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@#The aim of this study was to prepare albumin nanoparticles by thermal driven self-assembly, and to investigate the formation mechanism, cellular uptake, the kinetics of cellular uptake and intracellular degradation, etc. By measuring the concentrations of thiol, amino and carboxyl groups, the formation mechanism of albumin nanoparticles was revealed. CCK-8 assay was performed to detect the cytotoxicity; inverted fluorescence microscope was used to observe the cellular uptake of the nanoparticles; while the fluorescence resonance energy transfer(FRET)method was applied to investigate the cellular uptake and intracellular degradation kinetics. The drug-loading capacity was investigated using paclitaxel(PTX)as the model drug. The results showed that the albumin nanoparticles produced by thermal driven self-assembly were safe, nontoxic, biodegradable and stabilized by intermolecular disulfide and amide bonds. The drug-loading study indicates that PTX can be highly encapsulated in the nanoparticles. Hence, thermal driven self-assembly method is green and easy to operate, and the albumin nanoparticles can be applied as a new delivery platform for anticancer drugs.
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Objective To study the dose of IVGG in treating atypical Kawasaki disease(KD) at defervescence and the necessity.Methods Clinical data of 54 cases of atypical Kawasaski disease without coronary artery lesion (CAL) but with the abatement of fever in 10 days were retrospectively analyzed. They were divided into there groups according to IVGG dosage ;group A (1g/kg IVGG n=11 ),group B(2g/kg n=24 ) and group C (blank n=19 ) .CAL was observed to at 14~21 days and 0.5year. Results Compared with group A and B, The rate of CAL was significantly higher (P0.05) . Conclusions In order to prevent CAL ,atypical Kawasaki disease(KD) children with confirmed diagnosis in 10 days should all be treated with IVGG whether with abatement of fever or not .The dose of 1g/kg IVGG would have satisfactory effects on atypical KD children at defervescence.