, those in Groups B, C and D exhibited a significantly decreased rate, 28.6% in Group B, 62.5% in Group C and 80.9% in Group D. The numbers of positive nNOS fibers and eNOS in the penile tissues per visual field were 86.7 and 9.6 in Group A, but only 36.5 and 3.3 in Group B, 52.7 and 5.7 in Group C, and 71.4 and 7.4 in Group D (P < 0.05). However, the expression of iNOS was significantly lower in Group A (6.9) than in Groups B (43.6), C (36.2) and D (19.3) (P < 0.05). Compared with Groups B and C, the erectile function and the expressions of nNOS and eNOS were markedly increased, while the expression of iNOS significantly decreased in Group D (P < 0.05).</p><p><b>CONCLUSION</b>Diabetes mellitus severely affects penile erectile function and the expressions of NOS isoforms in the cavernous tissues, for which hyperglycemia is mainly responsible. LA is proved obviously efficacious for diabetic ED, which might be related to its antioxidant effect.</p>

Expression of nerve growth factor in cavernous tissue and its effects on the treatment of rats with diabetic erectile dysfunction / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the content of nerve growth factor (NGF) in penis of rats with diabetic erectile dysfunction (DED) and apply rhNGF to treat the DED rat model to study the pathogenesis mechanism of DED and treatment effects by NGF for DED rat model.</p><p><b>METHODS</b>Fifty adult male SD rats were randomly selected to make up diabetic models. After 8 weeks, the mRNA and protein levels of NGF in rat penis were detected. Then the rest of rats were divided into 5 groups: normal control group, non-treated diabetes group, NGF treatment group, insulin treatment group, NGF plus insulin treatment group. After 8 weeks of treatment, intracavernous pressure (ICP) was measured. The content of neuronal nitric oxide synthase (nNOS) in the erectile tissue was also value- ated by immunohistochemistry staining.</p><p><b>RESULTS</b>Compared with those of normal control group, the mRNA content of NGF and protein in the penis of non-treated diabetic rats is increased significantly. Compared with non-treated diabetic group, ICP was much higher in the treated groups which received NGF or/and insulin therapy, and the changes in nNOS staining of those groups were the same.</p><p><b>CONCLUSION</b>The injury of pelvic splanchnic nerves in advanced stage of diabetes may lead to DED, which may be relevant to the abnormal of NGF level or the NGF receptors. The increased extent of NGF in penis of rat with DED suggests that erectile nerves be severely damaged by diabete and the increase of producing NGF couldn't compensate the needs for reproduction of the nerve fibers. The ability of combination of NGF with its receptor may also be damaged. It is helpful to use exogenous NGF to lessen the partly neuropathy and improve the erectile dysfunction of diabetic rats. The abnormal of NGF may play an important role in the pathogenesis of diabetic ED and its treatment.</p>

The results of surgery on HIV carriers with urinary system disease / 中华男科学杂志

<p><b>OBJECTIVE</b>To approach characteristics of performing operation on HIV carriers with urinary system diseases.</p><p><b>METHODS</b>To summarize author's experiences of surgery on 41 HIV carriers suffering urinary system diseases abroad from April 1996 to May 2004.</p><p><b>RESULTS</b>The 41 HIV carriers received HAART and were performed with corresponding operations, followed up from 4 to 30 months post-operatively. The 31 carriers have recovered well up to date, while 4 carriers died of AIDS. Among them, 2 patients with penis cancer who received a partial peotomy and a patient with renal tuberculosis receiving left nephrectomy were died of AIDS within 4-8 months after operations whose CD4+ T lymphocyte number was below 0.2 x 10(9)/L.</p><p><b>CONCLUSION</b>Prior to operation, HIV carriers should receive HAART ordinarily to control copy of the virus. The CD4+ T lymphocyte number is important for selecting a proper time for operation and deciding the further after surgery. We also take note to CD4+ T lymphocyte number to monitor progress of the AIDS. For those HIV carriers, endourologic surgery and laparoscopy should be taken so far as possible. Meanwhile, medical stuffs must pay more attention to preventing occupational infection during surgery.</p>