Genetic polymorphism of cytokine genes in Han children in Beijing area / 中华实用儿科临床杂志

Objective To investigate the genetic polymorphism of 13 cytokines genes in Han individual children from Beijing area.Methods The polymerase chain reaction-sequence specific primers (PCR-SSP) method was used to determine cytokine gene polymorphism including interleukin (IL)-1α (-889C/T),IL-1β (-511C/T,+3942T/C),IL-1 R(pst1 1970C/T),IL-1RA(mspa1 11100T/C),IL-4Ra(+ 1902G/A),IL-12 (-1188C/A),interferon (IFN)-γ(+ 874A/T),transforming growth factor (TGF)-β1 (codon10C/T,codon25 G/C),tumor necrosis factor)(TNF)-α (-308G/A,-238G/A),IL-2 (-330T/G,+ 166G/T),IL-4 (-1098 T/G,-590 T/C,-33 T/C),IL-6(-174G/C,nt565G/A),IL-10 (-1082G/A,-819C/T,-592A/C) 13 cytokines genes in 210 unrelated healthy Han individuals from Beijing area.Results (1) Cytokine genes with frequencies more than or equal to 95 percent included IL-1β +3962 C,TGF-β Codon 25 G,IL-6-174 G,IL-6 nt565 G,TNF-α-308 G and TNF-α-238 G alleles;while cytokine genes with frequencies less than or equal to 5 persent included IL-1β + 3962 T,TGF-β Codon 25 C,IL-6-174C,IL-6 nt 565 A,TNF-α-308 A and TNF-α-238 A alleles.(2) The frequency of the high-producer-type GCC haplotype in IL-10 gene was 8.27％,while the low-producer-type ATA was 59.11％.The frequency of the highproducer-type TG haplotype in the TGF-β gene was 49.77％,while the low-producer-type CC was 0.The frequencies of the high-producer-types AG and AA haplotypes in the TNF-α gene were 3.34％,while those of the low-producer-types GG and GA were 96.67％.The frequency of the high-producer-types T/G genotype in the IL-2-330 gene was 11.90％,while those of the low-producer-types T/T and T/G were 88.10％.The frequencies of the high-producer-types G/G and G/C genotypes in the IL-6-174 gene were 99.52％,while that of the low-producer-types C/C was 0.48％.The frequency of the high-producer-types T/T genotype in the INF-γ+ 874 gene was 1.90％,and the frequency of the medium-producer-types T/A in the INF-γ+ 874 gene was 22.86％,while of the low-producer-types A/A was 75.24％.(3) New genotypes were found:the frequency of IL-2(-330,+ 166) GG/GT/TT genotype was 1.90％,and the frequency of IL-2(-330,+ 166) TG/GG/GT/TT genotype was 0.48％,The frequency of IL-6(-174,nt565) CG/CG genotype was 0.48％,the frequency of IL-6 (-174,nt565) GA/GG genotype was 1.90％,The frequency of IL-6 (-174,nt565) GG/GA/CA genotype was 0.48％,The frequency of IL-10(-1082,-819,-592) GCC/ATC/ATA genotype was 0.95％,The frequency of IL-10(-1082,-819,-592) ACC/ACA/ATC/ATA genotype was 35.71％,The frequency of IL-10 (-1082,-819,-592) GCC/ACC/ACA/ATC/ATA genotype was 0.48％.Conclusion The genetic polymorphism of cytokine genes in Han individuals from Beijing area is different from other areas of China with higher frequencies of IL-10 (-1082,-819,-592) ACC/ACA/ATC/ATA genotype.

Clinical research on treatment of migraine with pine needle moxibustion / 中国针灸

<p><b>OBJECTIVE</b>To observe the clinical effect and mechanism of migraine treated with pine needle moxibustion.</p><p><b>METHODS</b>Forty-three patients were randomly divided into 2 groups of pine needle moxibustion group (group A, 21 cases) and medicated thread moxibustion of Zhuang medicine group (group B, 22 cases). Same acu-points were selected as the main points for both groups, namely Fengchi (GB 20), Baihui (GV 20), Taiyang (EX-HN 5), Shuaigu (GB 8), Cuanzhu (BL 2), Sizhukong (TE 23), Yanglingquan (GB 34), Waiguan (TE 5) and Ashi points. Pine needle moxibustion was applied for the group A. Processed lighted pine needles were banded together with one even head to do moxibustion on acupoints or pain points. While, lighted medicated thread was adopted for the group B to do moxibustion. Therapeutic effects, the content of high-sensitivity C-reactive protein (hs-CRP), Mg2+ in the serum and the degree of pain of the two groups were compared.</p><p><b>RESULTS</b>(1) In the group A, the complete remission rate was 47.6% (10/21) and the effective rate was 90.5% (19/21). In the group B, the complete remission rate was 13.6% (3/22) and the effective rate was 50.0% (11/22). The two rates in the group A were superior to those in the group B (both P < 0.01); (2) In the group A, the content of hs-CRP was decreased from (4.29 +/- 0.98) mg/L to (2.11 +/- 0.87) mg/L, and Mg2+ was increased from (0.67 +/- 0.28) mmol/L to (1.07 +/- 0.16) mmol/L. In the group B, the contents of the above mentioned indices were respectively decreased from (4.30 +/- 1.07) mg/L to (3.38 +/- 1.15) mg/L and increased from (0.68 +/- 0.21) mmol/L to (0.88 +/- 0.25) mmol/L. There were significant differences between the two groups after treatment (both P < 0.05). (3) The degree of pain in the group A after treatment was much lower than that in the group B (P < 0.05).</p><p><b>CONCLUSION</b>The clinical effect of migraine treated with pine needle moxibustion is better than that with the medicated thread moxibustion of Zhuang medicine. And it has obvious regulation effect on the levels of hs-CRP and Mg2+ in the serum of patients.</p>

Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells / 中南大学学报(医学版)

OBJECTIVE@#To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells.@*METHODS@#HaCaT cells under UVA irradiation with 5J/cm(2) were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope.@*RESULTS@#Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P<0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells' ultrastructure.@*CONCLUSION@#Resveratrol can relieve the inhibition to HaCaT cell proliferation,injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.