RESUMO
Objective To establish a method for the determination of aristolochic acid A inPaishi granule.Methods The HPLC system consisted of the Phenomenex Luna C18(4.6 mm×250 mm, 5μm)column, the mobile phase consisted of acetonitrile and 0.01% HAc, gradient elution flow rate was 1.0 ml/min, the column temperature was 35℃, The UV detector was set at 250 nm.Results The linear response range was 0.029-0.580μg/ml (r=0.999 9). The detection limit and quantitation limit of aristolochic acid A inPaishi granule were 0.9 and 3.0 ng/ml. The average recovery of aristolochic acid A was 96.4%.Conclusion The method is high sensitivity, accurate, repeatable and high specificity,and can be used as an inspection method for safe use of Paishi granule.
RESUMO
Objective To establish a HPLC method for the determination of Isorhamnetin3-O-nehesperidinein Shenghua Tablets.Methods The RP-HPLC system was consisted of the Luna-C18(4.6 mm×250 mm,5 μm) column,the mobile phase was acetonitrile -0.4％H3PO4 (10 ∶ 90),the flow rate was set at 0.8 ml/min,the column temperature was 30*℃,and the UV detector was 254 nm.Results The linear response range was 0.056～ 1.12 mg/ml (r=0.9997).The average recovery of mangiferin was 98.0％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets.