Simultaneous determination and correlation study on the active constituents in Salvia miltiorrhiza Bge / 中成药

AIM:To establish a simple and accurate HPLC method for quality evaluation and determination of 8 active constituents, i.e. Danshensu, Protocatechualdehyde,rosmarinic acid,Salvianolic acid B, Dihydrotanshinone, Cryptotanshinone, Tanshinone Ⅰand Tanshinone ⅡA in Radix Salviae Miltiorrhizae, at the same time, in order to study the correlations of the contents of the 8 constituents. METHODS: Detected at 286 nm, analytes were separated on a ODS C_ 18 column(150 mm?4.6 mm, 10 ?m) eluted gradually with methanol and 1% tetrafuran in 1% formic acid water solution(3∶97)on the increasement of methanol from 3% to 70% in the whole analysis. After determination, SPSS 13.0 software was used to calculate the correlation between the 8 constituents. RESULTS: Under the above conditions, HPLC method was constructed to quantify 8 constituents,and their correlation coefficients were calculated. CONCLUSION: The result shows that the contents of the 8 constituents in Salvia miltiorrhiza Bge. vary greatly from different origins; and there are significant correlations between the contents of hydrophilic constituents such as Salvianolic acid B,Danshensu,rosmarinic acid, so do hydrophobic constituents like Cryptotanshinone,Tanshinone Ⅰ,Tanshinone ⅡA,but there is no correlation between the hydrophilic constituents and hydrophobic constituents.

Best harvest time of Isatis indigotica Fort / 中成药

AIM: To study the best harvest time of Isatis Indigotica Fort.. METHODS: The adenosine content was determined by HPLC, and at the same time, the dry substance accumulation, the alcohol extract of the herb were also compared at the different growth periods of Isatis indigotica Fort.. RESULTS:During its whole growth period, the contents of adenosine and alcohol extract increase and very fast before November. The dry substance accumulates highly in December. CONCLUSION: In consideration of the yield and the contents of adenosine and alcohol extrat, the best harvest time should be between November and December.

Study on fingerprint for the quality control of Banlangen Granule / 中成药

AIM: To establish a fingerprint analytical method for ensuring the quality of Banlangen Granule (Isatis indigotica Fort). METHODS: According to pre column derivatization HPLC method, gradient elution with sodium acetate buffer (pH6.4) acetonitrile (95∶5) in a BDS C 18 column, was applied to detect amino acid at UV 360nm; and the coefficients of cosine of include angle and distance were employed to judge the quality similarity of the samples. RESULTS: The HPLC method is accurate and reproducible and makes almost all the peaks separated; and the two coefficients can show the similarity of each sample. CONCLUSION: The developed method is simple, accurate and operable and can efficiently control the quality of Banlangen Granule.

Effect of Different Solvents on Extraction Efficiency of Imperialine / 中成药

Objective: To compare the extraction efficiency of imperialine extracted with different solvents. Methods: The content of imperialine in the extracts was determined by thin layer chromatography scanning. Results: Among the methods suitable for alkaloid extraction, the solvent ether chloroform 95% ethanol (25∶8∶2.5) is the best for imperialine extraction. Conclusion: The imperialine extraction efficiency is greatly affected by the extraction solvent.