RESUMO
In this study, we assessed the effects of shear stress on the expression of plasminogen activator(tPA and uPA) mRNA in cultured NRK-52E cells (a kidney proximal tubular epithelial cell line of normal rat origin) and investigated the mechanism of tubulointerstitial extracellular matrix (ECM) remodeling in the early stage of diabetic nephropathy (DN). The cultured NRK-52E cells were exposed to shear stress of 5 and 10 dyn/cm2 for 1, 3 and 6 hours respectively. Semi-quantity RT-PCR was used to detect the expression of tPA and uPA mRNA. Shear stress down-regulated the expression of tPA and uPA mRNA in cultured NRK-52E cells in a magnitude and time-dependent way. The results suggested that the increased tubular shear stress in the early-stage of DN could decrease the expression of tPA and uPA in renal proximal tubular cells, lead to the reduction of tubulointerstitial fibrinolytic activity and involve in the remodeling of ECM.
Assuntos
Animais , Humanos , Ratos , Linhagem Celular , Nefropatias Diabéticas , Patologia , Células Epiteliais , Biologia Celular , Metabolismo , Patologia , Matriz Extracelular , Metabolismo , Túbulos Renais Proximais , Biologia Celular , Metabolismo , Patologia , RNA Mensageiro , Genética , Metabolismo , Resistência ao Cisalhamento , Ativador de Plasminogênio Tecidual , Genética , Metabolismo , Ativador de Plasminogênio Tipo Uroquinase , Genética , MetabolismoRESUMO
Objective To evaluate the treatment effect of using the double frequency Laser 532 to treat the pathological changes of retina due to diabetes before proliferation. Methods To condense the rays of the wavelength 532nm green laser on the pathologically changed retina and the case-pattern rays on the yellow spot,the full ray-condensed retina should be dealt with after the ray condensation of the yellow spot. During the following 8 months, close attention should be paid to the improvement of the pathologically changed retina, the fadeaway of the dropsy of the yellow spot and the change of eyesight after the operation. Results 67 of 86 persons were improved in their eyesight, with the percentage of 78. 5% ,17 persons(19.5% ) didn't improve and only 2 (2 %) were worse. Conclusion The double frequency laser 532 is effective in treating the pathologically changed retina due to diabetes before proliferation, and has obvious effect in improving the eyesight of the persons with the limited yellow-spot dropsy through the ray condensation.