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Objective:In the era of precision medicine, there is an urgent need for a preclinical evaluation method with a high cost-benefit ratio to improve the effectiveness and value of clinical trials.Methods:Taking clinical needs and scientific research purposes as the starting point, the platform focused on four aspects of project management, information retrieval, quality control, and practical application, and introduced in detail the management practice of building a patient-derived xenograft model platform system.Results:With the support of the institutional system, quality control system, and information system, the patient-derived xenograft model platform was formed with standardization as the core. With the assistance of this platform and scientific research management, as of December 2021, there are 48 animal models of patient-derived xenograft in the database. In total of 6 SCI scientific and technological articles were published using these animal models, with a total impact factor of 36.77 (the highest single article was 7.333). In total of 6 direct industrial projects, 6 clinical trial-related projects, and 4 NSFC projects were approved with a total research fund of 1.5 million yuan.Conclusions:Continuous construction and improvement of the existing platform will help promote the development of basic research translation and clinical research in the field of oncology, and accelerate the development of new oncological diagnosis and treatment models, thereby benefiting more patients.
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【Objective】 To investigate the resource allocation status of blood testing laboratories in 14 blood stations in Gansu Province, explore the impact of differences in basic conditions on the comprehensive testing ability of laboratories, so as to promote the homogenization and standardization of blood screening capacity in blood stations in Gansu and improve blood safety and effectivenes. 【Methods】 An evaluation index system of laboratory resource allocation was constructed and a question-naire was designed. The data of human resources, infrastructure and key equipment of 14 blood stations were collected. The entropy weight -TOPSIS method was used to evaluate and rank the resource allocation of 14 blood stations. 【Results】 In the comprehensive evaluation of blood testing laboratory resource allocation in 14 blood stations in Gansu, the top three were laboratories A, B and I, and the last three were laboratories G, M and J. On the whole, the main issue was unreasonable structure of human resources: most laboratories had unreasonable age structure; except for Laboratory A, there was no personnel with bachelor's degree or above in laboratories; most laboratories had not established a team with intermediate professional titles. In terms of infrastructure, the size of seven laboratories could not meet the needs of modern laboratory testing, and all eight blood stations had no spare nucleic acid laboratories nor a mutual spare laboratory with other blood stations As for the key equipment, 5 laboratories had no automatic blood grouping diagnostic instrument, 5 laboratories only had one set of enzyme immunoassay detection system, 3 laboratories had no spare equipment for the key equipment, which means if the equipment failure could not be repaired in time, the release of results would be affected. 【Conclusion】 There were significant differences in human resources, infrastructure and key equipment of blood testing laboratories in 14 blood stations in Gansu, which had a great impact on laboratory testing capacity and subsequent development. It is suggested that governments at all levels and health administrative departments optimize the input of laboratory resource allocation according to the blood collection volume of blood stations to gradually narrow the differences in resource distribution between different regions, improve the degree of laboratory automation and optimize the personnel structure, so as to build high-quality and efficient blood testing laboratories and ensure the safety of clinical blood use.
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Objective:To construct the follow-up index system for lymphoma survivors, so as to provide a basis for survivors to obtain effective care during the follow-up period and to explore the follow-up pattern of cancer survivors in our country.Methods:From April 2021 to April 2022, the first draft of the follow-up index system for lymphoma survivors was developed through literature search and semi-structured interviews. Delphi method and Analytic Hierarchy Process were used to determine the quality of index system and index weight.Results:Twenty experts were consulted for 2 rounds. The recovery rate of the two rounds of expert letter inquiry was 100%, The authoritative coefficients were 0.938, 0.960. The Kendall′s W value were 0.150, 0.284 and 0.246 respectively with good coordination degree ( χ2=9.00, 85.29, 240.69, all P<0.05). Finally, the follow-up index system for lymphoma survivors was constructed, which included 4 first-level indicators, 16 second-level indicators and 49 third-level indicators. Conclusions:The follow-up index system for lymphoma survivors was scientific and can guide nurses to develop personalized follow-up plans and standardized implementation.
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Objective:To analyze the application effects of anchored instruction combined with problem-based learning (PBL) in the clinical teaching of department of gynecology.Methods:Taking the implementation time (June 2020) of anchored instruction combined with PBL in the hospital, 86 clinical interns who entered the department of gynecology before the implementation (from April 2019 to May 2020) were included in control group, and this group adopted traditional teaching method; 82 interns enrolled after the implementation (between June 2020 and December 2020) were selected as observation group and were given new teaching method. The theoretical assessment results, practical operation results, independent learning ability and teaching satisfaction were compared between the two groups after 1 month of training. SPSS 22.0 was used for t test and rank sum test. Results:After 1 month of training, the scores of theoretical assessment results [(81.20±10.64) vs. (73.96±8.25)] and mini-clinical evaluation exercise (Mini-CEX) scale of observation group were significantly higher than those of control group, and the independent learning ability and teaching satisfaction evaluation were significantly better than those of control group ( P<0.05). Conclusion:Anchored instruction combined with PBL can effectively improve the independent learning ability and learning satisfaction of interns in department of obstetrics and gynecology, with good application effects.
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Objective To investigate the current state of psychological distress of lymphoma patients'caregivers, and analyze its influencing factors. Methods From October 2017 to February 2018, 96 pairs (patients and their caregivers) from the department of lymphoma were enrolled. Distress Thermometer and self-designed general scale were adopted to conduct survey. Results The psychological distress score of lymphoma caregivers was as high as (4.76 ± 2.28) points, which was significantly higher than the patient's score (3.81±2.33) points, the difference was statistically significant (t=4.070,P<0.05 ). Different ages, economic status, and patient's disease of caregivers had different psychological distress, and the difference was statistically significant (P<0.05). Meta-linear regression analysis showed that age and patient psychological distress were the main influencing factors of psychological distress of lymphoma patients'caregivers, which could explain the variability of 32.7％(P<0.05). Conclusions The psychological distress of lymphoma patients'caregiver is at a moderate level, which is affected by their age and psychological distress of the patients. It suggests that clinical medical staff should pay attention to the psychological distress of patients and strengthen the psychological support of caregivers to better promote patient care.
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Objective@#To investigate the effect of symptom diary on the self-management ability of lymphoma patients.@*Methods@#A convenient sampling method was adopted to select 57 cases of lymphoma patients who received chemotherapy from February to October 2018 as the study subjects. According to the order of admission, they were divided into the intervention group (n = 25) and the control group (n = 32). The control group received the lymphoma department chemotherapy nursing routine; The intervention group applied symptom diary intervention on the basis of routine nursing, and evaluated the self-management ability of patients in the two groups before the first cycle and after the sixth cycle of chemotherapy, respectively.@*Results@#There was no significant difference in the scores of self-management ability between the two groups before intervention (P>0.05). The self-management scores of the two groups were both improved before and after the intervention, and the intervention group was higher than the control group. The difference was statistically significant (P<0.05); after intervention, for total self-management ability and the four dimensions of daily life management, symptom management, medical staff communication, and information management. the intervention group scored (4.16±0.28), (4.31±0.44),(4.31 ± 0.44), (4.38 ± 0.48), (3.96 ± 0.60) points, significantly higher than the control group (3.85 ± 0.45), (3.96 ± 0.45), (3.96 ± 0.57), (3.64 ± 0.77), (3.26±0.68) points, the difference was statistically significant (t=2.235-4.175, P<0.05).@*Conclusion@#The application of symptom diary can effectively improve the self-management ability of patients with lymphoma during chemotherapy, stimulate the ability of patients to cope with disease, improve the quality of life of patients, and promote rapid recovery.
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Objective To investigate the effect of symptom diary on the self-management ability of lymphoma patients. Methods A convenient sampling method was adopted to select 57 cases of lymphoma patients who received chemotherapy from February to October 2018 as the study subjects. According to the order of admission, they were divided into the intervention group (n=25) and the control group (n=32). The control group received the lymphoma department chemotherapy nursing routine; The intervention group applied symptom diary intervention on the basis of routine nursing, and evaluated the self-management ability of patients in the two groups before the first cycle and after the sixth cycle of chemotherapy, respectively. Results There was no significant difference in the scores of self-management ability between the two groups before intervention (P>0.05). The self-management scores of the two groups were both improved before and after the intervention, and the intervention group was higher than the control group. The difference was statistically significant (P<0.05); after intervention, for total self-management ability and the four dimensions of daily life management, symptom management, medical staff communication, and information management. The intervention group scored (4.16±0.28) , (4.31±0.44) ,(4.31 ± 0.44), (4.38 ± 0.48), (3.96 ± 0.60) points, significantly higher than the control group (3.85 ± 0.45), (3.96 ± 0.45), (3.96 ± 0.57), (3.64 ± 0.77), (3.26±0.68) points, the difference was statistically significant (t=2.235-4.175, P<0.05). Conclusion The application of symptom diary can effectively improve the self-management ability of patients with lymphoma during chemotherapy, stimulate the ability of patients to cope with disease, improve the quality of life of patients, and promote rapid recovery.
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104 successful treated amblyopes were screened from the ophthalmology outpatient departmt,who had successfully amblyopia treatment,all with the corrective visual acuity around 0.8 and the visual acuity of two eyes were less than two lines.Titmus stereogram was used to test stereoacuity,the chi-square test was used to analyze the results.There was statistical significance between the three types of amblyopia(x2 =11.294,P ＜ 0.05),individuals with ametropic amblyopia have better stereoacuity than others,individuals with anisometropic amblyopia and strabismic amblyopia have worse stereoacuity than others;There was statistical significance between different degrees of amblyopia (x2 =13.455,P ＜ 0.05),individuals with mild and moderate amblyopia have better stereoacuity than others,individuals with severe amblyopia have worse stereoacuity than others;There was statistical significance between differentdiagnosed age (x2 =11.263,P ＜ 0.05),the first diagnosed age less than 7 years old was better than over 7 years old;No difference was identified between thestereoacuity of monocular and binocular amblyopia.The visual acuity of different kinds of amblyopia could recover to normal level after regular amblyopia treatment,however,the stereoacuity has not recovered to normal level,it is necessary to take exercise to improve binocular function.As a result,the stereoacuity is considered to be a standard to evaluate the effect of amblyopia treatment.
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Background The histopathological and functional changes are affected by age,gender and axial length (AL).However,whether these factors affect the volume and thickness of various layers of retina is not elucidated.Understanding macular intraretinal layer volume changes and influence factors can offer useful information for relevant clinical study.Objective This study was to measure the influence of age,gender and AL on the volume of individual retinal layers at macula zone with optical coherence tomography (OCT) in normal population.Methods A cross-sectional study was carried out under the approval of Ethic Committee of Tongling People's Hospital.One hundred and thirty-four eyes of 92 healthy individuals who received physical examinations and ocular examinations by slit lamp microscope,90 D pre-set lens,RM-8000A computer automatic optometry and non-contact tomometer were recruited from April to July 2016.The individuals were grouped based on gender or ages (7-14 years group,15-49 years group,50-80 years group).The retinal thickness and volume of different layers within 6 mm circle area around fovea were measured by Spectral-OCT with Posterior Pole mode and processed by the layered software.AL was measured with ODM-2100 A/B ultrasonic apparatus.Results The volume values of whole retinal thickness (WRL),retinal nerve fiber layer (RNFL),inner nuclear layer (INL) and outer plexiform layer (OPL) in males were (8.80 ± 0.38),(0.92 ± 0.09),(1.03 ± 0.07) and (0.79 ± 0.07) mm3,which were significantly increased in comparison with (8.66±0.34),(0.88±0.07),(1.00±0.07) and (0.77±0.06)mm3 in females (P=0.025,0.027,0.012,0.030).The WRL,GCL and IPL volume values were largest in the 15-49 years group and least in the 7-14 years group,and the volume values of RNFL,outer nuclear layer (ONL),retinal pigment epithelium (RPE) and photoreceptors (PC) were significantly lower in the 7-14 years group and 50-80 years group than those in the 15-49 years group (all at P ＜ 0.01).The AL was negatively correlated with WRL,GCL and IPL volumes (r=-0.226,-0.385,-0.373,all at P＜0.01).Conclusions The retinal volume value at macular area is larger in men than that in women.The retinal volume area shows firstly increased and then decreased during aging.The AL shows a negative correlation with the macular volume.
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Objective To explore related symptom clusters of patients with lymphoma receiving chemotherapy, and to investigate the categories and influencing factors. Methods From the period of September to December 2016, 111 inpatients with lymphoma receiving chemotherapy were enrolled from the department of lymphoma. Anderson Syptom Assessment Scale Chinese version (MDASI-C), Self-designed general scale and ECOG physical status score were adopted to conduct survey. Results During chemotherapy, the first three syndromes with the highest incidence and most severe were dry mouth, sleep disturbed and fatigue, their incidence rates were 79.3%(88/111), 75.7%(84/111) ,73.0%(81/111), the severity degree was 2(1-4), 2(1-4), 2(0-5). By exploratory factor analysis, three syndrome clusters were found out: sleep-gastrointestinal symptom group, fatigue-psychological symptom group and disease symptom group. Multiple linear regression analysis showed that the influencing factors of the digestive tract symptom group were gender, number of chemotherapy. The fatigue-psychological symptoms were sex, ECOG score, marriage. The disease symptom group was timess of chemotherapy (t=-2.326-3.917, P<0.05 or 0.01). Conclusions The incidence of related syndromes in patients with chemotherapy is high, some constitute syndrome clusters. Gender, marriage, ECOG score, number of chemotherapy are related factors of the syndrome clusters.
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Objective To explore related symptom clusters of patients with lymphoma receiving chemotherapy, and to investigate the categories and influencing factors. Methods From the period of September to December 2016, 111 inpatients with lymphoma receiving chemotherapy were enrolled from the department of lymphoma. Anderson Syptom Assessment Scale Chinese version (MDASI-C), Self-designed general scale and ECOG physical status score were adopted to conduct survey. Results During chemotherapy, the first three syndromes with the highest incidence and most severe were dry mouth, sleep disturbed and fatigue, their incidence rates were 79.3%(88/111), 75.7%(84/111) ,73.0%(81/111), the severity degree was 2(1-4), 2(1-4), 2(0-5). By exploratory factor analysis, three syndrome clusters were found out: sleep-gastrointestinal symptom group, fatigue-psychological symptom group and disease symptom group. Multiple linear regression analysis showed that the influencing factors of the digestive tract symptom group were gender, number of chemotherapy. The fatigue-psychological symptoms were sex, ECOG score, marriage. The disease symptom group was timess of chemotherapy (t=-2.326-3.917, P<0.05 or 0.01). Conclusions The incidence of related syndromes in patients with chemotherapy is high, some constitute syndrome clusters. Gender, marriage, ECOG score, number of chemotherapy are related factors of the syndrome clusters.
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To analyze the effect of pre-surgical status on the post-surgical stereopsis in surgically aligned adult con-comitant strabismus.By using Titmus and Randot stereo tests,we measured near stereo acuity of 99 adults who had constant exotropia and were successfully aligned by surgery.A covariance analysis showed that the post-surgical stereo acuity was significantly determined by the type of strabismus (i.e.,whether that's intermittent or not;P =0.001),the duration of pre-surgical exotropia (P =0.002)and the year on-set (P =0.048)but not by the pre-surgical deviation (P =0.133).We conclude that the stereopsis of adult concomitant strabismus can be reestab-lished by surgery,while the extent of such an improvement is determined by the type of strabismus,the duration of pre-surgical exotropia and the year on-set of patients.
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A total of 60 myopia patients (120 eyes) who were going to undergo myopia LASIK were selected in this study. The patients were divided into two groups of anisometropia and non-anisometropia,each group had 30 patients (60 eyes). Checked vision, fusion range,near and distant stereoscopic at 10 day pre-operation and at 1,3 and 6 month in post operation. After the LASIK, all of 60 patients' anisometropia were significantly reduced, binocular stereopsis were obviously improved than pre-operation, especially the anisometropia group; the binocular fusion range also got better,but there was no significant difference between the two groups. This study clarified that the visual function of anisometropia could be improved after LASIK.
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Objective To investigate the correlation between the positive rate of PCR-reverse dot blot genotyping test and the loads of the viral nucleic acid. Methods The fluorescent PCR assay was used to detect the high-risk HPV(HR-HPV)DNA loads in the cervical mucus samples from 1162 female pa-tients. Patients with positive HR-HPV DNA were further analyzed by PCR-reverse dot blot hybridization as-say for HPV genotyping. Results The overall positive rate of genotyping test was 68. 8% . The positive rate of genotyping test had a significant positive correlation with the Log Koc of HR-HPV DNA loads(r=0. 944, P﹤0. 01). The quadratic curve fitting formula was Y= -1. 806+0. 558X-0. 031X2(Y for genotyping positive rate,X for Log Koc of HR-HPV DNA loads). There were significant differences with the positive rate of gen-otyping test among patients with different viral loads(P﹤0. 01). When HR-HPV DNA loads were 104-105 copies/ ml,105-106 copies/ ml,106-107 copies/ ml and more than 107 copies/ ml,the positive rate of HPV genotyping test were 27. 8% ,48. 5% ,74. 0% ,97. 5% and 33. 3% ,51. 5% ,78. 0% ,97. 5% respective-ly by using different genotyping detection reagents. Conclusion The positive rate of PCR-reverse dot blot genotyping test was correlated with the loads of HPV nucleic acid.
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Objective To study the clinical features of sacroiliitis and differential diagnosis.Methods Totally 509 patients with chief complaints of back pain and diagnosed as sacroiliitis from January 2007 to January in Chinese PLA General Hospital were enrolled.The clinical manifestations,laboratory examinations,imaging and pathological data of the 509 patients were retrospectively analyzed.Results There were totally 406 male patients and 103 female patients.Among all 509 patients,436 were diagnosed as ankylosing spondylitis (AS)/ spondyloarthropathy (SpA),including 385 men.Thirty-six cases were diagnosed as sclerosing osteitis.Ten cases were diagnosed as sacroiliac joint degeneration.Ten cases were diagnosed as pyogenic sacroiliitis or sacroiliac joint tuberculosis.Four cases were diagnosed as diffuse idiopathic bone hypertrophy.Four cases were diagnosed as palmoplantar pustulosis arthritis.Four cases were diagnosed as metabolic bone disease.One case was diagnosed as sacroiliac joint gout.Four cases were diagnosed as neoplastic disease.HLA-B27 positive rate was 86.9％ in AS/SpA.Erythrocyte sedimentation rate and C-reactive protein were increased mildly-to-moderately in AS/SpA patients.Erythrocyte sedimentation rate and C-reactive protein increased obviously in sacroiliac joint infection and tumor diseases.Imaging characteristics were sacroiliac joint bone destruction.Magnetic resonance imaging showed sacroiliac joint bone marrow edema,but range was not beyond anatomical structure in AS/SpA.Conclusions Based on disease distribution of sacroiliitis,AS/SpA is predominant while non-ankylosing spondylitis is not uncommon.Differential diagnoses of sacroiliitis should be considered to avoid malpractice,especially in patients with negative HLA-B27.
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Objective To study the changes of plasma cystatin C level (PcyC),and evaluate the effects of the joint use of atorvastatin and probucol on PcyC and severity of coronary lesion in patients with borderline lesion of coronary artery.Methods One hundred and thirty consecutive patients with borderline coronary lesion assessed by quantitative coronary angiography were enrolled into borderline coronary lesion group (BCL),and another 136 subjects without coronary lesion were enrolled as controls (CTR).And in the meantime,the subjects in BCL group were randomized (closed envelope method) into routine treatment subgroup ( RTT,n =60),and combined treatment subgroup in which patients were treated with atorvastatin 20 mg plus probucol 1.0 g daily in addition to routine medication ( CBT,n =70) for 6 months.There were no statistical differences in basic clinical features between two subgroups.PcyC,high-sensitive C-reactive protein (hs-CRP),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol ( HDL-C ) and triglycerides (TG) were determined.Of them,104 patients in BCL group rechecked by coronary angiography.Comparison of biomarkers carried out between two groups by using a number of independent-sample t-test and analysis of variance.For enumeration data,chi-square test was used to compare mean values of biomarkers between groups. P ＜ 0.05 was considered statistically significant.Results PcyC levels were significantly higher in BCL group than those in CTR group ( P ＜ 0.05 ).Compared with RTT subgroup,levels of PcyC,TC,LDL-C,TG and hs-CRP were more significantly decreased in CBT subgroup (P ＜ 0.05,P ＜ 0.01 ).Moreover,there was a trend of slight decrease in the mean percent of stenosis (MPS) of coronary artery with borderline lesion in RTT subgroup treated for 6 months,whereas more marked decrease in the MPS of coronary artery with borderline coronary lesion in CBT subgroup treated for 6 months ( P ＞ 0.05 ; P ＜ 0.05 ).Conclusions Cystatin C plays an important role in the pathogenesis of coronary artery,and PcyC is associated with severity of coronary lesion,the combination of atorvastatin and probucol decreases the PcyC level,and it may be the treatment of choice for borderline lesion of coronary artery.
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Objective:To establish an efficient method for expression and purification of vMIP-Ⅱ which encoded by a viral gene and ho-mogued human chemokine in active single strand in E.coli cells. Methods: Cloning with bi-enzyme restriction and lysising bacteria with cold osmoic shock were used for expression,MBP affinity chromatography of fusion protein and self-selective of recombinative peptide for final purification. The expressing and purifying products were detected with SDS-PAGE and Western blotting and identified with inhibitory adhesion experiment for its activity.Results: Fusion protein MBP-vMIP was effeciendy expressed in E. coli at secretive type, and self-cleaved to sparate the vMIP-Ⅱ. The final product single strand vMIP-Ⅱ was active for blocking chemokine receptor CCR5.Conclusion:This is an effective method for obtaining viral chemokine vMIP-Ⅱ of recombinant single strand.The recombinant vMIP-Ⅱ may be useful for the study of diseases involving in chemokiine receptors such as HIV infection,rejection of transplantation and chronic inflammation,etc.
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Objective To compare the thickness of the retina and its sublayers between young and elderly rats using a stereological method. Methods Six young(3 months old) and six elderly(2 year old) LongEvans rats were used in this study.The right eyeball was dissected from each rat and prepared as a set of serial sagittal sections and applied with HE staining.The sections and fields were sampled in the systematic random fashion and examined under a light microscope.The thickness of the whole retina and its 8 sublayers were identified and measured. Results Compared with that of the young rats,the thickness of the whole retina and most of the sublayers of the elderly rats were significantly decreased.The decrease was such so that the proportion of the thickness of each sublayer to that of the whole retina remained unchanged.Most interestingly,among the 8 sublayers of the retina,the thickness of the exterior plexus layer reduced nearly 46.2% during aging process.Conclusion Aging has a significant effect on the thickness of the rat retina.Such effect is better presented with the systematic random sampling method.