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Twenty one flavonoid glycosides were isolated and purified from n-butanol portion of the water extract of A. annua by various chromatographic techniques such as HP-20 macroporous adsorption resin, silica gel, ODS, Sephadex LH-20 gel column chromatography and preparative high performance liquid chromatography. Their structures were identified by analysis of physicochemical properties and spectral data, and determined as axillarin-7-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside (1), orientin (2), apigenin-6-C-β-D-glucopyranosyl-8-C-β-L-arabinopyranoside (3), apigenin-6-C-β-D-galactopyranosyl-8-C-β-L-arabinopyranoside (4), apigenin-6-C-β-L-arabinopyranosyl-8-C-β-D-glucopyranoside (5), apigenin-6-C-α-L-arabinofuranosyl-8-C-β-D-glucopyranoside (6), quercetin-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (7), apigenin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (8), vicenin-2 (9), patuletin-7-O-β-D-glucopyranoside (10), luteolin-6-C-glucopyranoside (11), vitexin (12), kaempferol-3-O-β-galactopyranosyl-(1→2)-β-glucopyranoside (13), quercetin-7-O-β-D-glucopyranoside (14), patuletin-3-O-β-D-glucopyranoside (15), 7-O-methyl-quercetagetin-6-O-β-D-glucopyranoside (16), quercetin-3-O-β-D-glucopyranoside (17), nepitrin (18), rutin (19), kaempferol-3-O-β-sophoroside (20), and patuletin-3-O-rutinoside (21). Compound 1 is a new compound, compounds 2, 4, 6, 7, 10, 11, 13, 15, 16, 18, 20 and 21 are isolated from A. annua for the first time. In the anti-inflammatory assay, compound 1 inhibited the release of IL-6 from LPS-induced RAW264.7 cells to significantly degrees with the high (100 μmol·L-1), medium (50 μmol·L-1), low (25 μmol·L-1) concentration.
RESUMO
Objective To explore the effects of trendelenburg position on intraocular pressure during robotic-assisted radical cystectomy and to analyze its influencing factors.Methods Forty-one patients scheduled for roboticassisted radical cystectomy were included.Perioperative IOP measurements were performed as follows:10 min after anesthesia induction with supine position(T1),10 min after maintaining trendelenburg position(T2),1 h,2 h and 3 h after maintaining Trendelenburg position (T3,T4,T5),10 min after supine position (T6),2 h after supine position(T7).Gender,age,BMI,duration and angle of trendelenburg position,and blood loss were recorded.Results Mean IOP after trendelenburg position was 24.88±2.61 mmHg,which was higher than normal.Mean IOP in Trendelenburg positioning were significantly higher than those at T1,T6,T7,and IOP increased with time.The influencing factors of IOP included duration and degree of Trendelenburg position,and blood loss.Conclusion IOP increased in patients undergoing robotic-assisted radical cystectomy in Trendelenburg position.
RESUMO
Objective To explore the effects of trendelenburg position on intraocular pressure during robotic-assisted radical cystectomy and to analyze its influencing factors.Methods Forty-one patients scheduled for roboticassisted radical cystectomy were included.Perioperative IOP measurements were performed as follows:10 min after anesthesia induction with supine position(T1),10 min after maintaining trendelenburg position(T2),1 h,2 h and 3 h after maintaining Trendelenburg position (T3,T4,T5),10 min after supine position (T6),2 h after supine position(T7).Gender,age,BMI,duration and angle of trendelenburg position,and blood loss were recorded.Results Mean IOP after trendelenburg position was 24.88±2.61 mmHg,which was higher than normal.Mean IOP in Trendelenburg positioning were significantly higher than those at T1,T6,T7,and IOP increased with time.The influencing factors of IOP included duration and degree of Trendelenburg position,and blood loss.Conclusion IOP increased in patients undergoing robotic-assisted radical cystectomy in Trendelenburg position.