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Objective@#To illuminate the epidemic characteristics of Yersiniosis among children in the central city of Beijing and the accuracy of current clinical diagnosis towards Yersiniosis.@*Methods@#Etiological surveillance of diarrheal patients, a total of 3 493 cases, was performed in a children hospital in central area of Beijing from 2011 to 2018 continuously. Collected the epidemiological and clinical information of the cases, analyzed the clinical and etiological diagnosis for Yersiniosis and bacterial dysentery and compared the distribution of Yersiniosis cases with the different symptoms.@*Results@#A total of 3 493 acute diarrhea cases distributed from the age of 6 months to 13 years old, M (P25, P75) was 1.50 (0.75, 3.17) years old. The 28 cases were isolated Yersinia enterocolitica (isolation rate of 0.80%) and they could be diagnosed as Yersiniosis by etiology. The isolation peaked in May and February. A total of 85.71% (24/28) of Yersiniosis cases were under 5-year old. The children of 3-4 age group had the highest isolation rate (1.52%) while the rate (0.18%) of 0-1 age group was the lowest (P=0.025). The Yersinia enteroclitica isolation rates of diarrheal patients with the symptoms including mucus feces, fever, white blood cell (WBC) and red blood cell (RBC) in feces were higher than the patients without these symptoms (P>0.05). The 9 of 28 Yersiniosis cases by etiology diagnosis were clinical diagnosed as bacillary dysentery.@*Conclusion@#The infants and young children under 5-year old were the main population of Yersiniosis adolescent patients under 14-year old. The typical symptoms characterized with mucus stool, fever, WBC and RBC by routine microscopic examination. The preliminary clinical diagnosis of Yersiniosis is easily confused with bacterial dysentery.
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Objective@#To analyze the VP1 genetic sequences of Enterovirus A group 71 (EV-A71) strains and Coxsackievirus A group 16 (CV-A16) strains from suspected hand, foot, and mouth disease(HFMD) cases in Dongcheng District, Beijing in 2017, in order to provide basic data for the prevention and control of HFMD.@*Methods@#By RT-PCR, the VP1 sequences of EV-A71 and CV-A16 VP1 were amplified, and those sequences were compared and analyzed with DNAstar 5.0, while the phylogenetic tree was constructed with MEGA 6.0 software.@*Results@#Totally 95 throat swabs of suspected HFMD cases were detected in Dongcheng District, 2017. Forty-six (48.42%) specimens were positive for enterovirus, 4 (4.21%) specimens were positive for EV-A71 and 4 (4.21%) specimens were positive for CV-A16, 38 (40.00%) specimens were positive for non-EV-A71, non-CV-A16 enterovirus. The nucleotide and amino acid sequence homology of the four EV-A71 strains were respectively 92.89%-99.15% and 97.32%-98.66%. The nucleotide and amino acid sequence homology of the three CV-A16 strains were respectively 93.90%-98.76% and 98.32%-100.00%. The phylogenetic tree indicated that EV-A71 strains belonged to C4a subtype and CV-A16 strains belonged to B1b subtype.@*Conclusions@#The main pathogens causing HFMD were non-EV-A71 and non-CV-A16 enteroviruses in Dongcheng District of Beijing in 2017, but EV-A71 and CV-A16 still account for a certain proportion. The EV-A71 strains belong to C4a genetic subtype; the CV-A16 strains belong to B1b genetic subtype. The subtypes of gene matched with the dominant strains in most areas of China in recent years and no new subtypes were detected.
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Objective@#To analyze the etiological characteristics of infectious diarrhea among people under 5 years old in Dongcheng District, Beijing.@*Methods@#The age, time of infection, clinical symptoms and laboratory test results of the cases who didn't used antibiotics within 3 days in the second maternal and child health care hospital were collected from 2012 to 2015, through the information management system of infectious disease monitoring technology platform. To compare the detection rate of virus and bacteria in children with different sex, time and age,and the difference of clinical characteristics between virus detection group and bacteria detection group by chi square test.@*Results@#1 977 cases of infectious diarrhea were collected, the median of the month age (P25, P75) was 14.19 (8.31, 23.15) months. The virus detection rate was 34.3% (679 cases); the bacterial detection rate was 14.6% (288 cases). The difference of virus detection rate in children with different months was statistically significant (χ2=72.38, P<0.001), the virus detection rate of 24-60 months (40.9% (188/460)) was the hightest, and the detection rate of 0-5 months (15.3% (48/314)) was the lowest. The difference of bacteria detection rate was also statistically significant (χ2=32.67, P<0.001), and the detection rate of 12-17 months (19.0% (81/426)) was the highest, the detection rate of 0-5 months (6.7% (21/314)) was the lowest. The proportion of vomit and water sample in the virus detection group was 22.2% (136 cases) and 73.3% (449 cases), respectively, which were higher than those in bacteria detection group (8.1% (18 cases) and 57.2% (127 cases)), the difference was statistically significant (χ2 values were 125.92 and 19.60; P values were both<0.001); the proportion of mucus stool and fever was 0.8% (5 cases) and 14.0% (86 cases), respectively, which were lower than those in bacterial detection group (4.1% (9 cases) and 18.5% (41 cases)), and the difference was statistically significant (χ2 values were 8.50 and 23.01; P values were 0.004 and <0.001).@*Conclusion@#The virus detection rate of infantile infective diarrhea is higher than that of bacteria in Dongcheng district of Beijing, and the clinical characteristics are significantly different.
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Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared from the roots of T. hypoglaucum amd its antiviral activity against HSV-1 in Vero cells was evaluated by cytopathic effect (CPE) assay, plaque reduction assay and by RT-PCR analysis. The alkaloids extract presented low cytotoxicity (CC_(50) = 46.6 μg/mL) and potent CPE inhibition activity, the 50% inhibitory concentration (IC_(50)) was 6.5 μg/mL, noticeably lower than that of Acyclovir (15.4 μg /mL). Plaque formation was significantly reduced by the alkaloids extract at concentrations of 6.25 μg/mL to 12.5 μg/mL, the plaque reduction ratio reached 55% to 75% which was 35% higher than that of Acyclovir at the same concentration. RT-PCR analysis showed that, the transcription of two important delayed early genes UL30 and UL39, and a late gene US6 of HSV-1 genome all were suppressed by the alkaloids extract, the expression inhibiting efficacy compared to the control was 74.6% (UL30), 70.9% (UL39) and 62.6% (US6) respectively at the working concentration of 12.5μg/mL. The above results suggest a potent anti-HSV-1 activity of the alkaloids extract in vitro.
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Objective MMP-9 and CD44 together position in the cell surface,and this kind of together positions to help the CD44 vto settle anchor roans MMP-9s to also decline a solution to the gum original IV at the cell surface and promote tumor cell gradually. Stopping the empress of the function of CD44 with the dissolubility CD44 can immediately make it lose the function that the anchor man series MMP-9. But is settle by the CD44 anchor manses superficial at the cell of the MMP-9 can also make TGF-β1 of have no activity-TGF-β1 ex- bodies activate and activate of TGF-β1 new-born blood vessel growth is the essential growth factor. This text passes the MMP-9 and TGF-β1 which settles to the CD44 anchor manses-TGF-β1 the expression circumstance carries on analysis in the brain star polygon ceil lump,as to it's express meaning to carry on a research,to the CD44,MMP-9,TGF-β1 gradually transfer amedium function to carry on a first step study in the brain star polygon cell lump,in the meantime to the CD44,MMP-9,TGF-β1 relativities carry on an analytical research. Methods Totally collect 2006 subsidiary hospital of Zheng4 Zhou's university first and Xu3 Chang hospital surgical operation in the city center excision of the brain star polygon coil lump the specimen be 98 and apply an immunity set to turn the SP method examination tumor the CD44,MMP-9,TGF-β1 in the organization-TGF-β1 expression,analytical CD44, MMP-9,TGF-β1 with and CD44, MMP-9,TGF-β1 of the brain star polygon cell lump ratings-TGF- 131. The relation of one its. Results (1)CD44 the expression rote is 77.55%(76/98) in the star polygon cell lump organization and show Zhao Gao to organize 0 at the normal brain (P<0.05). Pass the related examination of the Spearman grade,different Class CD44 expression r=0.95,P=0.046, have covariance to learn meaning,the of the star polygon ceil lump Class and the CD44 male degree-present just related. Use the Fishers precision examination method to carry on an examination to the different Claas,P=0.00027,the composing ratio which explains an of different Class CD44 expressions contains difference. (2)MMP-9 the expression rate is 69.38%(68/98) in the star polygon cell lump organization and show Zhan Gan to organize at the normal brain (P< 0.05). Pass the related examination of the Spearman grade, different Class MMP-9 expression r=0.89,P=0.038,have eovariance to learn meaning.The of the star polygon ceil lump Class and the MMP-9 male degree present just related,the composing circumstance which uses the Fishers precision examination method 4 observation result of the Class MMP-9s to the tumor carries on an examination,P=1.12×10-7. (3)TGF-β1 the expression rate is 74.481%(73/98) in the star polygon ceil lump organization and show Zhao Gao to organize at the normal brain (P<0.05). Pass the related examination of the Spearman grade,different Class TGF-β1 expression r=0.91,P=0.046,have covariance to learn intoning. The star polygon cell lump Class and TGF-β1 of male degrees present just related.Use the Fishers precision examination method 4 Class TGF-β1 to the tumor-TGF-β1 composing circumstance of observation result carry on an examination,P=0.0001. (4)Star polygon cell CD44,MMP-9,TGF in lump-TGF-β1 expression of relativity analysis CD44 expression masculine gender of in the case,the male expression rate of MMP-9 is 94.12%,CD44 expression feminine gender of in the case,the feminine gender expression of the MMP-9 rate is 60.00%, of the expression and the expressions MMP-9s of CD44 presents a just related relation (the row contact few rp=0. 496016, P=1.559 × 10-8). The CD44 expresses a male case In,TGF-β1 male expression rates is 82.89%,in the ease of the CD44 expression feminine gender,TGF-β1 feminine gender expression rates is 54.55%, the expression and TGF-β1 of CD44-TGF-β1 expressions present a just related relation (the row contact few rp=0.3136, P = 0.001076). The MMP-9 expresses a male case in, TGF-β1 male expression rates is 91.17%,in the ease of the MMP-9 expression feminine gender,TGF-β1 feminine gender expression rates is 63.33%,Be learned analysis the enunciation MMP-9 to express by covariance and TGF-β1 expressions present a just related relation (the row contact few rp=0.4825,P=4.93 × 10-8). Conclusion The deteedion of MMP-9,CD44 and TGF-β1 is significance for prognosis of the brain star polygon cell lump.