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Objective:To summarize the major risk factors of infection in the process of cleaning and disinfection of endoscopy center in our hospital,and formulate the corresponding prevention measures to reduce the incidence of endoscopic infection so as to ensure medical safety of patients.Methods:The specialist check the cleaning disinfection process,statistics of each link in the risk factors of infection.600 endoscopes were taken at random after disinfection,endoscopic inner cavity and the outer surface samples are collected,the cleaning and disinfection of the test results for evaluating quality indicators.Results:Testing 600 samples of qualified 584,and the percent of pass is 97.3％,gastroscopy,colonoscopy,duodenum mirror,bronchoscope,endoscopic ultrasonography qualified rate was 98.6％,96.8％,95.6％,96.0％,97.2％,respectively.28 strains of pathogenic bacteria were detected in 16 cases of unqualified samples,including 13 strains of helicobacter pylori,7 strains of escherichia coli,4 strains of pseudomonas aeruginosa,2 strains of pseudomonas aeruginosa,1 strains of staphylococcus aureus,1 strains of Klebsiella pneumoniae;16 cases to detect unqualified reason analysis,scrub incomplete accounted for 37.5％,Not according to the instructions required to use multi-enzyme and disinfectant accounted for 18.75％,special infected patients did not do special treatment of endoscopy accounted for 12.5％,endoscopic insufficient drying and the staffhand hygiene is not up to standard accounted for 12.5％,respectively.Conclusion:Endoscopic cleaning and disinfection aspects of infection caused by many factors,infection control education should be strengthened for all personal in the endoscopy center,regular professional training of cleaning and sanitation personal,cleaning and disinfection process should be standardized management,establish traceability registration system,control the quality of the endoscope cleaning and disinfection from each link.It is the main measure to prevent digestive endoscopy center in infection.
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Objective To evaluate the inhibitory effect of endostatin on ovarian cancer cell line SKOV3 and to investigate the possible mechanism of the inhibition.Methods The effect of endostatin on SKOV3 cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electronic microscopy.BCL-2 and BAX expressions were measured in SKOV3 cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot.Results Endostatin inhibited SKOV3 cell proliferation(P
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Background and purpose:Ovarian cancer is one of three common malignant tumors in the female reproductive system,whose mortality is the highest in all of the gynecological cancers.The genetic instability and hypermutation rate of tumor cells were found to be associated with chemotherapeutic resistance,and accounted for the high recurrence rate and the failure of current treatment.Invasion and metastasis of tumor are closely related to angiogenesis.Experiments in vivo and in vitro have confirmed that there are many kinds of promoter and suppressor of angiogenesis in primary malignant tumor cells.Angiogenesis could be a potential molecular target for cancer treatment.Endostatin is a specific inhibitor of vessel endothelium.Animal experiments have confirmed that Endostatin could inhibit tumor growth through anti-angiogenesis and result in the decrease of invasion and metastasis of cancer,and it would not easily induced drug resistance in the cells.Thus Endostatin could be safely used for a variety of cancer treatments,being able treat any patho-angiogensis.Recently it has been reported that Endostatin may significantly enhance anti-tumor effects when it is combined with traditional treatment methods such as surgery,chemotherapy,radiotherapy and immunotherapy.Although the study of Endostatin was done in vitro,the primary results were very encouraging and provide the rationale for future clinical trials.In this study,we aim to evaluate the inhibitory effect of Endostatin on SKOV_(3) cell and to investigate the possible mechanism of the inhibition.Methods:1.The effects of Endostatin on SKOV_(3) cells proliferation was studied by means!of MTT.2.The cell apoptosis and cell cycle were detected by transmission electron microscope and cell flow cytometry.3.bcl-2 and bax expression were determined in SKOV_(3) cell by immunocytochemistry,RT-PCR and Western blot analysis.Results:Endostatin inhibited SKOV_(3) cell proliferation,and the value of A_(490) of 15?g/ml 72 h Endostation group(0.454) was much lower than that of PBS control group(1.369)(P