RESUMO
Alzheimer's disease(AD)represents the main form of dementia;however,valid diagnosis and treatment measures are lacking.The discovery of valuable biomarkers through omics technologies can help solve this problem.For this reason,metabolomic analysis using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)was carried out on plasma,hippocampus,and cortex samples of an AD rat model.Based on the metabolomic data,we report a multi-factor combined biomarker screening strategy to rapidly and accurately identify potential bio-markers.Compared with the usual procedure,our strategy can identify fewer biomarkers with higher diagnostic specificity and sensitivity.In addition to diagnosis,the potential biomarkers identified using our strategy were also beneficial for drug evaluation.Multi-factor combined biomarker screening strategy was used to identify differential metabolites from a rat model of amyloid beta peptide 1-40(Aβ1-40)plus ibotenic acid-induced AD(compared with the controls)for the first time;lysophosphati-dylcholine(LysoPC)and intermediates of sphingolipid metabolism were screened as potential bio-markers.Subsequently,the effects of donepezil and pine nut were successfully reflected by regulating the levels of the abovementioned biomarkers and metabolic profile distribution in partial least squares-discriminant analysis(PLS-DA).This novel biomarker screening strategy can be used to analyze other metabolomic data to simultaneously enable disease diagnosis and drug evaluation.
RESUMO
To investigate the therapeutic effect of minimally invasive interventional treatment of brain hematoma, 128 patients with brain hematoma were randomly divided into two groups, including observation group [64 cases] and control group [64 cases]. The observation group was performed with minimally invasive interventional therapy and drug conservative therapy was used in the control group. Clinical curative effect, treatment of neural function defect score, and quality of life score were recorded. Total effective rate in observation group was significantly higher [p<0.05] than that in control group [90.6% vs 62.5%]. And the mortality in observation group was significantly lower [p<0.05] than that in control group [3.1% vs 9.4%]. National Institute of Health stroke scale [NIHSS] and Quality of Life [QOL] of the two groups after treatment were improved significantly [p<0.05] compared with those with treatment before; and the improved level of concept in observation group was significantly higher [p<0.05] than that of control group. Therefore, minimally invasive interventional brain hematoma surgery can effectively remove the hematoma in the brain, improve the nerve function of patients caused by trauma and improve the quality of life