Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field.

We studied biophoton characteristics of Madin-Darby canine kidney (MDCK) cells under the influence of H2O2 by employing a photomultiplier tube (PMT) and a fluorescence microscope. H2O2 was used for producing reactive oxygen species (ROS) in the measurement. Images from a fluorescence microscope show an increase of photon intensity emitted from the sample due to H2O2. By using a PMT we measured quantitative change in biophoton emission with application of H2O2 to the MDCK cell culture, found that the increase of the biophoton is dependent upon the amount of H2O2. The agreement between the results of the PMT and the fluorescence microscope suggests the possibility of quantitative measurement of the influence of ROS on living tissue or cell. In addition we applied a 60 HzAC magnetic field on the cells to investigate the change in reaction between MDCK cell and ROS. It showed that a decay of chemiluminescence intensity has taken a different path following exposure to the magnetic field. As a result, the PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS.

Relative potency of lambdacyhalothrin and cypermethrin applied as thermal fogs for the control of houseflies (Musca domestica) and mosquitos (Aedes aegypti).

Two highly active synthetic pyrethroid insecticides, lambdacyhalothrin and cypermethrin, were evaluated as thermal fogs against houseflies (Musca domestica Linnaeus) and mosquitos (Aedes aegypti Linnaeus). Lambdacyhalothrin (OMS 3021) showed an average of 2.5 times more knockdown activity and over 5 times more adulticidal activity than cypermethrin against Musca domestica and Aedes aegypti. These results demonstrate that lambdacyhalothrin is highly effective at very low rates as a thermal fog against Ae. aegypti and M. domestica. Commercially available formulations of 2.5% and 5% lambdacyhalothrin can be diluted either with water for ULV cold aerosol space-spraying or with diesel/kerosene for thermal fogging at recommended application rates of 0.5-1 g ai/ha for mosquito control and 2 g ai/ha for housefly control. Due to the very low rates of application, formulated products of lambdacyhalothrin are unlikely to present any acute hazards in normal use. The low dosages required to bring about rapid control of houseflies and mosquitos make this new pyrethroid insecticide particularly cost-effective. Coupled with its good residual activity (Jutsum et al, 1984), lambdacyhalothrin can be adopted as a powerful tool in integrated pest management program for the control of medically important pests and vectors.

Cis-platinum-adriamycin-cyclophosphamide in the treatment of advanced-recurrent cervical cancer.

Twenty-two patients with advanced or recurrent cancer of the cervix treated with cis-platinum, adriamycin and cyclophosphamide were evaluated. Nine of 22 (41% achieved objective response (2 CR + 7PR), 9/22 (41%) stable disease, 4/22 (18%) progressive disease. The mean duration of response was 24.6 weeks, with a range of 18-28 weeks. Patients with poorly differentiated histologic grade and without previous treatment had better response rates. Responders had longer survival (mean 63.5 + weeks) than nonresponders (mean 30.5 weeks). Commonly encountered toxicities were alopecia, nausea and vomiting, and leucopenia, which were all tolerable.

Comparacion de la biodisponibilidad de una formula micronizada y otra ultramicronizada de griseofulvina en el hombre. / Comparison of the bioavailability of a micronized and other ultramicronized formula in the man

Se comparo la biodisponibilidad de 500 mg de una formula micronizada de griseofulvina, con dos nuevas formulas ultramicronizadas del agente, dos tabletas de 165 mg y una tableta de 330 mg, en 16 voluntarios varones, sanos, segun un protocolo de estudio aleatorio y cruzado. En base a las concentraciones plasmaticas de griseofulvina, medidas a intervalos especificados durante um periodo de 48 horas, se determinaron los principales parametros de biodisponibilidad (area bajo la curva de concentracion plasmatica en funcion del tiempo, concentracion plasmatica maxima y tiempo necesario para alcanzar la concentracion plasmatica maxima), y los mismos se evaluaron estadisticamente. Los resultados demostraron que una tableta ultramicronizada de 330 mg era bioequivalente a dos tabletas ultramicronizadas de 165 mg de griseofulvina y que ambas posologias de griseofulvina ultramicronizada eran bioequivalentes a 500 mg de la formula micronizada de griseofulvina