Inhibitory effect of octreotide on prolieration of rat hepatic stellate cells / 吉林大学学报(医学版)

Objective To investigate the effects of octreotide (OCT) on gene and protein expressions of collagen Ⅰ,collagen Ⅲ and matrix matrixmetlloproteinases 2 (MMP-2)in hepatic stellate cells(HSC) in vitro,and to clarify the mechanism of its anti-hepatic fibrosis.Methods A rat hepatic stellate cell line,rHSC-99,was treated with OCT.Cell proliferation was assessed by MTT colorimetric assay.The contents of collagen Ⅰ,Ⅲ were examined by ELISA;RT-PCR was used to detect mRNA expressions of MMP-2,collagen Ⅰ and collagen Ⅲ.Results Compared with control group,OCT significantly inhibited rHSC-99 proliferation in vitro (P