RESUMO
Objective:To investigate the effect of vasoactive intestinal peptide on IL-1β expression after corneal alkali burn.Methods:27 healthy adult female Wistar rats were randomly divided into group A(normal blank control group),group B(alkali burn treated with saline group),and group C (alkali burn treated with vasoactive intestinal peptide group).Group A were not processed.Group B,C were made right eyes′moderate corneal alkali burn models.After inducing burn,group B and group C were followed respectively by 0.9% saline and 0.25 g/L vasoactive intestinal peptide eye drops,4 times per day.The corneal epithelial healing rates were calculated by measuring the corneal staining area by methylene blue staining at 3 d,7 d and 14 d after inducing burn.At 3 d,7 d and 21 d,the corneal tissues were removed and divided into two equal parts.The corneal tissues were observed by staining,and the expression of IL-1β were detected by immunohistochemistry.Results:The healing rates of corneal epithelium in group C were greater than those in group B at 7 d and 14 d(P<0.05).The mean optical density of IL-1β was measured by immunohistochem-istry.The mean optical density in group B and group C at 3 d were(1.62 ± 0.96) ×10-2,(0.98 ± 0.45) ×10-2,respectively,which at 7 d were (1.42 ± 0.62) ×10-2,(0.71 ± 0.22) ×10-2,respectively,which at 21 d were(0.37 ± 0.24) ×10-2,(0.13 ± 0.16) × 10-2,respectively.The mean optical density of corneal IL-1β in group C were lower than those in group B(P<0.05).Conclusion:Va-soactive intestinal peptide can decrease the expression of IL-1β after corneal alkali burn,down-regulate the level of immune stress, reduce the inflammatory reaction and promote the recovery of cornea after alkali burn.
RESUMO
Anthocyanin from purple sweet potato (PSP) extracted by microwave baking (MB) and acidified electrolyzed water (AEW) exhibited antioxidant activity. After further purification by macroporous AB-8 resin, the color value of PSP anthocyanin (PSPA) reached 30.15 with a total flavonoid concentration of 932.5 mg/g. The purified extracts had more potent antioxidant activities than the crude extracts. After continuously administering the PSP extracts to 12-mo-old mice for 1 mo, the anti-aging index of the experimental group was not significantly different from that of 5-mo-old mice. To a certain degree, PSPA was also effective for controlling plasma glucose levels in male Streptozocin (STZ)-treated diabetic mice. In addition, the extracts inhibited Sarcoma S180 cell growth in ICR mice. Mice consuming the PSP extracts formed significantly fewer and smaller sarcomas than mice consuming the control diets. The highest inhibition rate was 69.03%. These results suggest that anthocyanin extracts from PSP not only exert strong antioxidant effects in vitro, but also had anti-aging, anti-hyperglycemic, and anti-tumor activities.