Effects of Panax japonicus hypolipidemic compound on non-alcoholic fatty liver disease in mice and its mechanism / 解放军医学杂志

Objective To investigate the effects of Panax japonicas hypolipidemic compound (ZDS) on the lipid metabolism and its possible mechanism in non-alcoholic fatty liver disease (NAFLD) mice induced by high sugar and fat diet.Methods The extracts of Panaax japonica rhizoma,Salviae Miltiorrhiz radix Et rhizoma and Crataegi Fructus were prepared,and ZDS compound was formulated according to their antioxidant activities.Forty SPF male Kunming mice were randomly divided into four groups (10 each):normal control group,model group,high-dose ZDS-treated group,and low-dose ZDS-treated group.In addition to the mice in normal control group were given conventional diet,the mice in other three groups were fed high-sugar high-fat diet.High-dose and low-dose ZDS-treated group were given 90mg/kg or 30mg/kg ZDS.After the treatment of five weeks,the histomorphology and lipid deposition of the liver were observed to confirm the establishment of mouse NAFLD model and the improvement of ZDS compound on lipid deposition.The relative expression of miR-34a,SIRT1,and lipid metabolism related genes (FASN,ACC1) was detected by RT-qPCR and RT-PCR.SIRT1 protein expression was detected by Western blotting.Results Compared with the normal group,the morphological results showed hepatic lipid accumulation in the model group was more serious,the levels of triglyceride (TG) and miR-34a in the liver tissue increased significantly (P＜0.05),the expression levels of SIRT1 decreased,and the gene of lipid metabolism such as FASN,ACC1 significantly increased (P＜0.05).However,compared with the model group,ZDS compound improve hepatic lipid accumulation,liver TG content significantly decreased (P＜0.05),liver tissue miR-34a,FASN and ACC1 expressions decreased,while SIRT1 expression increased (P＜0.05).The protein expression of SIRT1 was consistent with its mRNA expression.Conclusion ZDS compound can effectively improve liver cell steatosis through the miR-34a/SIRT 1 pathway involved in lipid metabolism regulation,thus providing a new idea for early intervention of NAFLD through traditional Chinese compound medicine.

Long non-coding RNA HOTAIR in plasma as a potential biomarker for breast cancer diagnosis / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the expression of long non-coding RNA HOTAIR in the plasma of breast cancer patients and its value in the diagnosis of breast cancer.</p><p><b>METHODS</b>HOTAIR levels were measured in 24 tumor tissues and 70 plasma samples from breast cancer patients using quantitative real-time PCR. The correlations of plasma HOTAIR level with the clinicopathological features of the patients were analyzed. A multivariate logistic regression model was established to analyze the value of plasma HOTAIR in comparison with plasma CA153 and CEA levels for breast cancer diagnosis. We further detected HOTAIR levels in the plasma and breast cancer tissues of 24 patients before and after operation and investigated their correlation.</p><p><b>RESULTS</b>Breast cancer patients had increased expressions of HOTAIR in the tumor tissues and plasma, and plasma HOTAIR level was significantly correlated with estrogen receptor (ER) level (P=0.004) and lymph node metastasis (P=0.010). Receiver operating characteristic (ROC) curve and the multivariable logistic regression model showed that the area under ROC curve (AUC) of plasma HOTAIR was 0.82 (P<0.001) for breast cancer diagnosis with a diagnostic sensitivity and a specificity of 73.3% and 93.3%, respectively. The diagnostic power and specificity of plasma HOTAIR was much higher than those of CA153 (AUC=0.66, P=0.030) and CEA (AUC=0.52, P=0.001), and the combination of the 3 markers further enhanced the diagnostic power (AUC=0.84) and specificity (96.7%). Plasma HOTAIR level was significantly reduced in the patients after the operation (P<0.0001) and showed a moderate correlation with its expression in tumor tissues (r=0.62, P<0.0001).</p><p><b>CONCLUSION</b>Plasma HOTAIR may serve as a potential biomarker for breast cancer diagnosis.</p>

Clinical analysis of immediate implant and implant-supported complete denture for 12 patients with severe periodontitis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate clinical results of implant-supported complete denture of 12 patients with severe chronic periodontitis.</p><p><b>METHODS</b>After systematically periodontic treatment and controlling the inflammation, 12 cases with severe chronic periodontitis were extracted the residual teeth and received immediate implant placement. The implant-supported complete dentures were finished at 5 or 6 months after operation. Restorative results were evaluated with clinical examination, radiological examination and chief complaints of the patients.</p><p><b>RESULTS</b>A total of 108 implants were placed into 20 dental arches, including 37 immediate implants. Average loading was for 3 years and all implants were stable. Progressive bone resorption was observed around two implants. The average bone resorption of other peri-implants was (1.33 +/- 0.10) mm. The implant survival rate was 98.1%. The immediate implant survival rate was 97.3%.</p><p><b>CONCLUSIONS</b>After periodontic treatment, the patients with severe chronic periodontitis could be restored using immediate implant placement and implant-supported complete denture. It can shorten the treatment period, reduce absorption of alveolar and obtain a favorable result by oral health care.</p>