Association between male infertility and either the +331G/A or the progins polymorphism of the progesterone receptor gene in a Chinese population

Progesterone has been suggested to contribute to the regulation of spermatogenesis and to facilitate the production of viable sperm. Investigations have showed that polymorphism of progesterone receptor [PGR] is associated with some diseases. To analyze the potential relationship between male infertility and the +331G/A and progins polymorphisms of PGR gene. The cross-sectional study was carried out at the Department of Male Reproduction, Reproductive Medical Center, the Second Hospital of Jilin University. The restriction fragment length polymorphism [RFLP] technique was used to detect gene point mutations. Of the 145 semen samples analyzed, 35 were asthenozoospermic, 50 were oligoasthenozoospermic, 21 were azoospermic, 11 were teratozoospermic and 28 were from fertile male subjects. Statistical analyses revealed that the genotypes of the +331G/A polymorphisms were in Hardy-Weinberg equilibrium in both the fertile [chi[2]=0, p=0.534] and oligospermic groups [chi[2]=0.021, p=0.537]. Similarly, the genotypes of the progins polymorphisms were also in Hardy-Weinberg equilibrium in both the fertile [chi[2]=0, p=1] and oligospermic groups [chi[2]=0.005, p=1]. Our results indicated that polymorphisms of the +331G/A and progins of the PGR gene are unrelated to male infertility, at least in a Chinese population

Best time of mesenchymal stem cells transplantation for treatment of myocardial infarction in aged rabbits / 吉林大学学报(医学版)

Objective To investigate the best time of the mesenchymal stem cells(MSCs) transplantation after myocardial infarction(MI),in order to provide prophase experimental basis for treatment of the diseases correlating to cadiocyte necrosis by cells transplantation.Methods Thirty New Zealand big-ears albino rabbits,which aging were older than or equal to 36 months were selected(a 36-month rabbit is the advanced age rabbit,equaling a person 60 years old).Model of MI was made by ligating anterior descending branch.The model rabbits were randomly divided into six groups(control group,1 d group,1-week group,2-week group,3-week group;and 4-week group,n=5).200 ?L cells after marked were drawed and injected in multiple positions of myocardium after MI in 1 d group,1-week group,2-week group,3-week group and 4-week group separately.The rabbits in control group were injected with saline of equal quantity.The cardiac functional parameters,blood flow rate of atrioventricular valve and the hemodynamics at eighth week in various groups were measured.Results Compared with control group,the treatment of MSCs transplantation for MI improved the heart function.The cardiac functional parameters,blood flow rate of atrioventricular valve and the hemodynamics in 1 d group was higher than those in control group(P0.05).The cardiac functional parameters,blood flow rate of atrioventricular valve and the hemodynamics in 1-week,2-weeks and 3-weeks groups were better than those in 1 d group(P0.05).Conclusion In the condition of the same cell-density but the different time of MI,the transplantation time window is 1 d-3 weeks after MI,which is advantageous to the amelioration of heart function,the best time of transplantation was 1-3 weeks after MI.

Surgical treatment of perforation of gastric carcinoma / 中国普通外科杂志

Objective To study the outcome of surgical therapy of perforation of gastric cancer. Methods The clinical data of 37 cases with perforation of gastric carcinoma which underwent different surgical procedures were analyzed retrospectively. Among them,24 cases underwent partial gastrectomy,8 cases underwent radical mastectomy,4 cases had repair of perforation. 1 case received non-operative therapy. Results The preoperative diagnotic rate was 24.3%,and misdiagonsis rate was 75.7%. The perioperative mortality rate was 5.4%,including 1 case that underwent repair of perforation. The post-operative complication rate of patients undergoing palliative gastrectomy,radical gastric cancer operation and repair of perforation was 41.7%,25.0% and 75.0%,respectively,and the mean survival time was 25 months,29 months and 3.0 months,respectively.The partial gastrectomy and radical mastectomy patients had significantly better outcomes than those who underwent repair of perforation or received non-operative therapy.Conclusions For patients with perforation of gastric cancer with resectable lesions and who are in good general coaditon,a one-stage radical gastric resection or palliative subtotal gastrectomy can be performed.