Effects of varying dilutions, pH, temperature and cations on spermatozoa motility in fish Larimichthys polyactis.

The objectives of this present study were to assess the effects of varying dilutions, pH, temperature and cations on spermatozoa motile parameters (SMPs) in fish Larimichthys polyactis. Optimal SMPs were observed when semen was diluted in artificial seawater (ASW) at a ratio of 1 to 100, with temperature of 10oC and pH 8.0. The spermatozoa of L. polyactis were immotile in distilled water and motile in solution containing different cations. Maximum SMPs were obtained in each solution containing 0.4 mol NaCl, 0.4 mol KCl, 0.2 mol CaCl2 and 0.2 mol MgCl2. This study provides baseline knowledge of L. polyactis spermatozoa sensitivity of pH, temperature and cationic effects.