Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.</p><p><b>METHODS</b>A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.</p><p><b>RESULTS</b>To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.</p><p><b>CONCLUSIONS</b>The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.</p>

Investigation and analysis of occult hepatitis B virus infection among voluntary blood donors in Lishui area / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Occult hepatitis B infection of voluntary blood donors has been plagued in the serum screening. Determined the OBI through the highly sensitive detection methods Nest-PCR among the blood donors, and then learned occult HBV infection and analysed the genotypes of this area.</p><p><b>METHODS</b>10 080 serums of donors were determined respectively by the imported Abbott HBsAg kit and Beijing Wantai anti-HBc and anti-HBs reagents, obtained the gene and detected DNA sequences by the high sensitive Nest-PCR method.</p><p><b>RESULTS</b>Among 10 080 cases of unpaid blood donors, 108 cases were detected HBsAg positively by Abbott sensitivity kit (positive rate of 1.07%), 767 cases were anti-HBc single - positive (positive rate of 7.67%). 25 patients screened blood donors who tested negative for serum HBsAg and positive for HBV DNA in the 10 080 cases. Occult HBV infection incidence rate was 0.25%. 12 cases were HBV genotype C (48%), 13 cases were genotype B (52%), and no other genotypes. Genotype B has no statistically significant difference to genotype C (P > 0.05). Sequence analysis showed that 5 patients in the HBsAg epitope "a" (aa124 - aa147) have mutation (20%).</p><p><b>CONCLUSION</b>The high proportion of occult hepatitis B infected among voluntary blood donors in our country. Also genotype and mutation was differences in different regions.</p>