Study on Dinggui gel paste on analgesic and anti-inflammatory effects / 中国骨伤

<p><b>OBJECTIVE</b>To observe effects and mechanism of Dinggui gel paste analgesic anti-inflammatory.</p><p><b>METHODS</b>Eighty-four male KM mice weighted from 18 to 22 g and aged 4 to 5 weeks were randomly divided into 7 groups, named blank group, model group, matrix control group, Votalin group, high dosage of Dinggui gel paste group with group, equivalent dosage of Dinggui gel paste group, Dinggui gel paste group, 12 mice in each group. Except blank and model group, the other groups were paste ointment for 7 days, and one time a day, matrix control group were pasted isodose blank matrix gel patch. Pain threshold were tested at 30, 60, 90 and 120 min after the last ad-ministration. Hot plate test were performed by injection of 5% formalin for 20 µL on right hindfoot sole after the last administration. The cumulative time of mice licking right rear foot were observed at stage of I and II, and content of IL-1, TNF-α were tested by ELISA method. Differences of weight between right and left ears were measured by ear swelling method and anti-inflammation experiment.</p><p><b>RESULTS</b>In hot plate test at 90 min, pain threshold in equivalent dosage of Dinggui gel paste group was (24.87 ± 14.67) s and (15.28 ± 8.23) s in model group; (26.33 ± 15.45) s in high dosage of Dinggui gel paste group and (15.31 ± 5.02) s in model group at 120 min in hot plate test, there were no statistical differences between two groups. Pain period at stage I, licking cumulative time in high dosage of Dinggui gel paste group was (66.70 ± 22.83) s and (101.80 ± 33.65) s in model group,and had significant differences between two groups; there were statistical differences in licking cumulative time at stage I of pain period among high dosage of Dinggui gel paste group (51.30 ± 43.60)s, equivalent dosage of Dinggui gel paste group (64.00 ± 47.27) sand model group (109.50 ± 36.78) s. Content of IL-1 in model group was (28.70 ± 8.24) ng/L and (13.33 ± 2.20) ng/L in high dosage of Dinggui gel paste group, there was obvious meaning between two groups; There were significant differences in TNF-α content among model group (93.60 ± 23.65) ng/L,high dosage of Dinggui gel paste group (63.21 ± 10.54)ng/L and equivalent dosage of Dinggui gel paste group (72.69 ± 16.26) ng/L; while there were no statistical meaning in ear swelling degree among model group (5.73 ± 0.80) mg,high dosage of Dinggui gel paste group (5.42 ± 0.68) mg and equivalent dosage of Dinggui gel paste group (4.98 ± 1.52) mg.</p><p><b>CONCLUSION</b>Dinggui gel paste could increase pain threshold, reduce licking accumulative time, and decrease ear swelling degree, and relief pain by regulating level of TNF-α and IL-1.</p>

Comparison on release and transdermal behavior in vitro between Dinggui Gel Paste and Dinggui Huoluo Rubber Patch / 中草药

Objective: To compare the release and transdermal behavior in vitro between Dinggui Huoluo Rubber Patch (DHRP) and Dinggui Gel Paste (DGP) which is the improved form. Methods: Microporous filter membrane or Strat-M membrane and advanced Franz diffusion cell were chosen in the release and transdermal experiment in vitro. The content of notopterol, the active component of the key herb Notopterygium incisum in the preparation, was quantitatively determined by HPLC method to determine the release rate, penetration quantity, and penetration rate of the two forms. Results: The penetration formula of DGP was Ln = 9.208 t-18.50, r = 0.948. The in vitro cumulative release rate, penetration quantity, and penetration rate were respectively 85.34%, 0.661 8 mg/g, and 65.67%. The penetration formula of DHRP was Ln = 4.285 t-6.704, r = 0.977. The in vitro cumulative release rate, penetration quantity, and penetration rate were respectively 67.87%, 0.181 6 mg/g, and 31.35%. Conclusion: The transdermal behavior of DGP is obviously better than that of DHRP while its quantity and the rate of penetration and release are all better than those of rubber patch. Meanwhile the gel paste is less sensitized and more convenient to use.