PC3 prostasome promotive effects on fresh or cryopreserved human spermatozoa in swim-up medium / 中华泌尿外科杂志

Objective To investigate the motility stimulant effects of prostasome like granules from the PC3 prostate cancer cell line (here called PC3 prostasome). Methods Fresh or cryopreserved human spermatozoa in Earle's balanced salt solution (EBSS) supplemented with human serum albumin (HSA,10 mg/ml) or PC3 prostasome (0.10 mg/ml or 0.25 mg/ml)after swim up to compare the difference of motility or the recovery of motility. Results For the fresh samples within different time of incubation or different parameters in one hour incubation,addition of PC3 prostasomes 0.10 mg/ml was superior to HSA in sperm motility stimulation ( P