Practical Research on the Teaching of "Medical Interpersonal Communication" Integrate into the Red Doctor’s Spirit for Students Majoring in Public Health Management / 中国医学伦理学

The red doctor’s spirit is an important spiritual guidance for the cultivation of medical talents during the development of medical and health services in China. The teaching reform, which is oriented towards position competence, requires that students majoring in public health management should not only master solid medical knowledge and professional skills, but also possess an ability to handle medical public relations, such as shaping an excellent image of the organization, improving the medical interpersonal communication, and managing public relations crisis. Under the guidance of inheriting the red gene and innovating the medical education, the driving force of learning should be "serving the people to improve their health" led by "political firmness" , the learning goal "striving for perfection" inspired by "excellent technology" , and the working criterion "respecting patients, involving full participation and shouldering public responsibility" embodied in "making selfless dedication and helping the wounded and dying" . The article based on the characteristic culture of red medicine in the Air Force Medical University, applied the teaching method of role-playing case simulations to explore the teaching practice road of "theory-experience-practice-reflection" .

Research progress of ionizable lipid nanoparticles for siRNA delivery / 药学学报

Small interfering RNA (siRNA) is the initiator of RNA interference and inhibits gene expression by targeted degradation of specific messenger RNA. siRNA-mediated gene regulation has high efficiency and specificity and exhibits great significance in the treatment of diseases. However, the naked or unmodified siRNA has poor stability, easy to degrade by nuclease, short half-life, and low intracellular delivery. As an emerging non-viral nucleic acid delivery system, ionizable lipid nanoparticles play an important role in improving the druggability of siRNA. At present, one siRNA drug based on ionizable lipid nanoparticles has been approved for the treatment of rare disease. This review introduces the research progress in ionizable lipid nanoparticles for siRNA delivery, focusing on the effect of each component of lipid nanoparticles on the efficiency of siRNA-mediated gene silencing, which provides new references for the studies on ionizable lipid nanocarriers for siRNA delivery.

The Current Situation and Countermeasures of Ethics Education for Clinical Medical Postgraduates / 中国医学伦理学

In recent years, clinical research has developed rapidly in China and gradually converged with international standards. The importance of ethics education in clinical research for clinical medical postgraduates has become increasingly prominent. From the purpose of promoting the ethics education of clinical medical postgraduates, this paper expounded the significance of carrying out the ethics education of clinical research for clinical medical postgraduates, analyzed the current situation and problems of the ethics education of clinical research for domestic postgraduates, and put forward countermeasures and suggestions for strengthening the ethics education of clinical medical postgraduates. Specifically, this included increasing the degree of attention and rationally allocating teaching resources; paying attention to the transformation of theory into practice, and increasing the relevant curriculum; defining the training objectives, and conducting the staged cross-cultivation; adopting various teaching methods, and improving the training effect; utilizing clinical research practice to develop the ethics ability of clinical research.

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1 / 神经科学通报·英文版

Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures. Recent studies have shown that complement component 3 (C3) aggravate the neuronal injury in epilepsy. And our previous studies revealed that TRPV1 (transient receptor potential vanilloid type 1) is involved in epilepsy. Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood. We found that in a mouse model of status epilepticus (SE), complement C3 derived from astrocytes was increased and aggravated neuronal injury, and that TRPV1-knockout rescued neurons from the injury induced by complement C3. Circular RNAs are abundant in the brain, and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV1 and exacerbated neuronal injury. Mechanistically, disorders of neuron–glia interaction mediated by the C3–TRPV1 signaling pathway may be important for the induction of neuronal injury. This study provides support for the hypothesis that the C3–TRPV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1 / 神经科学通报·英文版

Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures. Recent studies have shown that complement component 3 (C3) aggravate the neuronal injury in epilepsy. And our previous studies revealed that TRPV1 (transient receptor potential vanilloid type 1) is involved in epilepsy. Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood. We found that in a mouse model of status epilepticus (SE), complement C3 derived from astrocytes was increased and aggravated neuronal injury, and that TRPV1-knockout rescued neurons from the injury induced by complement C3. Circular RNAs are abundant in the brain, and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV1 and exacerbated neuronal injury. Mechanistically, disorders of neuron-glia interaction mediated by the C3-TRPV1 signaling pathway may be important for the induction of neuronal injury. This study provides support for the hypothesis that the C3-TRPV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.

Expression and prognostic effect of DEPTOR in rectal carcinoma / 局解手术学杂志

Objective To clarify the expression of DEPTOR in rectal cancer,and to further explore the relationship between the expres-sion level of DEPTOR and histopathology and prognosis,in order to provide reference for the clinical diagnosis and treatment of rectal cancer. Methods The clinical data of 102 patients who underwent radical resection of rectal cancer in our hospital from January 2011 to January 2013 were analyzed retrospectively.The expression of DEPTOR in cancer tissues and adjacent tissues were evaluated by immunohistochemis-try and immunoblotting.The patients were divided into high expression group and low expression group by the median value of integrated opti-cal density(IOD);the relationship between the expression level of DEPTOR and clinical,histopathology and prognosis was analyzed. Results The results ofImmunohistochemistry and immunoblotting showed that the expression level of DEPTOR in cancer tissues was higher than that in adjacent tissues,the differences were significant(P<0.05). Univariate analysis showed that there was no significant differences in gender, age,and BMI(P>0.05),and there were significant differences in tumor diameter,T stage,N stage,and differentiation between the high-ex-pression and low-expression group (P<0.05).The independent influencing factors of DEPTOR expression was analyzed by the Logistic re-gression model,which showed that T stage and tumor diameter were independent influencing factors of high expression of DEPTOR.Compared with low expression group,the serum CEA level in patients with high expression group was higher,the differences was statistically significant ( P < 0.05 ). There was no significant difference in serum CA199 level between the expression group and the low expression group (P>0.05).Spearman correlation analysis showed that the expression level of DEPTOR was positively correlated with serum CEA level in rectal cancer patients (r=0.509,P<0.01).Compared with low expression group,the 5-year cumulative recurrence rate and the 5-year cumulative mortality rate in the high-expression group of DEPTOR were higher,the differences were statistically significant ( P <0.05). Conclusion DEPTOR is highly expressed and is associated with the degree of disease progression in rectal cancer,its elevation suggests a poor prognosis.

Preliminary study on WPBL teaching method in microbiological test teaching / 中华医学教育探索杂志

Objective To explore the effect of WPBL teaching method in microbiologicla test teach-ing. Method A total of 120 students majoring in clinical laboratory medicine were divided into the experi-mental group (WPBL teaching) and the control group (the traditional teaching) with 60 in each group in 2015. Results Questionnaire survey showed that in the experimental group, 92.6％of the students believe that WPBL teaching method can stimulate learning interest and promote student self-directed learning, 97.3％ believe that WPBL teaching method can improve the ability to analyze and solve problems, 94.7％believe that WPBL teaching method can strengthen teamwork and promote learning exchanges, and 99.6％hold that WPBL teaching method can help them recognize the importance of microbiological tests in the di-agnosis of clinical infectious diseases. The final scores and the usual performance of the experimental group were (75.23 ±11.21) and (24.15 ±1.71), respectively, while those of the control group were (63.42 ±8.21) and (20.26±1.48), respectively. The differences were statistically significant (P<0.005). Conclusions PBL teaching method can fully integrate teaching resources, stimulate students' enthusiasm and initiative, improve students' comprehensive qualities, including the ability to analyze and solve problems, teamwork ability and critical thinking ability, and improve the quality and effect of microbiological test teaching.

The Observation of Curative Effect of Budesonide Nebulization Supplying on Post-Infection Cough Undergoing Painless Gastroscopy / 昆明医科大学学报

Objective To observe and compare the incidence of cough among patients with post-infection cough undergoing painless gastroscopy.Methods Seventy-one patients with post-infection cough undergoing painless gastroscopy were enrolled and randomly divided into two groups.The observation group (34 cases) received budesonide nebulization before gastroscopy and the control group (37 cases) was performed gastroscopy directly.We observed the incidence of cough at different time point and the changes of circulationduring the operation. Results The incidence of cough between the observation group and the control group showed statistically significant differences (P＜0.01) . The changes of circulation showed statistical differences between two groups in the process of gastroscopy (P＜0.05).Conclusion Budesonide nebulization supplying can decrease the incidence of post-in-fection cough among patients undergoing painless gastroscopy.

Up-regulation of Keap-1 inhibits the nuclear import of Nrf-2 and decreases cisplatin resistance in ovarian cancer Skov3/DDP cells / 基础医学与临床

Objective To test the status of Keap-1/Nrf-2 pathway in cisplatin-resistant Skov3/DDP cells,and the contribution of these molecular in cisplatin (cis-pt) resistance.Methods Skov3/DDP was divided into four groups:blank,cis-pt,Keap-1 up-regulated and Keap-1 plus cis-pt.Keap-1 was up-regulated in Skov3/DDP cells by trans-faction with pFlag-CMV-Keap-1 plasmid which was validated by expression of flag and increase of Keap-1 level using Western blot.The effect of Keap-1 up-regulation on Nrf-2 re-distribution was analyzed by Western blot.FCM was employed to detect apoptosis.ROS level was measured by DHE staining.Results The expression of Keap-1 was decreased obviously in Skov3/DDP cells compared with Skov3 cells (P＜0.05),Nrf-2 in Skov3/DDP cells detected mainly in nucleus.Then,the Keap-1 was successfully up-regulated (3.5 fold) by exogenous expression of pFlag-CMV-Keap-1 plasmid,as a result,nucleus portion of Nrf-2 was significantly reduced (0.2 fold).Next,the FCM results showed significant increase of apoptosis in Keap-1 up-regulated Skov3/DDP cells after cis-pt administration (P＜0.05).Finally,ROS level was significantly raised in Skov3/DDP cells treated with cis-pt plus Keap-1 up-regulation.Conclusions Up-regulation of Keap-1 reduced the nucleus portion of Nrf-2 and reverses cisplatin resistance in Skov3/DDP cells.

Correlation between the Expression of High Temperature Requirement Serine Protease A1 in Nucleus Pulposus Tissue and the Degree of Intervertebral Disc Degeneration / 中国医学科学院学报

Objective To investigate the correlation between the expression level of high temperature requirement serine protease A1 (HtrA1) in nucleus pulposus and the degree of intervertebral disc degeneration (Pfirrmann grade).Methods Thirty-six patients who underwent excision of nucleus pulposus were examined by magnetic resonance imaging (MRI) before operation,and the Pfirrmann grading of all patients was performed according to the sagittal T2 weighted MRI.The expression of HtrA1 in nucleus pulposus tissue was detected by reverse transcription polymerase chain reaction and Western blot.The correlation between the expression level of HtrA1 in nucleus pulposus tissue and Pfirrmann grade was analyzed.Results MRI in all 36 patients showed that there were 3 cases of Pfirrmann grade Ⅰ,10 cases of grade Ⅱ,11 cases of grade Ⅲ,7 cases of grade Ⅲ,and 5 cases of grade Ⅴ.The mRNA and protein expressions of HtrA1 in nucleus pulposus tissue increased with the increase of Pfirrmann grade.There were significant differences in the expression level of HtrA1 among different Pfirrmann grade groups (P<0.05) except for the difference between grade Ⅲ and Ⅴ (P>0.05).Spearman rank correlation analysis revealed that there was a rank correlation between expression level of HtrA1 and Pfirrmann grade (P<0.000 1).Conclusion The mRNA and protein expressions of HtrA1 in nucleus pulposus tissue increase with the increase of Pfirrmann grade,indicating HtrA1 is correlated with the degree of intervertebral disc degeneration.

Advances in Tissue-engineered Oral Mucosa / 中国医学科学院学报

The large defect of oral and maxillofacial region doesn't only affect the function and aesthetics but also has an adverse impact on patients' psychology. The traditional way to restore the defects are limited by donor site and secondary trauma. In recent years,the oral mucosal tissue engineering has developed rapidly and provides a new solution for craniofacial reconstruction. Tissue-engineered oral mucosa is an ideal substitute of oral mucosa. It can be used in clinical settings and in vitro experiments. This articles review the recent advances in tissue-engineered oral mucosa and its applications.

Design experiments in the teaching of higher vocational microbiology test / 中华医学教育探索杂志

Objectve To study director-leading teaching method,raise the students' main body consciousness and innovation spirit and improve the teaching effect of microbiology test.Method 158 students majored in laboratory medicine of Grade 2013 were divided in two groups.80 from class 1 were in test group,and the rest from class 2 were served as control group.The clinical microbiological specimens were collected and identified;the experimental class was designed with the experimental teaching mode,while the control group used the traditional experimental teaching mode.Through the experimental evaluation and questionnaire survey,the difference between new teaching model and the traditional teaching mode were evaluated.SPSS 21.0 software was used in the collected data for processing.Result The theoretical results of the experimental class were (88.65 ± 7.64) and the practical skills were (89.72 ± 7.69) and the control group were (79.88 ± 10.64) and (80.55 ± 9.06).The scores of the experimental class were significantly higher than those of the control class,and there and statistically significant difference between the scores of two classes.T values were 6.851 and 5.938 and P values were 0.000 and 0.000 respectively.Survey results show that:90％ of the students agree with the new teaching methods.Conclusion The teaching result of the new teaching mode is superior to the traditional teaching mode.

Biological properties of a new injectable porous microspherical chitosan/hydroxyapatite scaffold / 医学研究生学报

[Abstract ] Objective At present, the majority of injectable tissue engineering bones or carrier stents are gel , whose surface area , intensity, and hardness cannot satisfy the requirements of the repair of complex and varied bone and cartilage defects .This paper evaluated the new injectable microspherical porous chitosan/biological properties of the hydroxyapatite ceramic scaffold . Methods Injectable porous chitosan /hydroxyapatite composite microspheres with mass fractions of 30%, 50%, and 70% were prepared respectively . The hydroxyapatite ceramic ball was obtained by sintering with liquid nitrogen freezing ( liquid nitrogen group ) or without liquid nitrogen pro-cessing ( non-liquid nitrogen group ) as a new carrier of bone tissue engineering scaffold material .The microstructure of the scaffold was observed and the porosity measured under the scanning electron microscope .The mechanical properties were determined through biome-chanical experiments.Human umbilical vein endothelial cells (HUVECs) were grown in the porous chitosan/hydroxyapatite ceramic scaf-fold followed by observation of the growth of the cells and validation of the biological fusion of the scaffold . Results No difference was observed with the naked eye in the ceramic scaffold of different mass fractions in the liquid nitrogen and non -liquid nitrogen groups . Scanning electron microscopy exhibited spherical shape , uniform size, and regular morphology of the ceramic scaffolds in both groups .A large number of irregular pores were seen in the surface of the microspherical ceramic scaffolds treated with liquid nitrogen but not in the surface of those not treated .With increased mass percentage of chitosan/hydroxyapatite , the internal pores were reduced and the interior structure compacted.In the liquid nitrogen group, the scaffold of 50%mass fraction had a significantly larger diameter ([0.48 ±0.11] mm), higher compression intensity ([1.75 ±0.14] MPa), and lower porosity ([79 ±2]%) than that of 30%mass fraction ([0.40 ± 0.08] mm, [1.21 ±0.12] MPa, and [87 ±1]%) (all P<0.05).Electron microscope scanning revealed well -grown HUVECs with multiple synapses in the porous tricalcium phosphate scaffold. C onclusion The porous chitosan /hydroxyapatite ceramic scaffold of 50%mass fraction treated with liquid nitrogen , with its strong mechanical intensity and high biological fusibility , can be used as a new carrier of bone tissue engineering scaffolds .

Analyses on the relative factors regarding diabetic nephropathy / 中华流行病学杂志

Objective To analyze the related factors regarding diabetic nephropathy (DN).Methods A total number of 756 diabetic patients from 2009 to 2011 were analyzed retrospectively.Three groups were formed according to the urinary albumin excretion rates (UAER).Patients with UAER＜20 μg/min was grouped to group A,with UAER from 20 to 200 μg/min as group B,and the others with UAER ≥200 μg/min was grouped to group C.General characteristics and laboratory parameters were then compared and related factors of DN analyzed.Results The constituent ratio of nephropathy was 30.2％ (228/756).Patient's age,duration of disease,both diastolic and systolic blood pressure of group A were significantly higher than the non-DN group (A) (P＜0.05).Patient's age,disease duration,both diastolic and systolic blood pressure of group C were (64.08± 11.71)years,(14.67± 7.34)years,(87.43 ± 14.36)mm Hg,(152.45 ± 18.48)mm Hg,respectively,with statistically significant difference (P＜0.05) between group C and group B.FPG,TG,TC,HDL-C,UA,HbAlc,FINS,FCP of group B were (9.27 ± 3.06) mmol/L,(1.98 ± 0.37) mmol/L,(5.01 ± 1.08) mmol/L,(1.05 ± 0.35) mmol/L,(312.78 ± 39.83) mmol/L,(9.33 ± 1.47)％,(11.45 ± 7.83) μU/ml,(509.73 ±132.78) pmol/L respectively,with significant difference (P＜0.05) between group B and group A.FPG,TG,HDL-C,UA,FINS,FCP of group C were (9.29 ± 3.12)mmol/L,(2.02 ± 0.36)mmol/L,(1.04 ± 0.27) mmol/L,(389.72 ± 46.32) mmol/L,(11.09 ± 8.29) μ U/ml,(575.77 ± 143.29) pmol/Lrespectively,with significant difference (P＜0.05) between group C and group A.UA,FINS,FCP were found with significant differences (P＜0.05) between group C and group B.Data from multivariate logistic regression showed that DNs were related with disease duration,BMI,systolic blood pressure,HbAlc,FPG,UA.Conclusion DN was closely related to the duration,age,blood sugar,blood lipids,blood pressure,uric acid levels of the disease.

Research progress in nano-scaffolds for spinal cord tissue engineering / 国际生物医学工程杂志

Spinal cord injury is a difficult medical problem and need to be solved urgently.Application of tissue engineering to repair spinal cord injury has gradually become a hot spot.It is important to prevent the development of scar tissue while inducing cells' regeneration by using scaffold.Nanotechnology has improved the performance of scaffold because of its superiority.Nanoscaffold has obvious advantages compared with the traditional scaffolds.New scaffold materials can be obtained by nanotechnology.Nanoscaffold can also serve as a good drug carrier,and it may have beneficial effects on biological behaviors of seed cells on its surface,such as differentiation,proliferation and migration,which may promote tissue regeneration and functional recovery and get good results in repairment of spinal cord injury.This article summarized the research progress in recent years in nano spinal cord engineering scaffolds in order to provide a reference for research in related fields.

Discussion on development orientation of normalization education of doctor-patient communi-cation skills in higher medical education / 中华医学教育探索杂志

This paper analyzed and discussed on the importance of doctor-patient communica-tion skills and the differences between foreign countries and China. Meanwhile, it pointed out the drawbacks of doctor-patient communication skills training for medical students in China and proposed some countermeasures including finding out influence factors, searching for reference books, provid-ing guide for doctor-patient communication theory and skills, formulating teaching pattern, building evaluation mechanism, and establishing a communication platform of doctor-patient communication skills for medical students.

Ryanodine downregulates the expression of p-eNOS (Thr495) and improves the functions of rapamycin treated endothelial outgrowth cells / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe the effects of ryanodine on rapamycin treated endothelial outgrowth cells (EOCs).</p><p><b>METHODS</b>The mononuclear cells were harvested from umbilical cord blood by Ficoll density gradient centrifugation, then induced into EOCs and expanded in vitro. The endothelial characteristics of EOCs were identified by immunostaining and fluorescent staining. The EOCs were pretreated with or without ryanodine (10 µmol/L) for 1 h, and then treated with or without rapamycin (10 nmol/L) for 24 h. Proliferation was evaluated by CCK8 and migration was measured by Transwell. The protein expression of EOCs was evaluated by immunobloting technique with total eNOS antibody and phospho-eNOS (Thr495) antibody.</p><p><b>RESULTS</b>Compared with control group, the proliferation and migration capacities of EOCs were significantly reduced while the phosphorylation of eNOS (Thr495) protein was significantly upregulated in rapamycin group (P < 0.05), expression of total eNOS was not affected by rapamycin (P > 0.05). Compared with rapamycin group, the proliferation and migration capacities of EOCs were significantly increased and the phosphorylation of eNOS (Thr495) protein was significantly downregulated in ryanodine + rapamycin group (P < 0.05). The proliferation and migration capacities, the phosphorylation of eNOS (Thr495) protein and the expression of total eNOS were not affected by ryanodine alone (P > 0.05).</p><p><b>CONCLUSIONS</b>Rapamycin reduced proliferation and migration capacities while upregulated the phosphorylation of eNOS (Thr495) protein of EOCs and these effects could be partly reversed by cotreatment with ryanodine.</p>

Impact of number of retrieved lymph nodes and lymph node ratio on the prognosis in patients with stage II and III colorectal cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To discuss the impact of number of retrieved lymph nodes and lymph node ratio(LNR) on the prognosis in patients with stage II and III colorectal cancer.</p><p><b>METHODS</b>Clinicopathological data of 507 patients with stage II and III colorectal cancer were analyzed retrospectively. Follow-up was available in all the patients.</p><p><b>RESULTS</b>The total number of retrieved lymph nodes was 5801, of which 1122 had metastasis. There was a positive correlation between metastatic lymph nodes and retrieved lymph nodes(r=0.171, P<0.01). In stage II colorectal cancer there was a significant difference in 5-year survival rate between patients with more than 12 lymph nodes retrieved and those with less than 12 lymph nodes retrieved(P<0.01). LNR also affected the 5-year survival rate of patients with stage II and III colorectal cancer(P<0.05). In patients with similar LNR, the 5-year survival rate differed significantly among different regions of lymph node metastasis(P<0.05). LNR influenced the prognosis independent of the number of lymph nodes retrieved.</p><p><b>CONCLUSIONS</b>The number of retrieved lymph nodes is a prognostic factor for stage II and III colorectal cancer. More than 12 lymph nodes should be retrieved for better staging and prognosis. LNR is also a prognostic factor in stage II and III colorectal cancer. Regions of lymph nodes metastasis should be considered when evaluating the prognosis of patients using LNR.</p>

Traumatic Segmental Defects of Tibia Treated by Internal Bone Transport Using Adjustable Mono-lateral External Fixation / 中国康复理论与实践

@#ObjectiveTo evaluate the clinical and functional outcome of traumatic segmental defects of the tibia treated by internal bone transport using the the adjustable mono-lateral external fixation.Methods12 patients who underwent internal bone transport using the the adjustable mono-lateral external fixation for traumatic segmental defects in the tibia were reviewed, the length of bone defect was 6～10 cm.ResultsMean follow-up was 14 months. All the cases got primary bone union. The length of time in healing was 9～14 months.ConclusionTraumatic segmental defects of the tibia treated by internal bone transport using the adjustable mono-lateral external fixation is a simple, less traumatic, non-bone graft and effective way.

Chromosomal aberrations in chronic lymphocytic leukemia by interphase fluorescence in situ hybridization and their association with clinical features / 中国实验血液学杂志

This study was aimed to investigate the common chromosomal aberrations in chronic lymphocytic leukemia (CLL) and to explore the relationship between these chromosomal aberrations and clinical features of CLL. Sequence-specific DNA probes (D13S25, RB1, p53, ATM) and one centromeric probe CSP12 were applied to detect del(13q14), del(17p13), del(11q22-q23) and trisomy 12 by using interphase fluorescence in situ hybridization (I-FISH). 9 CLL patients with negative conventional cytogenetics or without mitotic figure were enrolled in this study. The threshold was established using 10 controls without hematopoietic malignancies. The results indicated that compared with the established threshold, all of the 9 CLL patients showed cytogenetic abnormalities. The detection using p53 and D13S25 showed positive in 7 cases, positive was observed in 5 cases by using ATM and in 4 cases by using both RB1 and CSP12. There was significant correlation between the ATM and the hemoglobin level of the patients. In addition, the elevated probability of gaining bulky lymphadenopathy was found in ATM positive patients. It is concluded that the I-FISH is a more rapid and sensitive technique for analysis of chromosome aberrations in CLL. A large series study with long-term follow-up is needed to reveal the role of cytogenetic abnormalities in the determination of CLL prognosis.