RESUMO
BACKGROUND:Intervertebral disc degeneration is the basis of spinal degenerative diseases;however,there is no effective treatment. OBJECTIVE:To investigate whether sinomenine can inhibit interleukin-1β-induced apoptosis in nucleus pulposus cells and its molecular mechanism. METHODS:Rat nucleus pulposus cells were cultured in vitro by trypsin combined with type II collagenase digestion,and the cell growth curve was plotted.An appropriate sinomenine concentration was determined using the cell counting kit-8 kit.Nucleus pulposus cells were divided into control group,sinomenine group,interleukin-1β group,sinomenine+interleukin-1β group,zinc protoporphyrin group,zinc protoporphyrin+sinomenine group,zinc protoporphyrin+interleukin-1β group,and sinomenine+zinc protoporphyrin+interleukin-1β group.Proliferative activity,reactive oxygen species content,apoptosis rate,and heme oxygenase-1 expression in nucleus pulposus cells were detected. RESULTS AND CONCLUSION:The rat nucleus pulposus cells cultured in vitro were polygonal,triangular,and short wedge-shaped,and the cell growth showed an"S"curve.The cells grew slowly in the first 3 days of culture,rapidly in 4-6 days,and slowly again in 7-8 days.The cells then entered the"platform stage"where the number of cells no longer increased.The proliferative activity of myeloid cells showed no significant changes when the concentration of sinomenine was≤80 μmol/L(P>0.05).Interleukin-1β significantly reduced the proliferative activity of nucleus pulposus cells,increased the content of reactive oxygen species and led to apoptosis(P<0.01).Sinomenine intervention not only promoted heme oxygenase-1 expression(P<0.05)but also inhibited interleukin-1β-induced decrease in proliferative activity and increase in reactive oxygen species content and apoptosis rate in nucleus pulposus cells(P<0.05).These effects could be reversed by zinc protoporphyrin(P<0.01).
RESUMO
Objective To explore the influence of different biological sources of thromboplastin reagent on prothrombin time (PT) detection .Methods The thromboplastin reagents sourced from the rabbit brain extract provided by the Shanghai Taiyang Bi‐ological Technology Co .,Ltd .and Shanghai Changdao Biological Technology Co .,Ltd .and recombination tissue factor were selected and used for detecting PT in the non‐Warfarin treatment group(group A ,38 cases) and the Warfarin group(group B ,43 cases) by the Sysmex CA7000 blood coagulation analyzer .The detection results from different sources of prothrombin reagents were com‐pared between the two groups and performed the statistical analysis .Results In the group A ,the detection results had no statistical differences among the reagent R1 ,R2 ,R3 and R4 (F=3 .42 ,P>0 .05 ,n=38);in the group B ,the detection results had statistical differences among the reagent R1 ,R2 ,R3 and R4 (F=57 .68 ,P0 .05) and between reagent R2 and R4(t=2 .09 ,P>0 .05) .Conclusion The detec‐tion results of PT by different sources of PT reagent in the non‐Warfarin treatment patients demonstrate no significant difference , but the detection results in the Warfarin treatment patients have highly significant difference;the recombination tissue factor throm‐boplastin reagent has higher sensitivity ,which used for monitoring PT in the warfarin treated patients should establish a new thera‐peutic standard value for avoiding to cause the misdiagnosis and mistreatment .