Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 22
Filtrar
1.
Philippine Journal of Nursing ; : 76-80, 2017.
Artigo | WPRIM | ID: wpr-960322

RESUMO

This paper centers on the staff nurses' level of competency and patients' level of satisfaction. Descriptive comparative design was utilized covering 32 staff nurses and 37 maternity patients from an accredited government maternity hospital. Purposive sampling was used to select the respondents. The questionnaires that were employed came from the Department of Health's Philippine Nurse Certification Program Self Assessment Tool for Level 3 Certification in Maternal and Child Nursing. The profile of staff nurses includes gender, length of experience, and educational attainment. On the other hand, gravidity, parity, and age are the patients' profile. Informed consent was given to all respondents. Statistical tools that were used includes: frequency and percentage, mean, standard deviation, independent sample t-Test, one way analysis of variance, and Pearson's correlation. The result concluded that there was no significant difference in the staff nurses' level of competency and patients' level of satisfaction when their profiles were considered. Moreover, it can be elucidated that there was no significant relationship between the level of competency and level of satisfaction. It can be interpreted that staff nurses are highly competent and patients are highly satisfied in terms of Client Care, Management and Leadership, and Research. However, there were statements with moderately competent results namely assess degree of laceration and actively participates in the collection of data research process to improve Maternal and Child Nursing practice in work setting with a mean of 2.50 and 2.28 respectively. The researcher suggests enhancement program in the form of the following trainings: internal external suturing in coordination with Association of Nursing Service Administrators of the Philippines and Research Process in coordination with Philippine Nursing Research Society.


Assuntos
Humanos , Masculino , Feminino , Adulto , Liderança , Autoavaliação (Psicologia) , Número de Gestações , Paridade , Satisfação Pessoal , Maternidades , Lacerações , Sociedades de Enfermagem , Pesquisa em Enfermagem
2.
World Journal of Emergency Medicine ; (4): 281-286, 2017.
Artigo em Inglês | WPRIM | ID: wpr-789816

RESUMO

@#BACKGROUND: The purpose of this study was to assess the short-term efficacy of a 4-week ultrasound curriculum taught by American first-year medical students to general practitioners working in public health care clinics, or puskesmas, in Bandung, Indonesia. METHODS: We performed a prospective, observational study of Indonesian health care practitioners from public clinics in Bandung, Indonesia. These practitioners were enrolled in a 4-week ultrasound training course taught by first-year American medical students. A total of six sessions were held comprising of 38 ultrasound milestones. A pre-course and post-course written exam and practical exam was taken by each participant. RESULTS: We enrolled 41 clinicians in the course. The average pre-course exam score was 35.2% with a 2.4% pass rate, whereas the average post-course exam score was 82.0% with a 92.7% pass rate. The average practical score at the completion of the course was 83.2% (SD=0.145) with 82.9% of the class passing (score above 75.0%). CONCLUSION: Our data suggests that first-year medical students can effectively teach ultrasound to physicians in Indonesia using a 4-week intensive ultrasound training course. Future studies are needed to determine the amount of training required for proficiency and to evaluate the physicians' perceptions of the student-instructors' depth of knowledge and skil in point of cаre ultrаsound.

3.
World Journal of Emergency Medicine ; (4): 177-183, 2017.
Artigo em Inglês | WPRIM | ID: wpr-789803

RESUMO

@#BACKGROUND:The purpose of this study was to use point-of-care ultrasound (POCUS) to investigate the relationship between tobacco smoke exposure and the characteristics of the common carotid artery (CCA). The effect of both primary and secondary smoking on CCA properties was evaluated. METHODS:We performed a prospective cross-sectional study across 20 primary care clinics in Bandung, West Java, Indonesia in July 2016. Point of care ultrasound was performed on a convenience sample of Indonesian patients presenting to clinic. The CCA wall stiffness and carotid intima-media thickness (CIMT) were measured during diastole and systole. These measurements were correlated with smoke exposure and cardiovascular disease. RESULTS: We enrolled 663 patients in the study, with 426 patients enrolled in the smoking category and 237 patients enrolled in the second-hand smoke category. There was an overall positive correlation with the measured lifestyle factors and the ultrasound-measured variables in the group of individuals who smoked. For all variables, age seemed to contribute the most out of all of the lifestyle factors for the positive changes in CIMT and CCA wall stiffness. CONCLUSION:Our data yielded correlations between CCA properties and cardiovascular risk, as well as between CIMT and arterial stiffness. We were also able to demonstrate an increase in thickness of the CIMT in patients who have been exposed by tobacco through the use of ultrasound. Further large scale studies comparing patients with multiple cardiac risk factors need to be performed to confirm the utility of ultrasound findings of cardiovascular disease and stroke.

4.
Philippine Journal of Nursing ; : 76-80, 2017.
Artigo em Inglês | WPRIM | ID: wpr-633601

RESUMO

This paper centers on the staff nurses' level of competency and patients' level of satisfaction. Descriptive comparative design was utilized covering 32 staff nurses and 37 maternity patients from an accredited government maternity hospital. Purposive sampling was used to select the respondents. The questionnaires that were employed came from the Department of Health's Philippine Nurse Certification Program Self Assessment Tool for Level 3 Certification in Maternal and Child Nursing. The profile of staff nurses includes gender, length of experience, and educational attainment. On the other hand, gravidity, parity, and age are the patients' profile. Informed consent was given to all respondents. Statistical tools that were used includes: frequency and percentage, mean, standard deviation, independent sample t-Test, one way analysis of variance, and Pearson's correlation. The result concluded that there was no significant difference in the staff nurses' level of competency and patients' level of satisfaction when their profiles were considered. Moreover, it can be elucidated that there was no significant relationship between the level of competency and level of satisfaction. It can be interpreted that staff nurses are highly competent and patients are highly satisfied in terms of Client Care, Management and Leadership, and Research. However, there were statements with moderately competent results namely assess degree of laceration and actively participates in the collection of data research process to improve Maternal and Child Nursing practice in work setting with a mean of 2.50 and 2.28 respectively. The researcher suggests enhancement program in the form of the following trainings: internal external suturing in coordination with Association of Nursing Service Administrators of the Philippines and Research Process in coordination with Philippine Nursing Research Society.


Assuntos
Humanos , Masculino , Feminino , Adulto , Liderança , Autoavaliação (Psicologia) , Número de Gestações , Paridade , Satisfação Pessoal , Maternidades , Lacerações , Sociedades de Enfermagem , Pesquisa em Enfermagem
5.
Eng. sanit. ambient ; 19(1): 33-42, Jan-Mar/2014. tab, graf
Artigo em Português | LILACS | ID: lil-703066

RESUMO

Os modelos de apoio à decisão são importantes ferramentas que podem ser utilizadas para analisar alternativas tecnológicas na gestão e tratamento de resíduos sólidos urbanos (RSU) frente a diversas situações envolvendo aspectos políticos, econômicos, ambientais e sociais. Este estudo teve como objetivo propor tecnologias e arranjos tecnológicos para o tratamento adequado de RSU para a Região Sul do Brasil, por meio do levantamento de informações e uso de dois modelos de apoio à decisão: AHP (Analytic Hierarchy Process) e Promethee II (Preference Ranking Organization Method for Enrichment Evaluations). As tecnologias analisadas foram: reciclagem, compostagem, tratamento mecânico-biológico, digestão anaeróbia, incineração com geração de energia elétrica em ciclo combinado (energia elétrica e térmica), aterro sanitário com e sem geração de energia. Essas tecnologias foram hierarquizadas nos modelos com base em quatro critérios: ambientais, sociais, econômicos e políticos. Como resultado, foram propostos quatro arranjos tecnológicos possíveis para a Região Sul e que podem ser utilizados como referência para estudos nas demais regiões geográficas do Brasil. .


The decision support models are important tools that can be used to analyze technological alternatives in the management and treatment of municipal solid waste (MSW) against several situations involving political, economic, environmental and social aspects. This study aimed to propose alternatives for appropriate treatment of MSW in the South Region of Brazil by surveying information and using two decision support models: AHP (Analytic Hierarchy Process) and Promethee II (Preference Ranking Organization Method for Enrichment Evaluations). The studied technologies were: recycling, composting, mechanical-biological treatment, anaerobic digestion, incineration with electricity generation and combined cycle (electricity and heat) and landfill with and without power generation. These technologies were hierarchically ranked in the models based on four criteria: environmental, social, economic and political. As a result, we proposed four possible technological arrangements for the South Region and these can be used as reference for studies in other geographic regions of Brazil. .

6.
Asian Pac J Allergy Immunol ; 2008 Jun-Sep; 26(2-3): 165-70
Artigo em Inglês | IMSEAR | ID: sea-36738

RESUMO

This study presents two patients who developed anaphylaxis after eating mite-contaminated food, and also contains a survey of dust-mites contamination in flour samples from Singapore households. The clinical records of each patient was studied. Patient A developed anaphylaxis twenty minutes following the ingestion of home-made fried fish coated with Japanese flour, while Patient B developed similar life-threatening symptoms one hour after the ingestion of home baked scones. Both patients were NSAID-intolerant and had a history of allergic rhinitis. Skin prick tests showed a strong positive result for dust-mites and for extracts prepared from the ingested flour. Flour samples were also examined microscopically which revealed large numbers of live Dermatophagoides farinae dust-mites. A survey of 57 flour samples showed that 4 samples (7%) were contaminated with dust mites. The findings in the present study confirm that mite-contamination of flour exists in Singaporean households, and it may trigger anaphylaxis in susceptible individuals.


Assuntos
Adolescente , Adulto , Anafilaxia/etiologia , Angioedema , Animais , Antígenos de Dermatophagoides/imunologia , Feminino , Farinha , Contaminação de Alimentos , Humanos , Hipersensibilidade/complicações , Pyroglyphidae/imunologia , Sons Respiratórios , Singapura , Testes Cutâneos , Urticária
7.
Artigo em Inglês | IMSEAR | ID: sea-37720

RESUMO

Breast cancer is the commonest cancer affecting females in Malaysia, contributing 31% of all newly diagnosed cases amongst Malaysian women. The present retrospective cohort study evaluated the relationship between cerbB- 2 onco-protein overexpression with various tumour characteristics and survival rate of breast cancer patients treated at the Universiti Kebangsaan Malaysia Medical Centre (UKMMC) between 1996-2000. CerbB- 2 oncoprotein overexpression was determined by immunohistochemistry (IHC) and tumors showing 2+ positivity were verified by Fluorescence In Situ Hybridization (FISH). One hundred and seventy two patients were eligible for the study with a short-term follow-up (median) of 5.1 years. C-erbB-2 oncoprotein overexpression correlated with lymph node positivity, oestrogen receptor (ER) and progesterone receptor (PR) negativity. Univariate analyses showed shorter disease free survival (DFS) and overall survival (OS) in patients with cerbB- 2 oncoprotein overexpression, Malay ethnicity, higher tumour grade, lymph node positivity, ER and PR negativity. In a subgroup of patients with c-erbB-2 oncoprotein overexpression, a shorter OS was observed in those with lymph node positivity, ER and PR negativity. In multivariate prognostic analysis, lymph node status, ER status and tumour grading were the strongest independent prognostic factors for both OS and DFS. However, c-erbB-2 status was not a significantly independent prognostic factor, even in subsets with lymph node positive or negative group. C-erbB-2 oncoprotein overexpression correlated well with lymph node status, ER and PR. Shorter OS and DFS were significantly observed in patients with c-erbB-2 oncoprotein overexpression. Lymph node status, ER status and tumour grading were the only three independent prognostic factors for OS and DFS in this study. Although c-erbB-2 expression is obviously important from a biological standpoint, multivariate analysis showed that it is not an independent prognostic indicator in breast carcinoma in the local population.

9.
Rev. méd. Chile ; 131(10): 1183-1197, oct. 2003.
Artigo em Espanhol | LILACS | ID: lil-355976

RESUMO

We report a 25 years old woman with a four years history of end stage renal disease on hemodialysis. During the last two years of follow up, multiple tender nodules appeared in the left clavicle and ribs. These were diagnosed as brown tumors, secondary to osteitis fibrosa cystica, in the context of a secondary hyperparathyroidism. A subtotal parathyroidectomy was performed and a homologous parathyroid implant was done in the right forearm. The pathological study of the surgical pieces confirmed the presence of the brown tumors and parathyroid hyperplasia. Nine months after surgery, parathormone levels and brown tumor calcifications decreased significantly and bone mineral density increased by 20 per cent.


Assuntos
Humanos , Feminino , Adulto , Neoplasias Primárias Múltiplas/complicações , Distúrbio Mineral e Ósseo na Doença Renal Crônica/complicações , Glândulas Paratireoides/patologia , Hiperparatireoidismo Secundário/complicações , Hiperparatireoidismo Secundário/diagnóstico , Hiperparatireoidismo Secundário/cirurgia , Hiperplasia/complicações , Hiperplasia/diagnóstico , Neoplasias Primárias Múltiplas/diagnóstico , Distúrbio Mineral e Ósseo na Doença Renal Crônica/diagnóstico , Osteíte Fibrosa Cística/complicações , Osteíte Fibrosa Cística/diagnóstico
10.
Asian Pac J Allergy Immunol ; 2001 Dec; 19(4): 225-9
Artigo em Inglês | IMSEAR | ID: sea-36951

RESUMO

This study was aimed to investigate the sensitization pattern to a range of common allergens in young Singaporean children. A cross-sectional study involving 75 children aged below 3 years was carried out. They presented between December 1995 and April 2000 with symptoms of asthma, rhinitis, eczema, or food allergy. Their levels of allergen-specific serum IgE to a panel of foods (egg white, milk, soy protein, shrimp, wheat and peanut), pet dander, dust mites and cockroaches were measured with Pharmacia CAP System radioallergosorbent test kits. Serum IgE levels greater than 0.35 kU/l represented a positive result. Four children could not be tested with the complete panel because of insufficient serum. The prevalence of sensitization was highest for cow's milk (45.9%) followed by egg white (38.7%), dust mites Dermatophagoides pteronyssinus (31.4%) and Blomia tropicalis (25.5%). Sensitization to ingested allergens was significantly more prevalent in children aged 1 year or younger than in the older children (70.4% of those below 1 year, and 50% of those aged 1-3 years; p < 0.02). Sensitization to inhaled allergens, such as dust mites, was more likely to manifest as respiratory symptoms (allergic rhinitis and asthma), while ingested allergens were associated with gastrointestinal symptoms and eczema (p < 0.001). It was concluded that infants and young children are at high risk of sensitization to common environmental substances. Allergen avoidance is therefore important even in the very young. The prevalence of sensitization to food allergens is higher compared to inhalant allergens in young children.


Assuntos
Alérgenos/análise , Animais , Pré-Escolar , Baratas/imunologia , Poeira/imunologia , Exposição Ambiental , Hipersensibilidade Alimentar/etiologia , Humanos , Hipersensibilidade Imediata/etiologia , Imunoglobulina E/sangue , Lactente , Recém-Nascido , Masculino , Leite/imunologia , Ácaros/imunologia , Teste de Radioalergoadsorção , Hipersensibilidade Respiratória/etiologia , Singapura
11.
Southeast Asian J Trop Med Public Health ; 2000 Jun; 31(2): 307-9
Artigo em Inglês | IMSEAR | ID: sea-30964

RESUMO

Between September 1998 and May 1999, 265 cases of encephalitis were reported from among those involved in pig rearing. A few cases were also reported among abattoir workers. This raised questions of the risk of transmission among those who handled raw pork. A serosurvey was conducted among pork sellers in Seremban town, which is about 20 km from one of the pig rearing areas which had reported cases of encephalitis. It was found that out of the 28 pork sellers tested, only one tested positive for Nipah virus antibodies and that this pork seller also worked in an abattoir in the same district, removing the urinary bladders from slaughtered pigs. Based on these findings, it was concluded that the risk of transmission of the virus from handling raw pork appeared to be low.


Assuntos
Animais , Estudos Transversais , Surtos de Doenças , Encefalite Viral/epidemiologia , Feminino , Humanos , Malásia/epidemiologia , Masculino , Carne , Pessoa de Meia-Idade , Doenças Profissionais/epidemiologia , Infecções por Paramyxoviridae/epidemiologia , Paramyxovirinae , Fatores de Risco , Suínos
12.
Asian Pac J Allergy Immunol ; 1998 Jun-Sep; 16(2-3): 57-68
Artigo em Inglês | IMSEAR | ID: sea-37017

RESUMO

This study examined the healthcare utilization patterns for asthma in Singapore, a tropical island city-state, between 1986 and 1993. Asthma was ranked fifth among principal conditions with the highest number of discharges in Singapore (2.4 discharges per 1,000 population per annum). Among the 0-14 year-olds, asthma was ranked second, only after accidents and injuries, as the condition with the highest number of discharges (5.2 per 1,000). It was estimated that there were approximately 20,000 accident and emergency (A & E) room visits for asthma per annum (7.0 visits per 1,000 population per annum). There were, however, no significant changes in the number of hospitalized or emergency room cases for asthma over the period of analysis. In the primary healthcare setting, asthma ranked 6th among the leading conditions seen by the general practitioners and government polyclinics (15 visits per 100 population per annum). Among under 15 year olds, asthma (37 visits per 100 age-adjusted population per annum) ranked as the second leading condition behind upper respiratory tract infections. This study has shown that asthma causes a significant amount of morbidity in the Singapore community.


Assuntos
Adolescente , Adulto , Idoso , Asma/epidemiologia , Criança , Pré-Escolar , Serviço Hospitalar de Emergência/estatística & dados numéricos , Feminino , Serviços de Saúde/estatística & dados numéricos , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Morbidade/tendências , Prevalência , Atenção Primária à Saúde/tendências , Fatores de Risco , Singapura/epidemiologia
13.
Asian Pac J Allergy Immunol ; 1999 Sep; 17(3): 203-6
Artigo em Inglês | IMSEAR | ID: sea-37236

RESUMO

The study of food allergy in Singaporean children is still in its infancy. Confusion and misunderstanding is common among the public. Even so, we have found certain unique features regarding food allergy among Singaporean children. "Bird's nest" has been shown to be the most common cause of anaphylaxis requiring medical attention. This allergen has not been described before. Peanuts and tree nuts are extremely uncommon causes of anaphylaxis, unlike the West. However, the pattern of sensitization to foods in children as shown by skin prick test is similar to other Western populations. The reasons for the difference between the profile foods responsible for anaphylactic reactions in our population and those of the western population, despite the similarity in sensitization profiles, are still unclear.


Assuntos
Criança , Pré-Escolar , Eczema/complicações , Hipersensibilidade Alimentar/epidemiologia , Humanos , Lactente , Recém-Nascido , Educação de Pacientes como Assunto , Prevalência , Singapura/epidemiologia
14.
Asian Pac J Allergy Immunol ; 1999 Sep; 17(3): 143-53
Artigo em Inglês | IMSEAR | ID: sea-37230

RESUMO

Asthma is a common cause of childhood morbidity. The objective of the present study was to evaluate the factors associated with increased asthma morbidity among asthmatic children in Singapore. A cohort of primary school children (n = 6,404, aged 6-13 years) were evaluated using the American Thoracic Society and the Division of Lung Diseases of the National Heart, Lung and Blood Institute, USA (ATS-DLD) respiratory questionnaire. A total of 2,222 of 6,404 children (34.8%) was found to have reported symptoms of wheezing. Of these, 899/2,222 (40.5%) reported symptoms of "increased asthma morbidity". This was associated with the younger age group, male sex and higher socio-economic status. In addition, concurrent or past allergies were strongly associated with increased asthma morbidity, while premature birth and a history of prior childhood respiratory illnesses and Infections were predictive of greater asthma morbidity. No association was found between increased morbidity and presence of domestic pets, parental smoking, childcare attendance, and the season of birth.


Assuntos
Adolescente , Asma/epidemiologia , Criança , Estudos de Coortes , Tosse/epidemiologia , Escolaridade , Feminino , Humanos , Masculino , Morbidade , Inquéritos e Questionários , Sons Respiratórios , Fatores de Risco , Singapura/epidemiologia , Classe Social
15.
Asian Pac J Allergy Immunol ; 1999 Sep; 17(3): 179-88
Artigo em Inglês | IMSEAR | ID: sea-36997

RESUMO

Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the predominant domestic mites species in Singapore and Taiwan. This study aims to characterize and compare the mite sensitization profiles in both countries. Skin prick tests were performed on 203 Singaporeans with Dp and Bt crude extracts. In vitro IgE and IgG4 reactivity to extracts and specific allergens (Der p 1, Der p 2 Der p 5 and Blo t 5) were determined by immunoassays. Approximately 91% of the tested Singaporeans were skin test positive for both Bt and Dp. Both populations share similar frequencies of in vitro IgE reactivity to all the allergens tested, but they differ in the pattern and magnitude of allergen sensitization. Although Der p 1, Der p 2 and Blo t5 are major sensitizing allergens in both countries, Blo t 5 is a more potent one in Singapore, probably reflecting the high level of exposure to Bt. The unique major Bt and Dp allergens should be included for precise diagnosis and effective immuno-therapeutic treatment of mite allergy in both countries.


Assuntos
Adolescente , Adulto , Alérgenos/imunologia , Animais , Antígenos de Dermatophagoides , Criança , Ensaio de Imunoadsorção Enzimática , Glicoproteínas/imunologia , Humanos , Hipersensibilidade/imunologia , Imunoensaio , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Ácaros/imunologia , Singapura , Testes Cutâneos , Taiwan
16.
Asian Pac J Allergy Immunol ; 1999 Sep; 17(3): 239-42
Artigo em Inglês | IMSEAR | ID: sea-36858

RESUMO

The genetics of asthma and atopy is complex, but can be approached by studies of both candidate genes and mapping of susceptibility loci. Genetic factors conferring susceptibility to disease may vary among ethnic groups. We present our experience with some candidate gene studies for asthma and atopy and susceptibility locus mapping for linkage to chromosome 5q.


Assuntos
Povo Asiático/genética , Asma/etnologia , Citocinas/genética , Predisposição Genética para Doença , Humanos , Hipersensibilidade Imediata/etnologia
17.
Asian Pac J Allergy Immunol ; 1999 Sep; 17(3): 189-94
Artigo em Inglês | IMSEAR | ID: sea-36441

RESUMO

Blomia tropicalis is an important triggering factor for allergic diseases such as asthma, rhinitis and atopic dermatitis in tropical and subtropical regions, which climate favours the growth of this species. Our previous mite fauna study revealed that Blomia tropicalis is the most dominant species present in Singapore house dust The main objective of this study is to establish a mass culture of Blomia tropicalis for further characterization of the antigenic and molecular properties of this mite. Approximately one gram of mites could be obtained for every 300-gram of culture medium by culturing under natural condition with a mean annual temperature of 30 degrees C and a mean relative humidity of 80%, and harvested by modified Tullgren funnel. Allergen characterization by IgE immunoblot analysis with crude mite extracts showed some IgE reactivity differences between Blomia tropicalis mite extract from Singapore and Colombia. The possible reasons for these findings are the quality and source of the mite protein extracts used, or selective differences in the population under evaluation. Further, the atopic sera tested showed differences in the pattern and Intensity of IgE immunoblot reactivity to crude extracts of Blomia tropicalis and Dermatophagoides pteronyssinus, the other highly prevalent mite in Singapore. These data support the existence of species-specific allergens. In conclusion, we have been successful in setting up B. tropicalis mass cultures and have prepared extracts of high allergenicity.


Assuntos
Alérgenos/imunologia , Animais , Poeira , Eletroforese em Gel de Poliacrilamida , Humanos , Immunoblotting , Imunoglobulina E/imunologia , Microscopia Eletrônica de Varredura , Ácaros/crescimento & desenvolvimento
18.
Asian Pac J Allergy Immunol ; 1995 Dec; 13(2): 101-5
Artigo em Inglês | IMSEAR | ID: sea-36828

RESUMO

Skin prick tests done previously revealed a significantly higher percentage of sensitization to an extract of Bipolaris sp. among atopic individuals (34/147, 23.1%) compared to non-atopic individuals. Bipolaris-specific IgE levels were quantified in sera from a representative group of 38 individuals using the Fluorescence Allergosorbent Test (FAST). Result obtained by FAST were found to be comparable to the skin prick test results (r2 = 0.60, p < 0.001 for IgE levels vs wheal sizes; r2 = 0.44, p < 0.001 for IgE levels vs erythema sizes). Characterisation of the extract's allergenic component by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed 28 protein bands with molecular weights (MW) ranging from 11 kDa to above 100 kDa. Immunoblotting with sera of 10 Bipolaris-sensitive (skin prick test, 3 +) individuals showed that Bipolaris spore extract contained at least 4 IgE binding proteins (MW 11-13 kDa, 16-17 kDa, 20-22 kDa and 36 kDa). All 10 sera reacted to the protein at MW 20-22 kDa, 2 sera with MW 11-13 kDa, 3 sera with 16-17 kDa and 6 sera with 36 kDa. This study has thus demonstrated that spores of Bipolaris sp. contain allergenic components which may elicit IgE-mediated reactions.


Assuntos
Alérgenos/química , Fungos/imunologia , Humanos , Hipersensibilidade Imediata/imunologia , Imunoglobulina E/sangue , Peso Molecular , Singapura , Testes Cutâneos
19.
Southeast Asian J Trop Med Public Health ; 1995 Jun; 26(2): 247-52
Artigo em Inglês | IMSEAR | ID: sea-34771

RESUMO

Two gene sequences specific for Mycobacterium tuberculosis were evaluated for the diagnosis of pulmonary tuberculous (PTB) in pleural fluid (PF), bronchoalveolar lavage fluid (BAL) and sputum (Sp). The 240 bp sequence (nts 460-700) coding for the MPB 64 protein coding gene and the 123 bp IS6110 insertion element present in multiple copies in the mycobacterial genome were amplified using the polymerase chain reaction. Fifty-nine clinical specimens were studied. The diagnosis of PTB was confirmed by positive M. tuberculosis cultures in 14 specimens, and by the presence of characteristic histological features of granuloma and Langerhan's giant cells on pleural biopsy in 3 PF specimens through cultures for M. tuberculosis were negative. The remaining 42 specimens were obtained from patient's with non-tuberculosis pulmonary infections or malignancy, and these served as negative controls. Our results showed that the IS6110 insertion element and MPB 64 gene sequence were detected in all 14 culture positive PTB cases, although detection of the latter sequence required both DNA amplification and oligonucleotide hybridization. There was however one false positive specimen with the MPB 64 detection protocol. More importantly, both the MPB 64 sequence and IS6110 insertion element protocols were unable to detect M. tuberculosis DNA in the 3 PF samples diagnosed by histological characteristics on pleural biopsy and culture negative. We conclude that DNA amplification for M. tuberculosis-specific sequences is a useful method for rapid diagnosis of PTB in culture positive specimens. However, the false negative results with TB culture negative cases of tuberculosis pleurisy, limits its usefulness for the diagnosis of tuberculous pleurisy.


Assuntos
Sequência de Bases , Líquido da Lavagem Broncoalveolar/microbiologia , Estudos de Casos e Controles , Elementos de DNA Transponíveis/genética , DNA Bacteriano/genética , Genes Bacterianos/genética , Humanos , Dados de Sequência Molecular , Mycobacterium tuberculosis/genética , Sondas de Oligonucleotídeos , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Singapura , Escarro/microbiologia , Tuberculose Pulmonar/diagnóstico
20.
Asian Pac J Allergy Immunol ; 1994 Dec; 12(2): 145-50
Artigo em Inglês | IMSEAR | ID: sea-37108

RESUMO

House dust mite allergens constitute one of the most important allergens in house dust. In this study, the levels of two common dust mite allergens, Der p I and Der f I, in a general hospital in Singapore were evaluated. Our results showed that these allergens were detected in 42/74 (or 57%) of the dust samples. Der p I was found to be the predominant allergen detected (p < 0.001). The allergen levels were, however, low with only 1/74 having a Der p I concentration above 2 micrograms g-1 dust. None of the samples had Der f I concentrations above this level. Of the various niches studied (mattresses, pillows, sofas, carpets, blinds and floors), the blinds and floors had the lowest concentration of allergen (p < 0.05). These low levels in the hospital compared to homes were attributed to the vigorous cleaning schedule in the hospital, the use of plastic to encased mattresses and pillows, vinyl covered sofas and vinyl lined floors. These practices may be adopted in the home as a means to reduce mite allergen exposure.


Assuntos
Ar Condicionado , Alérgenos/análise , Animais , Antígenos/análise , Antígenos de Dermatophagoides , Roupas de Cama, Mesa e Banho , Poeira , Ensaio de Imunoadsorção Enzimática , Pisos e Cobertura de Pisos , Glicoproteínas/análise , Hospitais Universitários , Decoração de Interiores e Mobiliário , Ácaros/imunologia , Singapura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA