RESUMO
Purpose To evaluate the effect of ergosterol combined with risedronate on fracture healing. Methods Sixty male Sprague Dawley fracture model rats were assigned into group A (n=20), group B (n=20), and group C (n=20) at random. All rats were fed by gavage until their sacrifice as it follows: group A with ergosteroside and risedronate, group B with risedronate, and group C with saline solution. At weeks 2 and 4, 10 rats of each group were sacrificed. Healing effect and bone tissue changes in the fractures site were assessed by using hematoxylin and eosin stain histology. Enzyme-linked immunosorbent assay was used to detect the expression of serum bone morphogenetic protein-2 (BMP-2), bone morphogenetic protein-7 (BMP-7), and vascular endothelial growth factor (VEGF). Reverse transcriptase polymerase chain reaction was applied to detect the expression of osteoprotegerin (OPG) mRNA, osteocalcin (OCN) mRNA and core-binding factor subunit-?1 (CBF-?1) mRNA. Results In terms of serum BMP-2, BMP-7, and VEGF expression at weeks 2 and 4 after gavage, group A < group B < group C (P<0.05). At week 4 after gavage, serum VEGF expression in the three groups harbored positive relationship with serum BMP-2 and BMP-7 expression (P<0.05). Regarding serum OPG, OCN and CBF-?1 mRNA expression at weeks 2 and 4 after gavage, group A Assuntos
Masculino
, Animais
, Ratos
, Consolidação da Fratura/efeitos dos fármacos
, Ergosterol/análise
, Fator A de Crescimento do Endotélio Vascular
, Osteoprotegerina/isolamento & purificação
, Ácido Risedrônico/análise
, Reação em Cadeia da Polimerase Via Transcriptase Reversa
RESUMO
Objective To study the clinical efficacy and safety of arthroscopic minimally invasive surgery applied in tibial plateau fracture. Method 50 patients with tibial plateau fracture from Jan 2014 to Jan 2016 were randomly divided into control group (traditional surgery) and observation group (arthroscopic minimally invasive surgery) according to the random number method, 25 patients in each. The surgery condition, postoperative complications and function recovery of knee joint were observed and compared. Results Compared with the control group, the operation time, intraoperative blood loss, ambulation time, weight-bearing ambulation time, and postoperative drainage volume were significantly decreased in observation group with statistical difference (P < 0.05); compared with the control group, the incidence of postoperative complications such as postoperative wound infection, malunion, traumatic arthritis, knee joint stiffness was obviously reduced in the observation group with statistical difference (P < 0.05); compared with the control group, the excellent rates of knee joint function score and radio-graphic score were significantly increased in the observation group with statistical difference (P < 0.05). Conclusion The arthroscopic minimally invasive surgery can significantly reduce the incidence of postoperative complications for patients with tibial plateau fracture and is beneficial to the recovery of knee joint function with higher clinical efficacy and treatment safety.
RESUMO
Objective To study the clinical efficacy and safety of arthroscopic minimally invasive surgery applied in tibial plateau fracture. Method 50 patients with tibial plateau fracture from Jan 2014 to Jan 2016 were randomly divided into control group (traditional surgery) and observation group (arthroscopic minimally invasive surgery) according to the random number method, 25 patients in each. The surgery condition, postoperative complications and function recovery of knee joint were observed and compared. Results Compared with the control group, the operation time, intraoperative blood loss, ambulation time, weight-bearing ambulation time, and postoperative drainage volume were significantly decreased in observation group with statistical difference (P < 0.05); compared with the control group, the incidence of postoperative complications such as postoperative wound infection, malunion, traumatic arthritis, knee joint stiffness was obviously reduced in the observation group with statistical difference (P < 0.05); compared with the control group, the excellent rates of knee joint function score and radio-graphic score were significantly increased in the observation group with statistical difference (P < 0.05). Conclusion The arthroscopic minimally invasive surgery can significantly reduce the incidence of postoperative complications for patients with tibial plateau fracture and is beneficial to the recovery of knee joint function with higher clinical efficacy and treatment safety.
RESUMO
Summary: The aim of this study was to determine if the potassium aspartate and magnesium (PAM) prevent reperfusion-induced ventricular arrhythmias (RIVA) in ischcmia-reperfusion (IR) rabbit heart. Thirty rabbits were randomly divided into control, ischemia and PAM groups. Arterially-perfused rabbit left ventricular preparations were made, and transmural ECG as well as action potentials from both endocardium and epicardium were simultaneously recorded in the whole process of all experiments. In control group rabbit ventricular wedge preparations were continuously perfused with Tyrode's solution, and in ischemia group and PAM groups the perfusion of Tyrode's solution was stopped for 30 min. Then the ischemia group was reperfused with Tyrode's solution and the PAM group with Tyrode's solution containing 2.42 mg/L PAM, respectively. ECG, QT interval, transmural repolarization dispersion (TDR) and action potentials from epicardium and endocardium were simultaneously recorded, and the RIVA of the wedge preparation was observed. Compared with control group, TDR and incidence of RIVA were significantly increased in ischemia group (P<0.05). The incidence of RIVA in control, ischemia and PAM group was 0/10, 9/10 and 1/10, respectively. Compared with ischemia group, TDR and incidence of RIVA were significantly reduced in PAM group (P<0.05). Potassium aspartate and magnesium significantly reduce TDR and prevent ventricular arrhythmia in ischemic rabbit heart.