Serum exosomal miR-335-5p serves as a diagnostic biomarker of triple-negative breast cancer / 中华内分泌外科杂志

Objective:To investigate the expression and diagnostic value of microRNA-335-5p (miR-335-5p) and Human Vascular endothelial growth factor receptor 1 (VEGFR-1, FLT1) in serum exosomal bodies of patients with triple negative breast cancer (TNBC) .Methods:Gene Expression Omnibus database (GEO) analysis was performed and differentially expressed miRNA in breast cancer tissues was screened. From Jan. 2016 to Nov. 2017, the peripheral blood samples of 56 TNBC patients in People’s Hospital Affiliated to Ningbo University were collected, and the exosomes were isolated and identified. FLT1 was selected as the target gene of miR-335-5p by using bioinformatics analysis. Expression levels of miR-335-5p and FLT1 in serum exosome were detected by qRT-PCR. The relationship between miR-335-5p and clinicopathological parameters of TNBC patients was analyzed. The diagnostic value of miR-335-5p was evaluated by receiver operating characteristic (ROC) , and the survival prognosis of miR-335-5p and FLT1 was analyzed by Kaplan Meier survival curve.Results:The expression of miR-335-5p in the serum exosome of TNBC patients was lower than that of the control group, while the expression of FLT1 in the serum exosome was higher than that of the control group ( P<0.05) . The expression of miR-335-5p was related to tissue grade ( χ2=22.02, P<0.000 1) , degree of differentiation ( χ2=20.67, P<0.000 1) and lymph node metastasis ( χ2=4.667, P=0.030 8) in patients with TNBC ( P<0.05) . The area under ROC diagnosed by miR-335-5p was 0.809 8 (95% CI: 0.726 3-0.893 2, P<0.000 1) . Kaplan-Meier survival analysis showed that the overall survival time of patients with low expression of miR-335-5p was significantly shorter than that of patients with high expression of miR-335-5p ( P=0.004 5) . The high expression of its target gene FLT1 was associated with low survival rate ( P=0.048 0) . Conclusion:Serum exosomal miR-335-5p can be used as an important index to predict the prognosis of TNBF and is expected to play a role in diagnosis and treatment of TNBC.

Effects of early immune enteral nutrition combined with glutamine parenteral nutrition on T-lymphocyte subsets in patients with severe pulmonary infection in intensive care unit / 中国医师进修杂志

Objective:To study the effect of early immune enteral nutrition combined with glutamine (Gln) parenteral nutrition on T-lymphocyte subsets in patients with severe pulmonary infection in the intensive care unit (ICU).Methods:A total of 70 ICU patients with severe pulmonary infection who were treated in Yinzhou People′s Hospital from January 2017 to October 2019 were selected, and the patients were divided into enteral nutrition group and combined group according to a completely random method with 35 cases in each group. Both groups of patients were given conventional treatment; patients in the enteral nutrition group was given immunoenhanced enteral nutrition on the basis of conventional treatment, and those in the combined group was given Gln on the basis of enteral nutrition group. The levels of arterial oxygen partial pressure (PaO 2), arterial oxygen saturation (SaO 2), arterial partial pressure of carbon dioxide (PaCO 2), CD 8+, CD 3+, CD 4+/CD 8+, procalcitonin (PCT), hypersensitive C-reactive protein (hs-CRP) and tumor necrosis factor alpha (TNF-α) before and after treatment were detected and compared between two groups. The treatment efficacy was compared between two groups too. Results:After treatment, the levels of SaO 2 and PaO 2 in combined group were higher than those in enteral nutrition group, the level of PaCO 2 in combined group was lower than that in enteral nutrition group, but there were no significant differences ( P>0.05). After treatment, the levels of CD 3+, CD 4+/CD 8+ in combined group were higher than those in enteral nutrition group [(62.37 ± 6.15)% vs. (59.35 ± 5.24)%, 1.50 ± 0.18 vs. 1.12 ± 0.11], the level of CD 8+ in combined group was lower than that in enteral nutrition group [(33.15 ± 6.11)% vs. (37.72 ± 8.57)%], and there were significant differences ( P<0.05). The levels of PCT, hs-CRP and TNF-αin combined group were lower than those in enteral nutrition group [(3.83 ± 0.82) μg/L vs. (6.47 ± 1.34) μg/L, (6.92 ± 1.25) mg/L vs. (12.72 ± 3.83) mg/L, (92.35 ± 13.05) ng/L vs. (125.26 ± 18.35) ng/L], and there were significant differences ( P<0.05). The total effective rate in combined group was higher than that in enteral nutrition group [88.57%(31/35) vs. 65.71%(23/35)], and there was significant difference ( χ2=5.185, P<0.05). Conclusions:Early immune enteral nutrition combined with Gln parenteral nutrition has significant effects on patients with severe pulmonary infection in ICU, and can improve the level of T-lymphocyte subsets, control infection, and reduce inflammatory factor levels.

Study on correlation between plasma NT-proBNP with essential hypertension complicating left ventricular dysfunction / 国际检验医学杂志

Objective To investigate the clinical values of NT-proBNP in left ventricular enlargement(LVE) and left ventricular dysfunction(LVD) of the patients with essential hypertension(EH) to provide a diagnostic basis for their diagnosis .Methods Plas-ma NT-proBNP concentrations in 120 patients with EH and in 29 normal controls were measured ,then the echocardiography exami-nation was performed to determine the left ventricular ejection fraction ( LVEF) ,left ventricular end-diastolic diameter(LVEDD) , left atrium(LA) and left ventricular systolic diameter(LVSDD) .The correlation between plasma NT-proBNP with LVE and LVD was analyzed .The diagnostic accuracy of NT-proBNP was analyzed by using receiver operating characteristic (ROC) curve .Results There were no statistically significant differences in the aspects of age ,sex and serum creatinine between the EH group and con-trol group ,but plasma NT-proBNP level of the former was significantly higher than that of the latter .The NT-proBNP level in the patients with LVE was significantly higher than that in the patients with normal left ventricle (P<0 .05) .The NT-proBNP level in the LVD patients was significantly higher than that in the patients with normal left ventricular function (P<0 .05) .The plasma NT-p roBNP level was positively correlated with LA ,LVEDD and LVSDD(r=0 .518 ,0 .58 ,0 .48 ,P<0 .01) while negatively crrelated with LVEF(r= -0 .61 ,P<0 .01) .The ROC curve showed that when the NT-proBNP was set at 380 pg/mL ,the sensitivity and specificity of NT-proBNP for diagnosing LVE were 80 .6% and 72 .4% ;which for diagnosing LVD were 80 .8% and 77 .4% ,re-spectively .Conclusion NT-proBNP is closely correlated with multiple ultrasonic indicators reflecting the left ventricular function and its level can reliably reflect the left ventricular contraction function ,which can serve as the marker for screening LVE and LVD in the patients with EH .

Analysis of allergens characteristic in 1172 patients with allergic rhinitis in Changzhou area / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To determine the distribution of allergens in patients with allergic rhinitis during from 2008 to 2015 in Changzhou area.@*METHOD@#Allergy Screen method was used to detect the specifical-allergen IgE levels of 1172 patients with allergic rhinitis. Among the patients, the distribution of all allergens was analyzed. The positive rate was compared with age, gender, season and so on.@*RESULT@#The most common allergens in allergic rhinitis patients in Changzhou were dust mite, fungus, house dust, milk, dander of dog, weed mixture, farina and dander of cat. The more higher positive rates of dust mite, fungus, house dust, milk, dander of dog, dander of cat, eggs were found in teenager group than adult group (P < 0.05). There were more higer positive rates of weed mixture, farina, cashew, cockroaches and crab in teenager group than adult group (P < 0.05). There was no significant difference of positive rate between female group and male group. There was significant difference of variation with seasons.@*CONCLUSION@#Dust mite, fungus and house dust were the most common allergens among patiens with allergic rhinitis in Changzhou area. The positive rates of allergens varied with ages and seasons.

Treatment of intertrochanteric fracture with proximal femoral nail of AO/ASIF / 中国综合临床

Objective To investigate the clinical outcomes of intertrochanteric fracture by the fixation using proximal femoral nail (PFN/PFNA).Methods From Jan.2008 to Dec.2010,56 cases with intertrochanteric fracture,including 18 males and 38 females with an average age of 76 years (range,67 -94 ),were treated by fixation with PFN or PFNA.All patients had closed fractures in the shaft of femur.The average time from injury to operation was 3.2 days (ranging from 2.0 days to 10.0 days).All the patients were followed for 1,2,3,6,12 month after surgery,respectively,and then at least once per year.The clinical outcomes were evaluated according to Harris criteria and radiological examinations.Results The mean operating time of all cases was 55 minutes ( ranging from 40 to 80 minutes),and the mean blood loss during surgery was 80 ml (range,50 - 150 ml).All patients was able to walk with walking aid 3 -7 days after surgery.All the patients with fracture healed after 3.5 - 6.0 months ( mean:4 months ).No complications such as infection,lower limb vein infarction,cut-out or breakage of the implants occurred.Four cases had coxa vara shortening deformation,According to Harris criteria,the clinical results were excellent in 43 cases,good in 9,and moderate in 4,with the rate of patients in excellent and good condition was 92.86％ (52/56).Conclusion PFN/PFNA is an effective and minimally invasive method for intertrochanteric fracture,with minimal invasion,high fixation-strength and optimal biomechanics characteristics.This treatment technique produces a high rate of bone re-union,early functional recovery and less complications.

Concentration of airborne pollen in Beijing city with burkard sampler / 临床耳鼻咽喉头颈外科杂志

Objective:To investigate the airborne pollen concentration in Beijing city during autumn and to grade the Artemisia pollen concentration .Method:Burkard volumetric trap was used to sample the airborne pollen in Beijing city from August 1st 2007 to October 10th 2007. Patients only allergic to mugwort pollen were followed up, and their symptom scores and the mugwort pollen concentration were analyzed statistically.Result:①Mugwort and Humulus(including Cannabis Sativa L) were the main airborne pollen during August and September in Beijing city, accounting for 31% and 51%,respectively; ②Mugwort pollen season was from 8th August to 8th October;③The daily peak concentration of Mugwort pollen was 267 g/m~3,with an average of 71 g/m~3,the daily peak concentration of Humulus pollen was 672 g/m~3,with an average of 124 g/m3;④88.5% out-patients suffering from hay fever or asthma during Autumn were allergic to mugwort, 28.2% allergic to Humulus;⑤the grading of airborne Mugwort pollen concentration: 0～14 g/m~3 as grade 0, 15～40 g/m~3 as grade Ⅰ, 41～116 g/m~3 as grade Ⅱ,more than 117 g/m~3 as grade Ⅲ.Conclusion:It was the first time that Burkard volumetric sampler was employed in monitoring Mugwort and Humulus concentration in Beijing city. Average concentration of mugwort pollen was about 100 g/m~3, and concentration grade Ⅲ of mugwort pollen was distributed during the last ten days of August and the beginning of September.

Concentration of airborne pollen in Beijing city with burkard sampler / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the airborne pollen concentration in Beijing city during autumn and to grade the Artemisia pollen concentration.@*METHOD@#Burkard volumetric trap was used to sample the airborne pollen in Beijing city from August 1st 2007 to October 10th 2007. Patients only allergic to mugwort pollen were followed up, and their symptom scores and the mugwort pollen concentration were analyzed statistically.@*RESULT@#1) Mugwort and Humulus (including Cannabis Sativa L) were the main airborne pollen during August and September in Beijing city, accounting for 31% and 51%, respectively; 2) Mugwort pollen season was from 8th August to 8th October; 3) The daily peak concentration of Mugwort pollen was 267 g/m3, with an average of 71 g/m3, the daily peak concentration of Humulus pollen was 672 g/m3, with an average of 124 g/m3; 4) 88.5% out-patients suffering from hay fever or asthma during Autumn were allergic to mugwort, 28.2% allergic to Humulus; 5) the grading of airborne Mugwort pollen concentration: 0-14 g/m3 as grade 0, 15-40 g/m3 as grade I, 41-116 g/m3 as grade II, more than 117 g/m3 as grade III.@*CONCLUSION@#It was the first time that Burkard volumetric sampler was employed in monitoring Mugwort and Humulus concentration in Beijing city. Average concentration of mugwort pollen was about 100 g/m3, and concentration grade III of mugwort pollen was distributed during the last ten days of August and the beginning of September.

Study on aminoglycosides resistance and its resistance genes in multidrug resistant Acinetobacter baumannii / 国际检验医学杂志

Objective To investigate the resistance of multi-drug resistant Acinetobacter baumannii against aminoglycosides and its resistance genes.Methods The minimal inhibitory concentrations (MICs) of seven antimicrobials (gentamicin, amikacin, tobramycine, netilmicin, neomycin, streptomycin and kanamycin) against 20 strains of multi-drug resistant Acinetobacter baumannii were detected by agar dilution method. Meanwhile, two resistance genes of aminoglycoside nucleotidyltransferase were amplified by PCR and vertified by DNA sequencer.Results It was found that MIC50 and MIC90 of gentamicin, amikacin, streptomycin and kanamycin in 20 strains of Acinetobacter baumannii were all above 1 024 mg/L, and their resistant rates were 95%, 95%, 100% and 90% respectively, while the resistant rates of tobramycin, netilmicin and neomycin were 85%, 90% and 40% respectively. Two aminoglycoside-modification genes ant(2″)-Ⅰ and ant(3″)-Ⅰ were detected in 20 strains, with 55% and 80% of positive rate respectively. The double positive rate of two resistance genes was 50%.Conclusion The resistance of multi-drug resistant Acinetobacter baumannii against aminoglycosides was closely associated with ant(2″)-Ⅰ and ant(3″)-Ⅰ genes.

Monitoring of Concentration of Airborne Pollen with Burkard Sampler in Autumn,2007 in Beijing / 环境与健康杂志

Objective The aim of this study was to understand the pollen concentration and the daily variation during 2007 in Beijing by monitoring.Methods Burkard volumetric strap was used for the pollen sampling from August 1st to October 20th.Genus of pollen was identified by light microscope.Results Artemisia and Humulus.L pollen were the dominant airborne pollen in late summer and autumn in Beijing City.The average pollen concentration of Artemisia and Humulus.L were 71 grain/m3, 124 grain/m3.Both of them lasted from the August 8th until October 6th or 8th.Statistical analysis showed that the pollen concentration of Artemisia or Humulus.L at 2:00 pm to 4:00 pm was higher than that at 6:00 am to 8:00 am.Conclusion It is the first work to conduct the monitoring of the concentration of Artemisia and Humulus.L pollen with Burkard sampler in Beijing.Humulus.L is the dominant airborne pollen during August and September.

BETA-ADRENERGIC RECEPTOR BLOCKING EFFECT OF BUNOLOL: A COMPARISON WITH PROPRANOLOL / 中国药理学通报

The effect of bunolol on ?-adrenergic receptor of various tissues of animals were reported. The results showed that cumulative dose-response curves of isoprenaline were shifted paratlely to the right by bunolol ( 10-8, 10-7, 10-6mol/L ) on isolated rabbit atrium and guinea pig trachea preparation utilizing isoprenaline as a beta-receptor agonist. In the isolated rabbit right atrium preparation,the pA2 value of bunolol antagonizing the chronotropic action of isoprenaline was 9.3. In the isolated guinea-pig trachea preparation,the pA2 value of bunolol antagonizing the isoprenaline-induced relaxation was 9.1. By contrast, the 〔pA2 ( ?1 )- pA2 ( ?2 ) 〕 value of bunolol was 0.2, suggesting its ?-blocking action in the atrium being nearly 0,6 times more than that in the trachea. Also, comparison with pfopranolol, the blocking effect of bunolol on cardiac ?1 receptor and on trachea ?2 receptor were 4 times and 1.6 times more than that of propranolol respectively.Bunolol ( 1 mg/kg ) could, block completely the vasodilating response of epinephrine ( 10?gAg ) . At the same dosage, bunolol had no effect on blood pressure and heart rate of prior reserpini-sation rats.