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@#【Objective】To evaluate the efficacy of early filtering surgery in newborns with primary congenital glaucoma（NPCG）.【Methods】A total of 70 eyes of 39 patients with NPCG，who underwent trabeculotomy or combined trabeculotomy-trabeculectomy within 3 years old were retrospectively studied. Preoperative and postoperative intraocular pressure（IOP），corneal clarity and diameter，optic disc cupping and visual acuity were evaluated. Kaplan-Meier survival analysis of the success rates were studied. Success rates of the patients who underwent surgery within 1 month old（22 eyes）and older than 1 month old（48 eyes）were compared by Log- Rank.【Results】The postoperative IOP（mmHg；16.9 ± 5.2） were significantly lower than the preoperative IOP（28.7±5.8 ，P<0.001）. At the last visit ，the cornea became clearer ， C/D（cup/disc）ratio decreased significantly，while the horizontal corneal diameter did not change significantly in all the children. The success rates for all eyes at 1，2，3，6 and 9 years after operation were 94.3%（63 eyes），90.6%（52 eyes），85.9%（40 eyes），85.9%（23 eyes），and 85.9%（15 eyes），respectively. The success rates and visual acuity at last visit of patients who underwent surgery within 1 month old were better than those older than 1 month old（P=0.033； P=0.01）. There were no severe intraoperative or postoperative complications.【Conclusions】Early surgery such as trabeculotomy or combined trabeculotomy-trabeculectomy is a safe and effective treatment for NPCG.
RESUMO
Objective To investigate the reversal effect of MDR1 and MDR3 gene silencing on resistance of A2780/taxol cells to paclitaxel.Methods shRNA plasmid vector specifically targeting MDR1 and MDR3 genes was transfected into A2780/taxol cells.The early stage cell apoptosis and the effect of intracellular rhodamine 123(Rh123)accumulation were detected by flow cytometry(FCM).The late stage cell apoptosis rate was detected by terminal deoxynucleotidyl transferase(TdT)-mediated deoxyuridine triphosphate(dUTP)nick end labeling(TUNEL).The 50% inhibition concentration(IC_(50))of paclitaxel on A2780/taxol cells was determined by methyl thiazolyl tetrazolium(MTT)assay.MDR1 and MDR3 mRNA were assessed by RT-PCR,and caspase-3 protein was detected by western blot.Results After treatment with MDR1 and MDR3 shRNA plasmid vector,early apoptosis rate of A2780/taxol cells was (20.21?0.56)% and(10.87?1.24)%,respectively.MDR1 and MDR3 shRNA could increase cellular Rh123 accumulation(116.6?8.1 and 98.4?3.8,respectively).The late stage apoptosis rates detected by TUNEL displayed the same tendency as FCM results did.The IC_(50)for paclitaxel of A2780/taxol cells was decreased significantly.The mRNA levels of MDR1 and MDR3 in A2780/taxol cells were decreased by (73.3?0.8)% and(51.6?0.4)% of control,and the reduction of MDR1 and MDR3 mRNA was in a time-dependent manner.The expression of caspase-3 protein of MDR1 and MDR3 shRNA vector transfected group in A2780/taxol cells was significantly increased [(80.8?2.6)% and(72.0?4.7)%, respectively ].Conclusion MDR1 and MDR3 gene silencing could recover sensitivity of A2780/taxol cells to paclitaxel and induce cell apoptosis,thus reversing cell resistance to paclitaxel.