RESUMO
Objective To compare the advantages and disadvantages of the two methods.Methods Thirty BALB/c mice were randomly divided into 3 groups:normal control group,OVA group and OVA/RSV group.With ovalbumin and hydroxide suspension sensitized,OVA nouse asthma model group and OVA/RSV group were established respectively by continuous atomization of ovalbumin and repeated nasal drops of RSV.The control group was established by sensitization and atomization excitation of sterile injection water.24 hours after the last stimulation,the lung function was measured.The cell sorting and counting in bronchoalveolar lavage fluid were performed.The pathological changes of lung tissue were observed by HE staining.Results Compared with the control group,the model groups have obvious symptoms of asthma,airway wall thickening,lumen stenosis and a large number of inflammatory cell infiltration.And pathological damage of lung tissue in OVA/RSV group was significantly increased,compared with OVA group.The enhanced expiratory pause (Penh)values of OVA/RSV group were significantly higher than those in group OVA (P ＜ 0.05),and the number of neutrophils,monocytes,lymphocytes and eosinophils in BALF were significantly higher than those in OVA group (P ＜ 0.05).Conclusion The establishment of asthma mouse model by OVA sensitization and RSV induction is a better way to simulate the human asthma pathogenesis.
RESUMO
Objective To analyse effect of different dose of sustained-release theophyline on prealbumin, C-reactive protein and white blood cell count in patients with pediatric asthma.Methods 58 children with acute asthma attack in our hospital were collected.All children were randomly divided into high dose group and low dose group,29 cases in each group.On the basis of conventional treatment, high dose group were treated with high dose theophylline sustained release tablets 5 mg/kg, one times per eight hours,orally,and low dose group was treated with low dose theophylline sustained release tablets,2 mg/kg, one times per eight hours,orally.Two groups were treatment for two weeks.After the treatment, the serum levels of pre albumin, C-reactive protein and veinal blood level of white blood cell count were detected in all children.Results Compared with high dose group post-treatment, the serum level PA was higher in low dose group (P<0.05);the serum level of CRP was lower in low dose group (P<0.05);the veinal blood level of WBC, percentage of neutrophils and percentage of eosinophils was lower in low dose group (P<0.05) .Conclusion Compared with high dose of sustained-release theophyline,the low dose of sustained-release theophyline can significantly reduce the serum CRP level and veinal blood level of WBC, percentage of neutrophils and percentage of eosinophils, improve serum PA level in patients with pediatric asthma, reduce inflammation in patients.