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Objective: To explore the effect of propaganda and training intervention about the ionization radiation knowledge for the cognition of these knowledge of elementary health care staff.Methods: A questionnaire survey about ionizing radiation knowledge was conducted on 181 medical workers in 10 township health hospitals in Xi'an.The contents of questionnaire included 5 parts that were basic knowledge,clinical application,daily protection,radiation hazard and emergency treatment.The Likert 3 grade scoring method was used to score the situation.And the training about ionizing radiation knowledge of protection was implemented for medical staff.The second questionnaires and scores were conducted after the intervention.And then the results of questionnaire survey and score between pre and post training were compared.Results: Before the training intervention,the average score of overall cognitive level of the ionizing radiation knowledge was(28.09±13.17),and the average score was increased to(49.79±10.35)after the training intervention.The difference of the number of three score segments(51-60,41-50 and 31-40)about the overall cognition of ionization radiation knowledge between pre and post interventions of propaganda and training were significant(x2=119.795,P＜0.05).The differences of clinical application score and basic knowledge between pre and post interventions of propaganda and training were no significant.And the scores of daily protection,radiation hazard and emergency treatment were increased to(11.39±0.35),(10.62±0.78)and(8.65±1.22)respectively,and the differences of them between pre and post interventions of propaganda and training were significant(t=2.18,t=1.87,t=1.56,P＜0.05),respectively.Conclusion: The cognitive interventions measurement of propaganda and training about preventive knowledge of ionization radiation can significantly improve the preventive awareness of elementary health care staff for ionizing radiation,and has a certainly promotion for enhancing the awareness and behavior of daily protection.
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The quality uniformity of traditional Chinese medicine (TCM) preparation is the base for guaranteeing the safety and effectiveness of clinical medication. At present, the quality of TCM preparation is uneven. At present, the same TCM preparation in different manufacturers, TCM preparations in the same manufacturer, and even different batches of a same TCM preparation in the same manufacturer have great differences in quality, which can not reach stability and uniformity. This paper would discuss the possible factors that influence the uniformity of quality in the whole process of pharmacy by means of consulting relevant literature on quality control of Chinese herbal preparations and analyzing the present situation and problems of the quality of TCM preparation. In addition, some strategies such as standardization of cultivation of TCM, processing standardization, standardization of pharmaceutical equipment, mixed batch feeding, and Quality by Design would be also put forward to provide references for the quality uniformity of TCM preparation.
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<p><b>OBJECTIVE</b>To investigate the effect of RYBP gene on sensitivity of HL-60 cells to chemotherapy drugs by using RNA interference.</p><p><b>METHODS</b>Plasmid expressing RYBP specific shRNA was constructed and then was used to establish the RYBP knockdown stable HL-60 cell line. Q-PCR and Western blot were used to confirm the efficacy of RYBP gene silencing at mRNA and protein level respectively; then the DNA ladder and Annexin V labeled flow cytometry were used to detect cell apoptosis; CCK-8 was used detect the sensitivity of HL-60 cells to the chemotherapeutic drug cytarabine or daunorubicin.</p><p><b>RESULTS</b>The lentiviral-RYBP-shRNA vector was succesfully and effectively inhibit the expression of RYBP at mRNA and protein in HL-60 cells. It was found that without chemotherapy drug treatment the apoptosis rate of RYBP shRNA group was lower than that of the empty vector control group (NC group). When treated with cytarabine, the apoptosis rate and inhibitive rate of RYBP shRNA group were lower than those of NC group. Besides, when treated with daunorubicin, the apoptosis rate of RYBP shRNA group was lower than that of NC group, while the inhibitive rate had no significant difference.</p><p><b>CONCLUSIONS</b>RYBP gene silencing can inhibitive the apoptosis of HL-60 cells and significantly reduce the sensitivity to cytarabine, but this gene silencing can't affect the sensitivity to daunorubicin.</p>
Assuntos
Humanos , Apoptose , Vetores Genéticos , Células HL-60 , Peptídeos e Proteínas de Sinalização Intracelular , Lentivirus , Plasmídeos , Interferência de RNA , RNA Mensageiro , RNA Interferente PequenoRESUMO
<p><b>OBJECTIVE</b>To study the effect of electromagnetic pulse (EMP) exposure on the permeability of blood-testicle barrier (BTB) in mice.</p><p><b>METHODS</b>Adult male BALB/c mice were exposed to EMP at 200 kV/m for 200 pulses with 2 seconds interval. The mice were injected with 2% Evans Blue solution through caudal vein at different time points after exposure, and the permeability of BTB was monitored using a fluorescence microscope. The testis sample for the transmission electron microscopy was prepared at 2 h after EMP exposure. The permeability of BTB in mice was observed by using Evans Blue tracer and lanthanum nitrate tracer.</p><p><b>RESULTS</b>After exposure, cloudy Evans Blue was found in the testicle convoluted seminiferous tubule of mice. Lanthanum nitrate was observed not only between testicle spermatogonia near seminiferous tubule wall and sertoli cells, but also between sertoli cells and primary spermatocyte or secondary spermatocyte. In contrast, lanthanum nitrate in control group was only found in the testicle sertoli cells between seminiferous tubule and near seminiferous tubule wall.</p><p><b>CONCLUSION</b>EMP exposure could increase the permeability of BTB in the mice.</p>