Relationship between breakfast eating frequency and health-risk behavior of high school students in Chongqing / 中国学校卫生

Objective@#To investigate the relationship between breakfast eating frequency and health-risk behavior of high school students in Chongqing, and provide a reference for conducting students to get a good habit of eating breakfast everyday.@*Methods@#A total of 3 283 high school students in Chongqing were selected to participate in a questionnarie by using convenience sampling method. Logistic regression analysis were condueted to analyze the influences made by the unhealty eating behaviors on eating frequency of breakfast.@*Results@#There were 2 064(69.2%) students eating breakfast everyday, 1 219(37.1%) students having no breakfast or eating breakfast sometimes. There were significant differences between different gender, grade, native place, drinking energy drink, smoking, drinking alcohol, gambling, internet addiction students rate of unhealthy breakfast eating frequency(χ2=6.80, 53.97, 8.10, 23.79, 7.89, 16.31, 3.93, 17.96, P<0.05), the males (36.1%) was beyond the females(33.3%), the energy drink drinkers(41.2%) was beyond the non-drinkers(31.7%), the smokers (40.9%) was beyond the non-smokers(34.4%), the alcohol drinkers(40.3%) was beyond the non-drinkers(33.3%), the gamblers(39.7%) was beyond the non-gamblers(34.3%), those having internet addictions (40.0%) was beyond those having no addictions(33.0%). Drinking energy drink(OR=1.32, 95%CI=1.12-1.55), drinking alcohol(OR=1.24, 95%CI=1.02-1.51), internet addiction(OR=1.42, 95%CI=1.19-1.68) were positively correlated with unhealthy breakfast eating frequency of high school students(P<0.05).@*Conclusion@#Social, families and schools should strengthen the guidance of high school students to develop the habit of eating breakfast every day, and advoid the clustering of skipping breakfast and health-risk behaviors.

Establishment and differential protein identification of two-dimensional gel electrophoresis for proteomics in the spinal cord of morphine-tolerant rats / 中南大学学报(医学版)

To establish a two-dimensional gel electrophoresis (2-DE) map for comparative proteomic analysis of rat spinal cord with chronic morphine tolerance, and to detect differentially expression proteins that are associated with chronic morphine tolerance.
 Methods: Sixteen male SD rats received the intrathecal catheterization operation and they were randomly divided into a morphine tolerance group (MT group, n=8) and a saline group (NS group, n=8). The lumbar enlargement segments of the MT group and the NS group spinal cord were harvested and proteins were separated by 2-DE. Differential proteome profiles were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The 2-DE maps were visualized after coomassie blue staining and analyzed using PDQuest analysis software. Identification of differential protein spots was conducted by MALDI-TOF-MS, and the Mascot query software was used to search Swiss-Prot database for bioinformatics analysis. Western blotting was used to verify the expression of some differentially expressed proteins.
 Results: A total of 1 000 spots were identified in 2-DE maps of rat spinal cord tissues from the MT group and the NS group, and 36 proteins were significantly differentially expressed in the MT group compared with the NS group. Identification was conducted by MALDI-TOF-MS and Swiss-Prot database through Mascot query software, and a total of 14 proteins were obtained. Among them, 2 protein spots were down-regulated in the MT group compared with that in the NS group, and 12 protein spots were up-regulated in the MT group compared with that in the NS group. Two kinds of proteins (NUDAA, ENOG) were verified by Western blotting and the results were consistent with proteomics data.
 Conclusion: The optimized 2-DE profiles for the proteome of spinal cord tissue in rats with chronic morphine tolerance is established preliminarily, which showed that morphine tolerance can cause changes in the expression of various proteins in the spinal cord.

Effects of Sex and Age on the Brainstem Auditory Evoked Potentials(BAEP) / 中国医师杂志

Objective To study the effects of sex and age on the Brainstem Auditory Evoked Potentials(BAEP).Methods 53 normal subjects' BAEP(106 ears) were examed.Multiple liner correlation and stepwise regression were used for statistical analysis.Results Wave Ⅲ latency and Ⅰ-Ⅲ,Ⅲ-Ⅴ interpeak latencies had correlation with age.All latencies and interpeak latencies(except Ⅰ-Ⅲ) had correlation with sex.All latencies and interpeak latencies were shorter in female than in male,for wave Ⅴ,this phenomenon was particularly obvious.Wave Ⅴ latency was about 0.2ms shorter in female than that in male.Conclusions Sex and age are major physiological factors on BAEP.If we pay colse attention to them in clinical application,the diagnosis is more objective and accurate.