The relationship between coagulation, inflammatory factors, cellular immune function and severity of septic shock / 中国基层医药

Objective:To investigate the relationship between the coagulation function, inflammatory factors, cellular immune function and the severity of septic shock.Methods:From February 2018 to February 2019, 97 patients with septic shock admitted to Zhejiang Rongjun Hospital were divided into survival group(62 cases) and death group(35 cases) according to the prognosis of patients at 28 days after admission.Another 50 healthy physical examinees in Zhejiang Rongjun Hospital from February 2018 to February 2019 were selected as the control group.The changes of coagulation function were measured by automatic hemagglutination analyzer.C-reactive protein(CRP) and procalcitonin(PCT) were detected by electrochemiluminescence, and T-lymphocyte subsets were determined by flow cytometry.Results:The fibrinogen (Fib) and D-dimer (D-D) levels in the survival group [(3.54±0.25)g/L, (3.42±1.08)mg/L] and death group [(4.08±0.34)g/L and (5.31±1.45)mg/L] were higher than those in the control group [(3.17±0.38)g/L and (0.53±0.16)mg/L]( F=6.893, 13.245, all P<0.05). The levels of Fib and D-D in the survival group were lower than those in the death group ( P<0.05). The serum levels of CRP and PCT in the survival group [(48.85±6.57)g/L, (4.35±1.28)mg/L] and death group [(132.52±19.48)mg/L, (10.48±1.96)g/L] were higher than those in the control group [(2.76±0.63)mg/L, (0.10±0.03)g/L]( F=48.973, 13.241, all P<0.05). The levels of CRP and PCT in the survival group were lower than those in the death group(all P<0.05). The CD 3+ , CD 4+ and CD 4+ /CD 8+ in the survival group [(62.35±3.87)%, (37.48±2.86)%, (1.40±0.14)] and death group [(54.61±3.46)%, (33.09±2.15)%, (1.07±0.20)] were lower than those in the control group [(75.34±4.35)%, (43.87±1.90)% and (1.89±0.16)] ( F=6.893, 13.245, all P<0.05). The CD 3+ , CD 4+ and CD 4+ /CD 8+ in the survival group were higher than those in the death group( P<0.05). The APACHE Ⅱ score[(9.28±1.35)points] of the survival group was lower than that of the death group[(15.64±2.48)points]( t=16.383, P<0.05). Conclusion:The patients with septic shock have abnormal coagulation function, obvious inflammatory reaction and decreased immune function, and the coagulation function, inflammatory factors and cellular immune function are significantly related to the severity of the disease.

Expression and clinical significance of androgen receptor in human epidermal growth factor receptor-2 enrich breast cancer / 实用医学杂志

Objective To investigate the expression of AR and its relationship with clinicopathological features in human epidermal growth factor receptor-2(Her-2) enrich breast cancer. Mehtods The expression of AR was detected by immunohistochemical staining in 102 patients with Her-2 enrich breast cancer. The relationship between AR expression and its clinicopathological characteristics was analyzed. Results The positive rate of AR expression was 75.5%. Patients in the positive group had a lower level of lymph nodes and Ki-67 value (P 0.05). Conclusion AR was highly expressed in Her-2 enrich breast cancer, which may be a potential target for treatment of Her-2 enrich breast cancer.

Analysis of sites of Toxoplasma gondii SAG2 gene in blood of HIV-positive people in Lincang City,Yunnan Province / 中国血吸虫病防治杂志

Objective To preliminarily understand the genotype characteristics of Toxoplasma gondii in blood of HIV?posi?tive persons in Lincang City,Yunnan Province. Method Two segments of SAG2 gene of T. gondii from blood samples of HIV?positive persons in Lincang City were extracted and amplified by using the nested PCR method and the genotype was identified and compared with the standard strain(Type I)of Toxoplasma gondii. Results Thirty?five SAG2 genes(241 bp)and 35 SAG2 genes(221 bp)of T. gondii were amplified from 170 blood samples of the HIV?positive people,and 4 of each case were selected and digested with enzyme,then 2 aim gene fragments of each case were chosen and compared with the standard strain (Type I)of T. gondii. The digestion of SAG2 gene(241 bp)showed the genotype of the blood samples was Type I or Type II, and the digestion of SAG2 gene(221 bp)confirmed that the genotype was Type I. Conclusion It is preliminarily confirmed that the genotype of T. gondii in blood of HIV?positive persons in Lincang City,Yunnan Province is Type I.

Study and application of surface antigen in tachyzoites of Toxoplasma gondii / 中国血吸虫病防治杂志

Toxoplasma gondii is an intracellular protozoan parasite that infects all warm?blooded animals. The surface anti?gens of T. gondii with the potential for application as antigens of diagnosis and vaccines have been studied extensively in recent years especially for P43 P35 P30 P23 and P22. The studies on the surface antigen in tachyzoites of T. gondii are reviewed in this paper.

Comparation of Toxoplasma gondii separated from HIV-positive people and RH strain GRA6 gene / 中国血吸虫病防治杂志

Objective To comparatively analyze Toxoplasma gondii separated from HIV-positive people and RH strain GRA6 gene. Methods By using the nested PCR the amplification of Dali HIV-positive blood samples and RH strains of Toxo-plasma GRA6 genome was performed. The GRA6 gene amplification positive product was selected and the electrophoresis imag-ing was performed by being digested with the Mse I endonuclease and the gene sequences were measured and analyzed. Re-sults The GRA6 gene fragment 800 bp was successfully amplified and about 600 bp and 200 bp bands were got by Mse I. The sequencing results showed that T. gondii GRA6 gene positive samples had 2 nucleotide variation compared with T. gondii strain RH namely 447 base pair at C becoming G and 623 base pair at G becoming T. At 146 bp and 690 bp the Mse I restric-tion sites TTAA were found. Conclusion The preliminary judgment shows that the Dali HIV-positive T. gondii genotype is consistent with RH strain belonging to genotype I.

Protective immunity of Trichinella spiralis encapsulated larva antigens / 中国血吸虫病防治杂志

Objective To compare the immune protective effects of three antigens of Trichinella spiralis encapsulated larvae on mice.Methods The mice were immunized with Trichinella spiralis encapsulated larvae somatic antigen,encapsulated larva excretory-secretory antigen and encapsulated larva surface antigen,3 times with a 7-day interval,and the adjuvant control and normal control group were set up.Seven days after the final immunization,each mouse was orally challenged with 200 Trichinella spirais larvae.The intestinal adult worms and muscle larvae of Trichinella spiralis of each group were recoveried and examined on Day 7 and Day 30 post-challenge,respectively.The level of 8eruln IgG to antigens of Trichinella muscle muscle larvae wa8 detected by ELISA.Results The intestinal adult worms were reduced by 84.89%.89.73%,85.65%.2.57% in the encapsulated larva somatic,excretory-secretory and surface antigen groups,respectively.The muscle lalwae were reduced by 71.71%,80.98%,73.66%,5.60%, respectively.Adtlltwornl reduction rates(P<0.05) and musclelarva reduction rates(P<0.01) of the encapsulated larva excretory-secretory antigen group and surface antigen group were higher than those of encapsulated larva somatic antigen group.The antibody titers in all the immunized groups increased significantly.and the GMRT values of the encapsulated larva somatic,excretory-secretory and surface antigen groups were 32 798.89,3 474.51,2 984.83,respectively,and were 6.09,7.56,6.50 times higher than those of the normal control group(459.32).Conclusions Trichinella spiralis encapsulated larva antigens can induce strong resistance of host to a subsequent challenge infection.Among these antigens,excretory-secretory antigen is more immunogenic.

OBSERVATION OF THE KINETIC CHANGE ON IgG,T-CELL SUBSETS,IL-2 LEVEL OF MICE INFECTED TRICHINELLA SPIRALIS / 中国人兽共患病学报

AimTo explore the kinetic change of the IgG,T-cell subsets and IL-2 level from the mice infected with T. Spiralis. Methods The level of specific IgG A band IL-2 was determined by ELISA,the percentage of CD4+and CD8+ T-cells were examined by flow cytometry on 7, 14,21,28,35 days after mice infected with T. spiralis respectively. ResultAfter infected with T. spiralis,the level of IgG in mice rised gradually,and reached its peak on the 35th day. The change of T-cell subsets showed that CD4+T cells decreased,which CD8+Tcells increased. The ration of CD4+/CD8+cells decreased,and which was the obvious on the 14thday. It did not recover to normal level even on the 35th day. The IL-2 of level reached the peak on the 7th day after infected,then IL-2 level decreased quickly and lower than that of normal mice on 35th day after infected. Conclusion When the acute phase of T. spiralis infection,the immune function of host was inhibited. The protective immunity of against T. spiralis infection was cellular immunity mainly ,in cooperation with humoral immunity.

Immune Responses to Challenge Infection in Mice Immunized with Trichinella spiralis Adult Worm Soluble Antigen / 中国寄生虫学与寄生虫病杂志

Objective To study the immune response induced by the mice immunized with Trichinella spiralis(T.spiralis) adult worm soluble antigen(AWSAg). Methods T.spiralis AWSAg was prepared to immunize Kunming strain mice. Dynamic changes of IgG, IL 2 and T lymphocyte subsets from immunized mice were determined after challenge infection on d 7, d 14 , d 21 , d 28 and d 35 . Results On d 7 after challenge infection, IgG and CD4 + T cells of immunized group were markedly elevated and persisted higher over the observation period. In contrast, CD4 + T cells and CD4/CD8 ratio were significantly decreased in unimmunized group resulted from immune suppression after infection. IL 2 levels reached the peak on d 7 and persisted in high level from d 7-d 21 in both immunized and unimmunized group after infection, then decreased gradually. Till 35 days after infection, IL 2 level was still higher than the normal mice. Conclusion Mice immunized with AWSAg of T. spiralis produced a potential cellular and humoral immune response.