RESUMO
Object To study the isolation and purification of a polysaccharide, obtained from Paecilomyces tenuipes Samson, its molecular weight, sugar composition, and mode of linkage Methods Crude polysaccharide was extracted by water at ambient temperature and purified on Sephadex G 100 column Its monosaccharide composition was determined by ionic ion exchange column after complete hydrolysis with acid Their mode of linkage was determined by methylation and glycosidic linkage established by IR and NMR spectra Results HPLC spectrum showed that the polysaccharide was of homogeneous composition, which was also proved latter by GC MS and NMR Conclusion Polysaccharide obtained from P tenuipes Samson is ? (1→6) linked and composed of only D glucose The molecular weight was 2 05?10 4