Structure and degradation property of the PVA-collagen complex drug membrane / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the structure and degradation property of the polyvinyl alcohol (PVA)-collagen complex drug membrane.</p><p><b>METHODS</b>Drug collagen membrane was complexed with PVA. The physical and chemical properties of the membrane were characterized by transmission electron microscopy, scanning electron microscope, forier transform-infrared spectroscopy and differential scanning calorimetry. Degradation experiment was performed to determine the degradation property of membrane and a degradation curve was therefor drawn.</p><p><b>RESULTS</b>The thermodynamic stability of collagen membrane was not destroyed by adding PVA. Collagen had good compatibility with PVA. Compared with collagen membrane, collagen-PVA complex membrane had smaller and evener pores. Adding PVA decreased the degradation rate of membrane.</p><p><b>CONCLUSIONS</b>PVA-collagen membrane has better microstructure and antidegradation property than collagen membrane.</p>

The construction of two kinds of artificial bone and the comparison of their osteogenesis capability in vivo / 中国医学科学院学报

<p><b>OBJECTIVE</b>To construct artificial bone with collagen-hydroxyapatite (HA) or collagen-HA-chondroitin sulfate (CS) as the scaffolds, and observe their biological properties.</p><p><b>METHODS</b>The artificial bones were constructed by attaching recombinant human bone morphogenetic protein 2 (rhBMP-2) on those scaffolds. And then they were embedded into muscles of rats. Every weekend those newly formed bones were taken from muscles for comparing the difference in osteogenetic capability of two kinds of artificial bone in vivo.</p><p><b>RESULTS</b>Both kinds of artificial bones could induce bone regeneration in muscle. The collagen-HA-CS artificial bone was superior to the collagen-HA artificial in bone-guided degree.</p><p><b>CONCLUSIONS</b>The CS could promote the form action of new bone and accelerate the bone healing.</p>

Construction of a collagen gel dermis with improved biological property / 中国医学科学院学报

<p><b>OBJECTIVE</b>To improve the biological property of artificial skin.</p><p><b>METHODS</b>We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.</p><p><b>RESULTS</b>The neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).</p><p><b>CONCLUSION</b>The collagen gel dermis is an useful dermis substitute.</p>