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Objective@#To observe the effect of angiotensin ( 1-7) ［Ang( 1-7) ］on the apoptosis and angiogenesis of fibroblasts in the oral submucosal fibrosis ( OSF) ，and to explore the effect preliminarily mechanism．@*Methods@#Fibroblasts were isolated and cultured from human buccal mucosal tissue，the cell morphology was observed by inverted microscope，and the expression of vimentin was detected by immunofluorescence staining ; areca nut extract (ANE) was used to induce human fibroblasts to simulate the in vitro model of fibroblasts in OSF，the experimental groups included control group ( normally cultured cells) ，ANE group ( 100 μg/ ml ANE cultured cells for 48 hours) ，ANE + low-dose Ang( 1-7) group ( 100 μg/ ml ANE + 10－7 mol /L Ang ( 1-7) cultured cells for 48 h) ， ANE + high-dose Ang( 1-7) group ( 100 μg/ ml ANE + 10－5 mol /L Ang( 1-7) cultured cells for 48 h) ，immunofluorescence staining detected the expression of α-smooth muscle actin ( α-SMA) ，ELISA method detected the content of Collagen I and Collagen Ⅲ in the cell culture supernatant，MTT method detected cell proliferation activity， flow cytometry detected cell apoptosis ，the tubule formation experiment detected the vascularization of human umbilical vein endothelial cell(HUVEC) ; After the mRFP-GFP-LC3 virus was transferred to the cells，the level of autophagy was detected by immunofluorescence staining，Western blot detected the expression of autophagy-related protein Beclin-1 and the ratio of LC3-Ⅱ/ LC3-Ⅰ . @*Results@#The isolated and cultured cells were in a long spindle shape，and Vimentin was positively expressed，indicating that fibroblasts were successfully isolated ; Compared with the ANE group，the fluorescence expression of α-SMA protein in ANE low dose Ang( 1-7) group and ANE + high dose Ang( 1-7) group significantly decreased，the contents of Collagen I and Collagen Ⅲ in the culture supernatant were reduced (P＜0．05) ，cell proliferation activity decreased (P＜0．05) ，and cell apoptosis rate increased (P < 0．05) ，the cell culture supernatants of the two groups inhibited the angiogenesis of HUVEC (P＜0．05) ，endophagosomes were reduced (P＜0．05) ，Beclin-1 protein expression was reduced (P ＜0．05) ，and the ratio of LC3- Ⅱ / LC3-Ⅰ was down-regulated (P ＜0．05 ) ; in addition ，the effect of ANE + high-dose Ang ( 1-7 ) group was stronger than that of ANE + low-dose Ang( 1-7) group (P＜0．05) ．@*Conclusion@# Ang( 1-7) can inhibit the activation of fibroblasts induced by ANE，promote cell apoptosis，and reduce the angiogenesis of HUVEC，this mechanism may be related to the regulation of cell autophagy.
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Objective:To observe the effects of sonicated extract of Porphyromonas endodontalis(P.endodontalis)on the cell cy-cle and apoptosis of human osteoblastic hFOB 1 .1 9 cells.Methods:hFOB 1 .1 9 cells were treated with sonicated extract of P.end-odontalis at 0 (the control),1 ,1 0 and 1 00 μg/ml for 1 2,24 and 48 h respectively.MTT assay was used to examine cell prolifera-tion.The cell cycle distribution and apoptosis were examined by flow cytometry.Results:The sonicated exact of P.endodontalis in-hibited the proliferation of hFOB 1 .1 9 cells in a time-and dose-dependent manner.24 h treatment of 1 0 μg/ml and 1 00 μg/ml ex-act arrested the cell cycle of the cells in G1 phase,48 h treatment induced apoptosis in a dose-dependent manner.Conclusion:Sonicated exact of P.endodontalis may arrest hFOB 1 .1 9 cells in G1 phase and induce cell apoptosis,leading to inhibition of the cell proliferation.