RESUMO
After biocontrol Trichoderma longbrachiatum T2 was applied in the rhizosphere of cucumber, the functional diversity of the rhizomicrobes utilizing carbon sources was investigated by using Biolog Eco-microplates technique. The result showed that T. longbrachiatum T2 had a significant effect on the rhizomicrobes in terms of their metabolic activity and their capacity to utilize carbon sources. The ability of the rhizomicrobes to utilize carbon sources was enhanced at seedling stage. The significant change of the richness and the degrees of the rhizomicrobes occured at seedling stage, flowering stage, and final stage, while no obvious difference was observed at fruiting stage. Principal Component Analysis (PCA) indicated that the ability of the rhizomicrobes to utilize carbon sources was enhanced at seedling stage and final stage. At flowering stage, the ability to utilize carbohydrates, amino acids, carboxylic acids, amines and phenolic compounds was reduced, but the ability to untilize polymers was slightly enhanced. The ability to utilize carbohydrates, amino acids, carboxylic acids, polymers and amines was enhanced, but the ability to utilize phenolic compounds was reduced at fruiting stage.