Anaysis of 268 Cases of Phlegm-dampness Syndrome of PCOS with Hyperandrogenism / 医学研究杂志

Objective Through the collection and analysis of clinical features and biochemical indices data got from 436 women who were diagnosed as phlegm-dampness syndrome of PCOS,in order to provide the reference for the personalized treatment for disease and prevention of long-term complication.Methods We collected 436 cases of patients diagnosed with phlegm-dampness syndrome of PCOS and 62 cases of control group from December 2014 to August 2017.We analysied and compared of three groups of clinical and biochemical indicators.Results The incidence of hirsutism,acanthosis nigricans and excessive fat of phlegm-dampness syndrome of PCOS + HA subgroup were higher than phlegm-dampness syndrome of PCOS + non-HA subgroup(P ＜ 0.05).The LH and LH/FSH of of phlegm-dampness syndrome of PCOS + HA subgroup were higher.The level of FSH and SHBG was lower than phlegm-dampness syndrome of PCOS + non-HA subgroup and the control group(P ＜ 0.05).The TC,TG and LDL of phlegm-dampness syndrome of PCOS + HA subgroup were higher than phlegm-dampness syndrome of PCOS + non-HA subgroup and the control group(P ＜ 0.05).The FPG,FINS and HOMA-IR of phlegm-dampness syndrome of PCOS + HA subgroup is higher than phlegm-dampness syndrome of PCOS +non-HA subgroup and the control group(P ＜ 0.05).Conclusion The patients with phlegm-dampness syndrome of PCOS + HA have higher incidence of hirsutism,acanthosis nigricans and excessive fat.The patients with phlegm-dampness syndrome of PCOS + HA have serious sex hormone metabolic disorders.The patients with phlegm-dampness syndrome of PCOS + HA have serious glucolipid metabolic disorders.

Distribution of TCM syndromes in patients with polycystic ovary syndrome and clinical characteristics of syndrome types / 实用医学杂志

Objective To analyze the distributions of Chinese medical syndrome types of polycystic ovary syndrome(PCOS),and explore the differences of clinical features between different syndromes. Methods The data of 496 PCOS patients and the data of reproductive endocrine and metabolic indexes were collected and ana-lyzed retrospectively. Results The results of retrospective analysis:of the 469 patients with PCOS,163 cases were phlegm and blood stasis syndrome(34.75%),157 phlegm dampness syndrome(33.48%),90 kidney liver depression syndrome(19.19%),and 59 kidney deficiency and blood stasis syndrome(12.58%). Compared with the patients with PCOS of kidney liver depression syndrome and kidney deficiency and blood stasis syndrome,the score of acanthosis nigricans as well as the levels of BMI,WC,HC,SHBG,FAI,FPG,FINS,HOMA-IR,TG, CHOL,LDL,ApoB,and ApoB/ApoA-I ratio were significantly increased(P < 0.05),but the level of HDL and ApoA-I significantly decreased in the patients of phlegm and blood stasis syndrome and phlegm dampness syndrome (P<0.05).WHR was higher in the patients of phlegm and blood stasis syndrome than in the patients of kidney liver depression syndrome and kidney deficiency and blood stasis syndrome(P<0.05).Compared with the patients with phlegm and blood stasis syndrome,the level of ApoB and the ApoB/ApoA-I ratio were lower in the patients of phlegm dampness syndrome(P<0.05),the level of FSH and LH were higher in the patients of kidney deficiency and blood stasis syndrome(P<0.05).When compared with the patients of phlegm dampness syndrome,the level of LH was higher in the patients of kidney deficiency and blood stasis syndrome(P<0.05),but the level of T was lower in the patients of kidney liver depression syndrome(P < 0.05). Conclusions Compered with the PCOS patients with phlegm dampness syndrome,kidney liver depression syndrome,kidney deficiency and blood stasis syndrome, phlegm and blood stasis syndrome patients show more serious reproductive endocrine and metabolic disorder.

Clinical Features of Type 2 Diabetes Mellitus Patients with Polycystic Ovary Syndrome / 医学研究杂志

Objective To provide an evidence to type 2 diabetes with PCOS patients,the study compared the clinical features between T2DM,AGT,IR and Non-IR patients.Methods A analysis on 30 T2DM,69 impaired glucose tolerance,87 IR and 31 Non-IR patients of PCOS was performed.Results WHR of the former three groups was significantly higher than the non-IR group(P ＜ 0.05).The systolic pressure and progress of T2DM patients were significantly higher than IR and Non-IR groups(P ＜ 0.05).In T2DM,AGT and IR groups,SHBG level was significantly lower than Non-IR group.In PCOS + T2DM group,FPG,2h blood glucose,HOMA-IR and LDL were significantly higher than the three latter groups(P ＜ 0.05).In PCOS + T2DM group,LN (HOMA-β) was significantly lower than AGT and IR groups,2h blood insulin was significantly lower than AGT group (P ＜ 0.05).PCOS A GT group,FPG,2h blood glucose,2h blood insulin were significantly higher than IR and non-IR.In PCOS + T2DM and AGT groups,TG,TC/HDL,TG/HDL,LDL/HDL,APOB/APOA was significantly higher than IR group and non-IR group (P ＜ 0.05),whlile TG/HDL,LDL/HDL,APOB/APOA of IR group was significantly higher than non-IR group(P ＜ 0.05).Conclusion T2DM patients with PCOS have long progress,with β-cell sensitivity decreased and had higher lipid level than AGT,IR patients.

Clinical features of patients with polycystic ovary syndrome with different SHBG levels / 实用医学杂志

Objective To investigate the role of SHBG in PCOS. Methods Three hundred and ten PCOS patients were divided into the low-SHBG group , the normal-SHBG group , and 95 healthy women were enrolled in the healthy control group. Results (1) In the low SHBG group, the incidences of IR and MS were higher than those in the the normal SHBG group and the healthy control group (P < 0.05); (2) In the low SHBG group, DHEAS was significantly higher than that in the normal SHBG group (P < 0.05); (3) In the low SHBG group, FINS, HOMA-IR, TG, TC/HDL, TG/HDL, LDL/HDL were significantly higher than those in the normal SHBG group and the healthy control group , but HDL was significantly lower than that in the normal SHBG group (P < 0.05); (4) SHBG was positively correlated with HDL, but was negatively correlated with FPG, FINS, HOMA-IR, TG, TC/HDL, TG/HDL, LDL/HDL. Conclusions Uner the low SHBG level, PCOS patients have high levels of DHEAS, and SHBG may be a risk factor for MS, IR and dislipidemia.

Discussion on Protocol Design and Implementation of Effect on Live Birth Rate of Polycystic Ovary Syndrome Treatment with Acupuncture Combined with Clomiphene Citrate / 世界科学技术-中医药现代化

Protocol design and implementation are important steps for clinical trial . This article discussed key issues on effect of live birth rate by polycystic ovary syndrome ( PCOS ) treatment with acupuncture combined with clomiphene citrate in the randomized controlled trial ( RCT ) process . For example , the trial designs ( i . e . , trial design characteristics , selection of featured therapeutic effect index ) , clinical trial implementation ( i . e . , or-ganization construction , quality control , and etc . ) . This summarization provided references for the design and implementation of clinical trial plan .

Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome / 中西医结合学报

Traditional Chinese medicine (TCM) usually views polycystic ovary syndrome (PCOS) as a menstrual disease or infertility disease. Reproductive dysfunction in PCOS is characterized by ovarian androgen excess and disturbance of follicular development, and its main clinical manifestations include delayed menstruation, scant menstruation, amenorrhea or infertility. Insulin resistance is a key pathological mechanism of PCOS. "Tiangui" (kidney essence) as a sex-stimulating essence in female in TCM theory, is essential to the menstruation and pregnancy of women. The disturbance of Tiangui (including time, status and rhythm) would result in female reproductive problems. Current studies of Tiangui indicate that ovary is the target organ of PCOS treatment, and its functional characteristics are consistent with the properties of Tiangui in time frame, state form and rhythm cycle. It is then concluded that ovarian dysfunction in PCOS can be expressed as disorder of Tiangui.

Influence of leptin signal transducting system on ovary function in the db/db mouse / 中华内分泌代谢杂志

Four genotypic mice were constructed, by ovary transplantation, with body leptin receptor (LR) + and ovary LR - in Group A, body LR + and ovary LR + in Group B, body LR - and ovary LR + in Group C, as well as body LR - and ovary LR - in Group D respectively. Mice in C and D groups showed significant differences in glucose and fat metabolism as well as ovarian function as compared with A and B groups. Leptin signal transducting system exists in the ovary. Ovarian failure was induced by hyperlipidemia and lowered gonadotropin levels, but not by defective leptin signal within ovaries in the db/db mice.

Effects of ginseng total saponins on nerve growth factor expression in rat with estradiol valerate-induced polycystic ovaries / 中西医结合学报

OBJECTIVE: To investigate the effect of ginseng total saponins (GTS) on expression of nerve growth factor in rat with polycystic ovaries. METHODS: A total of thirty rats were randomly divided into normal control group, untreated group and GTS group. Polycystic ovaries were induced by a single intramuscular injection of 4 mg estradiol valerate (EV) in rats of the untreated group and GTS group. The rats in the GTS group were administered 50 mg/kg GTS every other day by intraperitoneal injection for 30 days after the EV injection. The expressions of NGF in the ovaries, pituitary and hippocampus were observed by method of immunohistochemistry. RESULTS: Comparison with the normal control group revealed that the expressions of NGF in ovaries, pituitary and hippocampus of rats in the untreated group were increased (P<0.05). The ovarian morphology of the GTS group was almost as normal as that of the normal control group. As compared with the untreated group, the expression of NGF in ovaries of the rats in GTS group was obviously decreased (P<0.05), while the expression of NGF in pituitary and hippocampus showed on difference. CONCLUSION: GTS can decrease the expression of NGF in ovary tissue in rats with polycystic ovaries induced by EV, which may be its mechanism in lessening the polycystic ovary.

Induction of insulin resistance induced by PI-3K inhibitor in porcine granulosa cells / 中华妇产科杂志

Objective To investigate the biological effects of insulin resistance(IR)on the porcine granulosa cells which iS induced by wortmannin,the PI-3K inhibitor and mediated by key molecules including GLUT4 and MAPK during insulin signaling.Methods The model of IR porcine granulosa cell was established in in vitro culture by treatment of wortmannin,and was assessed the amount of3H glucose uptake as well as medium glucose levels by glucose oxidase method.The protein and mRNA expression of GLUT4 and MAPK were evaluated by immunofluorescence and RT-PCR respectively.Resuits The glucose intake was decreased by 40% with treatment of wortmannin at 1.5 μmol/L(P＜0.05).GLUT4 and MAPK were localized mainly to cytoplasm of grantdose cells.When granulosa cells were insulin resistant,the expression of GLUT4 was down-regulated whereas MAPK was up-regulated as compared with the controls.Conclusions Wortmannin treatment can lcad to decreased expression of GLUT4 and increase of IR granulose cells.This metabolic phenotype could induce increased expression of MAPK and mitogenic potential,indicating the cross-talk between two pathways of insulin signaling within ovarian cells.

Multicentre Investigation of the Traditional Chinese Medicine Named YanTing Medincinal Broth on Retention Enema Treatment of Chronic Pelvic Inflammatory Disease / 医学研究杂志

Objective To evaluate the clinical effectiveness and the safety of the traditional chinese medicine named YanTing Medincinal Broth on retention enema treatment of chronic pelvic inflammatory disease.Methods The research method of random,multicentre and parallel contrast were used.There are 92 cases divided into retention enema group and suppository contrast group at random,there are 47 cases in retention enema group and 45 cases in contrast group.Respectively use retention enema method with YanTing Medincinal Broth and the other method with KangFu inflammation eliminated suppository to treat the chronic pelvic inflammatory disease.The course of the treatment are all 10 days.Traditional chinese medicine syndrome and clinical physical signs are observed before and after the treatment in every group,the contrast curative effect are observed at the same time.Results Traditional chinese medicine syndrome and clinical physical signs are all improved than before treatment(P

Therapeutic effects of Jinqi Jiangtang tablet on women with polycystic ovary syndrome / 中西医结合学报

OBJECTIVE: To observe the therapeutic effects of Jinqi Jiangtang Tablet (JQJTT), a commonly prescribed recipe for type 2 diabetes in traditional Chinese medicine, on obese women with polycystic ovary syndrome (PCOS). METHODS: Twenty-four obese women with PCOS were prescribed JQJTT for 3 months and the changes of carbohydrate metabolism and biochemical indicators, including menstrual cycle, body mass index (BMI), the level of blood sex hormone, area under curve (AUC) of glucose and insulin during oral glucose tolerance test (OGTT), and testosterone responses during human chorionic gonadotrophin stimulation ovary test were observed. RESULTS: After treatment, there were significantly reductions in BMI (P<0.05), serum glucose level at 30 min during OGTT (P<0.05) and testosterone levels at eighteen hours during human chorionic gonadotrophin stimulation ovary test (P<0.05). In addition, nine patients had improved cycles among sixteen completed patients, and one patient with clomifene resistance got pregnant with response to clomifene treatment. CONCLUSION: The beneficial effects of JQJTT may result from improvement of carbohydrate metabolism and reduction in androgen biosynthesis in women with PCOS.

Regulation of Tiangui and viscera function / 中华中医药杂志

Tiangui,a specific terms in TCM,is a special material that can regulate human reproduction and development. It stems from congenital kidney essence and is nourished by acquired nutrients with the nature of time-limit,rhythmicity and status. Tiangui promotes abundant CHONG meridian with unobstructed REN meridian,participates in the production and regulation of menstruation,and prepares for conception of women. According to the holism of TCM,person is a unified whole,Tiangui's generation and functional role must be completed under the condition of normal five viscera' function,especially the sufficient kidney essence,although the 'to'and 'exhaustion'of Tiangui is directly affected and regulated by congenital kidney essence. Therefore the synergistic effect of the five viscera can not be ignored on Tiangui,although the congenital kidney essence occupies an important position.

ZHU Dan-xi’s discussion on obese female reproductive dysfunction from phlegm-dampness constitution / 中华中医药杂志

Phlegm is an important etiopathogenisis of female reproductive dysfunction in TCM.ZHU Dan-xi put forward the famous constitution theory of "phlegm in fat people",and there was internal regularity in pathogenesis of wemen with phlegm-dampness constitution.His viewpoint guided the clinical treatment objectively and provided a solid theorectical basis of preventing and treating female reproductive dysfunction induced by phlegm-dapness.Therefore,this paper will discuss the relationship between phlegm-dampness constitution and female reproductive dysfunction from the characteristics of pglegm-dampness constitute,correlation of obesity and pglegm-dampness constitute,and so on.

Regulation of berberine and dioscin in expression of glucose metabolism molecular in trophoblast cells / 中华中医药杂志

Objective:To discuss the regulation of berberine and dioscin extracted from traditional Chinese medicine in the expression of protein moleculer related with glucose metabolism in trophoblast cells,such as IRS-1,P1-3K,Glut1,PPAR ?. Methods:To culture the chorion trophoblast cell in the early pregnancy,to induce cells to suffer glucose metabolism obstacle with the use of WortmaninnTake advantage of berberine and dioscin extracted from the Chinese traditional medicine with intervention,simultaneously set the troglitazone(T) and dimthyl(R) for the control group. Detect the gene expression with the use of RT-PCT,simutaniously detect the protein expression in molecular level. Examine the expression in the protein level with the technique of Western blot in combination with the technique of LSM for the expression location of protein moleculer related.Results:① With the induction of WT,the glucose metabolism inside the tropholast cells becomes abnormal,compared with normal(P