Histopathological and immunohistochemical study of giant cell granuloma of the jaw and giant cell tumor of long bones [comparative study]

Central giant cell granuloma [CGCG] and peripheral giant cell granuloma [PGCG] are tumor like lesions that affect jaw bones, while giant cell tumor [osteoclastoma] is a tumor that affects the long bones [e.g. tubular bones]. Its affection of the jaw bones is a matter of debate. Both are very similar in their histopathological features while they vary in their clinical behavior. GCT shows a more aggressive behavior than GCG. To evaluate the expression of [Proliferating cell nuclear antigen] and [P53] in peripheral and central giant cell lesion of the jaw and giant cell tumor of long bones with correlation to histopathological parameters. A total of 17 [GCT], 15 [CGCG] and 16 [PGCG] cases where enrolled in this study. Immunohistochemical staining with PCNA and P53 monoclonal antibody was performed. A non-significant difference m proliferative activities was recorded among different histological giant cell lesion subtypes. Giant cell granuloma expressed the same proliferative potential to that of giant cell tumor, moreover PCNA expression was not statistically correlated to different histopathological paramters of lesion subtype. On the other hand. The anti-apoptotic potential of giant cell granuloma which expressed by anti P53 monoclonal antibody was the same of that of giant cell tumor. Results of this study proved that the biological behavior namely P53 and PCNA activities was comparable between giant cell lesions and giant cell tumor. This suggest that these two conditions may act as one disease entity with a spectrum of clinical behavior, possibly due to certain differences in anatomical location which by itself affect its biological behavior. This hypothesis needs further verification concerning the clonality of the lesion to be accepted or refused

Immunohistochemical expression of matrix metalloproteinase-9 in jaws and long bones osteosarcomas

Matrixmetalloproteinases [MMPs] are a class of matrix basement membrane degrading enzymes which were shown to be associated with metastases in several human tumors. This study is aimed to investigate the potential effect of MMP-9 in imparting the frequently reported different behavioral pattern between jaws and long bones osteosarcomas. Tissue blocks of ten cases of jaws osteosarcomas and another ten of long bones osteosarcomas were collected and stained immunohistochemically with monoclonal antibodies to MMP-9. The majority of cases [70%] were positive for MMP-9 expression which indicates a role in tumor spread, however; there was no significant difference between the sites. The study indicates that MMP-9 is probably not involved in the biologic differences between jaws and long bones osteosarcomas

Nucleolar organizer reglons in the assessment of meoplastic and non-neoplastic thyroid nodules

Forty seven cases of thyroidectomy specimens were selected for this study including 6 cases of anaplastic carcinoma, 9 cases of papillary carcinoma, 6 cases of follicular carcinoma, 8 cases of follicular adenoma and 18 cases of adenomatous goiter. Paraffin section from each case are stained by the one step silver colloid reaction to demonstrate the argyrophilic nucleolar organizer region associated proteins. Anaplastic thyroid carcinoma had mean AgNOR count per cell distinctly and significantly higher than other categories. Mean AgNOR counts of papillary carcinoma, follicular adenoma, follicular carcinoma and adenomatous goiter showed considerable overlap. It is concluded that AgNOR counting has limited value in the differentiation between neoplastic and non-neoplastic thyroid nodules