RESUMO
Objective: To provide information for strenthening the management of antibacterials through an investigation of the clinical use of antibacterials in the Department of Pediatrics in the people's hospital of Haiyan. Methods: A retrospective review of the medical records of all the inpatients discharged from the Department of Pediatrics of the hospital during the period of January 1999 to December1999 was carried out regarding the application of antibacterials for them. Results: Antibacterials were used in 95.76% of the patients, with a period averaged 3.8 days. The most frequently used three antibacterials were cephalosprin, aminoglycoside and macrolides. The rate of unreasonable use of antibacterials was 34.55%. Antibacterials selected on basis of susceptibility tests was 20.61%. The main administration route was intravenous drip. Conclusion: In order to prevent drug resistance of the bacteria and decrease adverse effects of antibacterials, it was very important to strenthen management of reasonable use of antibacterials and application of susceptibility tests and raise the awareness of the side effects of antibacterials on children.
RESUMO
Endothelin (ET) is an endothelium-derived vasoconstrictive peptide. We have developed a sensitive and selective radioimmunoassay for porcine/human endothelin (ET-1). 126I-labled ET was perpared by lodogen method and was purified by HPLC. Its specific activity was about 64.75 TBq(1750Ci)/mmol. The assay has a detection limit of 0.17pg/tube and the assay range was 0.25-1000pg/tube. The procedure was developed for extraction of endothelin from human plasma using C18 Sep-pak extraction cartridges. Human plasma samples were extracted, assayed and the plasma ET values of 17 healthly volunteers was found to be 2.81 ?0.60pg/ml. Both patients with uraemia(n = 20) and acute myocardial infarction(n= 11) had significantly higher plasma values than normal subjects.