Effects of sIL-13Rα2 on the nasal mucosa goblet cell apoptosis of allergic rhinitis of rats / 中华耳鼻咽喉头颈外科杂志

Objective@#To investigate the effects of sIL-13Rα2 on the apoptosis of goblet cell in nasal mucosa of allergic rhinitis rats.@*Methods@#Forty healthy male Wistar rats were randomly divided into 4 groups (10 rats per group): control group (group A), AR group (group B), sIL-13Rα2 group (group C) and triamcinolone acetonide group (group D). Ovalbumin (OVA) and aluminum hydroxide were used to establish the AR rat model. After the establishment of AR rat models, 50 μl PBS, 100 μg/50 μl IL-13Rα2 and 3.5 μg/50 μl triamcinolone acetonide were respectively dropped into each nasal cavity of every rat two times a week from 4 to 10 week in group B, group C and group D. Group A was operated with saline instead of OVA. The nasal mucosa tissues were collected at 24 h after the final administration. AB-PAS staining method was used to detect the quantity and secretion of goblet cells in the nasal mucosa tissue of all groups. Immunohistochemistry method was used to detect the expression of Bax proteins.Apoptosis was detected by TUNEL method.ANOVA analysis was used to compare multiple groups, and LSD-t test was used to compare the two groups.Pearson correlation analysis was used to analyze the correlation between the Bax positive cell rate of goblet cells and the rate of apoptotic cells. The difference was statistically significant with P<0.05.@*Results@#Compared with group A, there were more goblet cells and hypersecretion of mucus in the nasal mucosa tissue of rats in group B while fewer in group C. The goblet cells in group C and group D were significantly fewer than that in group B (0.639 00±0.831 vs 0.956 7±0.980, 0.661 90±0.657 vs 0.956 7±0.980, t value was 2.748, 2.767, respectively, all P<0.05). The immunohistochemistry results showed that the positive expression rates of Bax protein in goblet cells of group C and group D were significantly higher than that in group B (0.880 2±0.125 vs 0.568 7±0.953, 0.938 4±0.200 vs 0.568 7±0.953, t value was -2.292, -2.685, respectively, all P<0.05). The apoptosis rates of goblet cell in nasal mucosa of group C and group D were also significantly higher than that in group B (0.516 0±0.079 vs 0.274 0±0.056, 0.535 4±0.829 vs 0.274 0±0.056, t value was -17.671, -2.225, respectively, all P<0.05). The expression of Bax protein and apoptosis of goblet cells were positively correlated (r=0.859, P<0.01).@*Conclusion@#sIL-13Rα2 can induce apoptosis of the goblet cells in nasal mucosa of allergic rhinitis rats, by inhibiting IL-13 and up regulating Bax.

Establishment of EA-D-IgA antibody detection method and its diagnostic efficacy for nasopharyngeal carcinoma in different clinical stage / 肿瘤研究与临床

Objective To establish a serological detection method for EA-D-IgA antibody,and to evaluate its diagnostic efficacy for nasopharyngeal carcinoma in different clinical stage.Methods EA-D-IgA antibody serological detection method was established by using the polypropylene microplate with eukaryotic expression product of BMRF1 whole gene fragment of EB virus.Fifteen early stage (stage Ⅰ and Ⅱ) and 48 advanced (stage Ⅲ and Ⅳ) patients with nasopharyngeal carcinoma in Shanxi Provincial Cancer Hospital and Shanxi Dayi Hospital from April 2012 to August 2017,and serum samples from 40 patients with rhinitis who were treated at Shanxi Dayi Hospital from October 2016 to October 2017 were examined respectively by using the constructed EA-D-IgA antibody detection method.The positive detection rate of EA-D-IgA antibodies in different groups was calculated.When the patients with rhinitis were used as the differential control,the diagnostic efficacy of this index for different stages of nasopharyngeal carcinoma was evaluated.Results EA-D-IgA antibody serological method was successfully established.The positive detection rate of EA-D-IgA antibody in early nasopharyngeal carcinoma,advanced nasopharyngeal carcinoma and rhinitis control was 60.0 ％ (10/15),68.3 ％ (33/48) and 5.0 ％ (2/40) respectively.The differences between early stage nasopharyngeal carcinoma and the rhinitis control,advanced nasopharyngeal carcinoma and the rhinitis control were statistically significant (x2 =20.625,P =0.000;x2 =37.017,P =0.000).The difference between early nasopharyngeal carcinoma and advanced nasopharyngeal carcinoma was not statistically significant (x2 =0.394,P =0.530).When compared with the patients with rhinitis,the diagnostic sensitivity,specificity,positive predictive value,negative predictive value was 60.0 ％ and 68.3 ％,95.0 ％ and 95.0 ％,81.8 ％ and 94.3 ％,86.4 ％ and 71.7 ％ respectively in early nasopharyngeal carcinoma and advanced nasopharyngeal carcinoma.Conclusion The method constructed in this study effectively improves the efficacy of EA-D-IgA antibody detection in serological diagnosis of nasopharyngeal carcinoma,which can be used as an adjunct for early diagnosis of nasopharyngeal carcinoma,yet not as a reference for clinical staging.

Correlation between serum homocysteine level and left ventricular geometry in essential hypertension / 中华全科医师杂志

Objective To investigate the correlation between serum homocysteine(Hcy)level and left ventricular geometric patterns in essential hypertensive patients.Methods Three hundred and sixteen patients with essential hypertension attending in Shanxi Dayi Hospital from January 2013 to June 2016 were enrolled in the study.Serum Hcy levels were measured and echocardiography was performed to measure left ventricular mass index (LVMI)and relative wall thickness(RWT).According to Hcy level patients were divided into H type hypertension group(Hcy≥10 μmol/L,group A)and non-H type hypertension group(Hcy<10 μmol/L, group B).The correlation of Hcy level with RWT and LVMI was analyzed.Results The percentages of concentric remodeling and concentric hypertrophy in group A were higher than those in group B(30.7% vs.25.3% and 24.7%vs.18.7%,χ2=3.342 and 3.894, P=0.033 and 0.025).Hcy level was positively correlated with RWT and LVMI(r=0.819 and 0.725,P=0.005 and 0.008).Conclusion Serum Hcy level is associated with left ventricular geometry in patients with essential hypertension.

Roles of penA and mtrR gene mutations in in vitro-induced resistance of Neisseria gonorrhoeae to ceftriaxone / 中华皮肤科杂志

Objective To investigate the roles of penA and mtrR gene mutations in resistance of Neisseria gonorrhoeae to ceftriaxone.Methods Standard strains of Neisseria gonorrhoeae (ATCC-49226),clinical strains of Neisseria gonorrhoeae with high sensitivity to ceftriaxone (2012-4052 and 2012-15361) and clinical strains of Neisseria gonorrhoeae with reduced sensitivity to ceftriaxone (2012-5616) were treated with ceftriaxone at subinhibitory concentration (50％ MIC),so as to induce the resistance to ceftriaxone.DNA was extracted from the primary strains before the treatment and daughter strains resistant to ceftriaxone after the treatment,followed by the amplification and DNA sequencing of the penA and mtrR genes.Results For strains 2012-5616 and ATCC-49226,ceftriaxone-resistant strains with MIC ≥ 1 mg/L were obtained after 26 and 28 passages,respectively.For strains 2012-4052 and 2012-15361,ceftriaxone-resistant strains with MIC ≥ 0.5 mg/L were obtained after 22 and 36 passages,respectively.Sequence analysis of the penA gene revealed that A501T and G542S mutations were identified in the induced resistant ATCC-49226 strains,but no new mutations were observed in the other 3 strains.All the 4 mutant strains showed penicillin-binding protein 2 (PBP2) of gene sequence type ⅩⅧ and no mosaic structure of the penA gene was found in the strains.Sequence analysis of the mtrR gene showed that the A39T mutation was found in the 2012-5616 and ATCC-49226 strains before and after the induction,as well as in the coding region of the mtrR gene in the induced resistant 2012-4052 strains.Conclusion The A501T and G542S mutations in the penA gene and A39T mutation in the mtrR gene may play a role in the resistance of Neisseria gonorrhoeae to ceftriaxone.

Integrated application of hospital information based on enterprise service bus / 中华医院管理杂志

The present hospital information systems are analyzed,with finding that hospital information isolated island phenomenon getting worse due to failure of interconnection of various systems and equipment vendors with different technologies.An integrated platform based on an enterprise service bus is proposed for hospital information,which can be connected into these heterogeneous systems,effectively minimizing the inter-system coupling,and achieving data and service interconnection,enabling an integrated and intelligent hospital architecture.

An analysis of the sleep condition and its influence factors in old patients with hypertension / 中国实用护理杂志

Objective To investigate the sleep condition and its influence factors in old patients with hypertension. Methods A total of 100 old patients with hypertension were investigated by Pitts-burgh sleep quality index (PSQI) and serf-designed questionnaire of influence factors. Results 76 per-cents of the old patients with hypertension had sleep disorders.The total score of PSQI,sleep quality,time take to fall asleep,hours of sleep,sleep effficiency,sleep disorders,hypnotic drugs and every score of daytime dysfunction were significantly higher than domestic norm(P<0.01).Surroundings(strange circumstances or noisiness), physio-pathology (disease distress or high frequency of going to toilet during nighttime),psycho-logical situations(the fear to the disease or worrying about medical fees) and drags(use of diuretics or the adverse effects of some antihypertensive agents) were the important factors affecting sleep quality. Con-clusions Old patients with hypertension have poor sleep quality,which relates with multiple factors.Valid nursing interventions should be adopted in order to control the various factors which affect sleep quality to improve patients' sleep quality.

Assay and clinical significance of serum tumor markers in diagnosis of pancreatic carcinoma / 肿瘤研究与临床

Objective To investigate the expression of serum tumor markers in pancreatic carcinoma and to assess the value of serum tumor markers in the diagnosis and prognosis of pancreatic carcinoma. Methods Five serum tumor markers including AFP, CEA, CA199, CA125 and CA242 were detected in 95 patients with pancreatic carcinoma by chemi-luminescence immunoassay (CLI) and enzyme linked immunosorbent assay (ELISA), 80 patients with other malignant tumor and 65 patients with benign disease served as controls. Five tumor markers in pancreatic carcinoma diagnosis for TNM staging were compared. The value of tumor markers for prognosis was analyzed. Results Among these 5 kinds of tumor marker, CA199, CAI25, CA242 were valuable in diagnosis of pancreatic carcinoma, and CA199 was the most valuable one, whose sensitivity and specificity were 76.2 % and 74.8 % respectively. CA199, CA125, CA242 were increased with progression of pancreatic carcinoma. Conclusion Serum CA199, CA125 and CA242 are the preferred tumor markers to be used in the diagnosis and prognosis of pancreatic carcinoma.

Utility of yag laser in naso-antrostomy

Adequate drainage with good ventilation is the key problem in the management of chronic maxillary sinusitis. However, conventional operation of Caldwell-Luc has been limited by the failure to maintain unimpeded and permanent antrostomy. Laser surgery has certain advantages over the scalpel and cryoprobe in treating chronic purulent sinusitis, especially in those patients where an operation of Caldwell-Luc is indicated. Since 1987, 62 sides of naso-antrostomy in 49 patients with chronic maxillary sinusitis are performed by means of YAG laser technique. Laser surgery is done in the form of either middle or inferior meatus antrostomy when appropriate. Intra-operative bleeding is minimal. No stenosis of antrostomy is found after following for 10 to 36 months. The authors believe that this method of laser nasoantrostomy is safe, convenient, bloodless, and needs no postoperative nasal packing. The utility of YAG laser coagulation and vaporization is capable of creating a sufficient permanent antrostomy in the management of chronic purulent maxillary sinusitis

Quality evaluation of Shandong Radix Scutellariae processed / 中成药

AIM: To inspect the quality of field processed products of Radix Scutellariae in Shandong Province. METHODS: TLC and UV identification, ethanol-soluble extract, moisture content, total ash and acid-insoluble ash and baicalin were determined. RESULTS: The field processing is superior to traditional processing methods. CONCLUSION: Field processing of Radix Scutellariae in Shandong province has feasible standardization and industrial benefits.