Effects of flood stress on anthocyanins synthesis with relative enzymes and genes of Chrysanthemum morifolium cv. 'Hangju' / 中国中药杂志

The study is aimed at determine the content of anthocyanins and expressions of relative genes and activity of relative enzymes. The effects of flood stress on anthocyanins synthesis with relative genes and enzymes of Chrysanthemum morifolium cv. 'Hangju' were analyzed. The expression of CHS and the content of CHS presented the trend of first rising and after downward with the increase of flowering degree. The content of anthocyanins, the expression of DFR and the activity of DFR presented the trend of first downward and after rising with the increase of flowering degree. There was a positive correlation among anthocyanins,DFR gene and DFR. However there was no significant correlation among anthocyanins,CHS gene and CHS. Flood stress has significant effects on anthocyanins synthesis with relative genes and enzymes of Ch. morifolium cv. 'Hangju',but don't change the patterns of genes expression and anthocyanins and enzymes accumulation. DFR and DFR are the key gene and key enzyme of anthocyanins synthesis.

Study on medicinal materials quality of Thesium chinense in different branches / 中草药

Objective: To explore the effect of different amounts of branches on medicinal quality of T. chinense. Methods: Wild T. chinense taken from Jurong, Guli, and Maoshan was used as experimental materials. T. Chinense was divided into five levels according to the number of branches. The first level has 1-3 branches, 4-6 branches as second. The third level has 7-9 branches, 10-20 branches as fourth, and the fifth level has 20 or more branches. Quality of T. chinense was measured by growth indicators and effective components. Results: The quality of T. chinense in different levels was significantly different. The difference of dry weight of T. chinense could be up to 25 times, fifth level was 5.710 6 g, but the first level was only 0.224 5 g. The content of effective components and branch number were negatively correlated, flavonoids and kaempferol contents of T. chinense in level 1 were significantly higher than those in other levels which were 4.02% and 3.38 mg/g. In different branches levels of T. chinense, there was a significant positive correlation between flavonoids and kaempferol contents, but there was a significant negative correlation between kaempferol content and root crude of T. chinense. Conclusion: The number of T. chinense branches has a great impact on its quality, the more branches, the higher yield, and the lower content of active ingredient.

Study on winter dormancy of Thesium chinense and its phenological phase / 中国中药杂志

In order to explore reasonable artificial cultivation pattern of Thesium chinense, the biological characteristics and nutrients change in the process of winter dormancy of T. chinense was studied. The phenological period of T. chinense was observed by using fixed-point notation and the starch grains changes were determined dynamically by PAS-vanadium iron hematoxylin staixjing method. Soluble sugar and starch content were measured by anthrone-sulfuric acid method and amylase activity was determined by DN'S method. The results showed that the normal life cycle of T. chinense was two years. T. chinense was growing by seed in the first year, but growing by the root neck bud in the second year. During the process of dormancy, starch and soluble sugar could mutual transformation in different periods. T. chinense had sufficient carbohydrate to maintain growth and also a lot of small molecules to improve their ability to fight against adversity.

Identification of chemical signals and haustorium induced of Thesium chinense / 中国中药杂志

To separate and identify chemical signals which induce Thesium chinense haustorium formation, the components of T. chinense roots secretion collected with XAD-4 resin were detected by GC-MS. The effect of DMBQ as exogenous signals to induce haustorium formation in T. chinense was studied. Fifty-three compounds of 9 types had been detected, including hydrocarbons, esters, organic acids, ketones, alcohols, nitrogen containing compounds, phenolic acids, aldehyde and quinine. It is worth noting that the 2, 5-di-tert-butyl-1,4-benzoquinone has the core structure of 1,4-benzoquinone, which may play an important role in the parasitic relationship of Prunella vulgaris and T. chinense: DMBQ worked effectively on inducing haustoria, but induction effects vary widely in different concentrations. DMBQ with the concentration of 1 μmol x L(-1) showed the best effect of the inducing ability with a ratio of 110.52 when treated to induce haustoria.