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1.
Journal of Southern Medical University ; (12): 1165-1167, 2008.
Artigo em Chinês | WPRIM | ID: wpr-270185

RESUMO

<p><b>OBJECTIVE</b>To investigate osteopontin (OPN) mRNA expression in oral squamous cell carcinoma (OSCC) and normal oral mucosa tissues.</p><p><b>METHODS</b>Differential OPN gene expression were detected in 30 cancerous tissues and their paired normal tissues using real-time reverse transcription-PCR (real-time RT-PCR), and the data were analyzed statistically.</p><p><b>RESULTS</b>Real-time RT-PCR results demonstrated that the relative expression level of OPN mRNA in the cancerous tissues were significantly higher than that in paired normal samples (4.17-/+0.51 vs 0.97-/+0.12, P<0.001), showing a 4.3-fold up-regulation. In the 30 OSCC specimens, OPN mRNA expression in the OSCC of histological grades I showed a 3.1-fold down-regulation, significantly lower than the expression in grade II/III tumors (2.16-/+0.17 vs 6.80-/+0.72, P<0.05); its expression was significantly lower in early stage than in advanced stage OSCCs (2.34-/+0.17 vs 4.73-/+0.35, P<0.05). In cases of cervical lymph node metastasis, the expression was significantly higher than that in cases without lymphatic metastasis (6.38-/+0.56 vs 2.89-/+0.32, P<0.05).</p><p><b>CONCLUSION</b>OPN mRNA overexpression may play an important role in OSCC carcinogenesis and can be a potential target for OSCC therapy.</p>


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma de Células Escamosas , Genética , Patologia , Regulação Neoplásica da Expressão Gênica , Metástase Linfática , Mucosa Bucal , Metabolismo , Patologia , Neoplasias Bucais , Genética , Patologia , Osteopontina , Genética , RNA Mensageiro , Genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa
2.
Chinese Journal of Stomatology ; (12): 456-460, 2006.
Artigo em Chinês | WPRIM | ID: wpr-354345

RESUMO

<p><b>OBJECTIVE</b>To select and identify the target genes related to oral squamous cell carcinoma (OSCC) and provide target genes for designing oligo-nucleotide functional microarray of OSCC.</p><p><b>METHODS</b>Genes possibly related to oral squamous cell carcinoma were selected from the 5 years' published data of differently expressed profiles with microarray testing in OSCC. Then mRNA expression of selected genes were evaluated by real time quantitative polymerase chain reaction (RT-PCR) in 22 cases of OSCC, including tumor tissues and paried normal mucosas and quantified according to an internal control GAPDH.</p><p><b>RESULTS</b>Eight genes were tested. The overexpression of SPARC, PDGF-A, SERPINE1, TGF-beta(1) and VEGF-C genes were measured in 16, 18, 16, 20, 18 cases of tumor specimens, respectively. The expression of CK15 gene was lower than that of its normal tissue. There were overexpression of CCND1, BIRC3 in tumor tissues, but there was no significant difference of CCND1 and BIRC3 expression between tumor tissue and normal tissue (P > 0.05).</p><p><b>CONCLUSIONS</b>SPARC, PDGF-A, SERPINE1, TGF-beta(1), VEGF-C and CK15 genes were closely related to tumor progress of OSCC. They can be used as the target genes for designing oligo-nucleotide functional microarray of OSCC.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Biomarcadores Tumorais , Genética , Carcinoma de Células Escamosas , Genética , Neoplasias Bucais , Genética , Análise de Sequência com Séries de Oligonucleotídeos , Métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Métodos
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