Effects of paradoxical sleep deprivation on the ability oflearning & memory and gene expression in hippocampus in rats / 中国药理学通报

Aim To investigate the effect of 96 h paradoxical sleep deprivation(PSD) on learning & memory ability of rats and the concomitant gene expression change in the rat hippocampus.Methods PSD model was established by Modified Multiple Platform Method(MMPM).Learning and memory ability of the PSD model was assessed through hexagonal maze compared with the control, and the gene expression in hippocampus of each group was detected by gene chip technology.After that, GO and KEGG enrichment analysis were applied to determine the changes of the gene expression in PSD model compared with the control.Results PSD affected the memory consolidation process in rats.The gene chips showed that 100 genes expressed differently in the hippocampus of PSD model compared with the control, and among them, 25 genes were up-regulated and 75 genes down-regulated.The up-regulated genes mainly involved in cell growth and differentiation, learning and memory, inflammation, oxidative stress, and biological processes such as synaptic transmission, while the down-regulated genes mainly involved in immune response, central nervous system development, synaptic transmission, metabolism, signal transduction, neurotransmitter secretion, cell apoptosis, and so on.KEGG analysis showed that genes differentially expressed mainly involved in cGMP-PKG, metabolism, NF-kappa B and Pathways in cancer, and so on.Conclusion In rats, PSD leads to memory deficit, as well as a variety of gene expression changes in hippocampus of the PSD model.

Effect of Salidroside on the sleep phase of insomnia rats / 医学研究生学报

Objective:To observe the effect of the salidroside on the sleep phase of insomnia rats. Methods: Under the conditions of constant temperature,constant humidity,light automatically and electromagnetic shielding,the effect of the salidroside on the sleep phase of insomnia rats was observed with the method of the cortical EEG.Results:The results showed that salidroside can obviously decrease the duration of W and increase TST,primarily prolong SWS2 and REMS.Conclusion:Salidroside can prolong SWS2 and REMS of insomnian rats and regulate the sleep phase of insomnia rats near the normal rats.